EMAGE mouse gene expression spatial database

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EMAGE:11508

Nub1 negative regulator of ubiquitin-like proteins 1 ( MGI:1889001)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006658. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006658_01	euxassay_006658_02	euxassay_006658_03	euxassay_006658_04

euxassay_006658_05	euxassay_006658_06	euxassay_006658_07	euxassay_006658_08	euxassay_006658_09

euxassay_006658_10	euxassay_006658_11	euxassay_006658_12	euxassay_006658_13	euxassay_006658_14

euxassay_006658_15	euxassay_006658_16	euxassay_006658_17	euxassay_006658_18	euxassay_006658_19

euxassay_006658_20	euxassay_006658_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11508_wholemount_strong.wlz

11508_wholemount_moderate.wlz

11508_wholemount_weak.wlz

11508_wholemount_possible.wlz

11508_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11508_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

cerebral cortex marginal layer

strong

regional

strong expression: see section 07 08 09 10 11 18 19 moderate expression: see section 01 02 03 04 05 06 12 13 16 17 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7122
Entity Detected:	Nub1, negative regulator of ubiquitin-like proteins 1 ( MGI:1889001)
Sequence:	sense strand is shown >T7122 CCACGCGTCCGGCAGTTTTACTTTCAAGGATGGCGCAGAAGAAATATCTTCAAGCAAAACTGACTCAGTT TCTAAGGGAAGACAGAATTCAACTTTGGAAACCTCCATATACTAAAGAAAATAAGGAAGTTGGTTTGGCC GTAAAGGACCTTGCTAAGAAGTACTCTGAGCGGCTGGAGTGCTGTGAAAACGAAGTGGAAAACATAATCG AGGAAATCCGACGCAAAGCAATTGAGCGCGGCACTGGGAATGAACACTACCGAACAACTGGGATCGCCAC CATTGAGGTGTTCCTGCCTCCAAGGCTCCGGAAGCACGATAAGAAAAGCTTGTTGGAGACCCGCTTGCAT GTCACTGGTAGAGACCTGAGGTGTCAAATAGCTGAAACTTTCGGATTTCAAGAAAATTACATCAAGATCG TAATAAATAAGAAACAGCTTCAACTAGGGAAAAGCCTTGAAGAACAAGGAGTCACTCACAATGTGAAGGC GATGGTACTGGAGCTGAAACAGTCTGAAGAGGATGTGAGGAAAAACTTACAGCTAGAGGAAGAGGAACAG AACGAGGCCGAGCTGAAGGAGAGACGCATTCAGAGGACCAAACGGGGACTGGAGATCCTGGCGGAGAGAG CTGAGATGGTGGTTGATCCGGAAACGATGCCTTACTTAGACATTGCGAACCAGACAGGCAGGTCACTCAG AATTCCCCCTGCAGAAAGAAAAGCCCTTATGTTAGCTATGGGATATCACGAGAAGGGCAGAGCTTTCCTG AAAAGAAAAGAGTATGGAATAGCCCTGCCCTGCCTCCTGGACGCTGACAGATACTTCTGTGAGTGCAAAG AGCTGCTGGACACTGTGGACAACTATGCAGTCCTCCAGCTGGACATTGTGTGGTGTTACTTCCGCCTGGA ACAGCTGGAATGCCTTGATGATGCAGAGAAAAAGCTGAACTTGGCCCAGAAATGCTTTAAAAACTGTTAC GGAGAGAATCACCAGAGACTGGTCCACATAAAAGGAAATTGTGGGAAAGAGAAGGTGTTGTTTTTAAGAC TCTACTTGCTTCAGGGAATCCAAAACTATCACAGTGGAAATGGAGAGGAAGCTCGGGAATATCTCAACAA GGCACGGCAGCTCTTTAAGGAGCTTTACATCGATCCATCAAAAGTTCACAACTTGTTACAGTTGGGATTC ACTGCCCAGGAAGCACGGCTGGGCCTACGGGCCTGCGATGGAAACGTGGACCATGCGGCCACTCACATTT CCAACCGCAGAGAGGAACTGGCCCAAATCCGGAAGGAAGAGAAGGAGAAAAGGAGACGCCGTCTGGAAAA TGTCAACACTTTGAGAGGAATGGGCTACTCCACACAAGCTGCCAAACAAGCCCTTCACCAGGCCAGAGGC AACCTGGACGATGCCCTGAAGGTTCTCCTCAGCAACCCCCACATGTGGTGGTTACAGGATGCAGACCCTG AAAACAATAGCCGCCAAGCAAGTCCTTCCCAGGAAAGCATCAACCAGCTGGTGTACATGGGCTTTGACAC AGTGGTGGCTGAAGCTGCACTAAGGGTGTTTGGAGGCAATGTCCAGCTGGCAGCTCAGACCCTTGCACAC CATGGAGGAAGCCTCCCACCCGACCTGCAGTTCTCAGGAGAGGACTCCTCCCCCACACCATCCACATCCC CATCTGACTCTGCAGGGACCTCTAGTGCCTCGACAGATGAAGACATGGAGACGGAGGCTGTCAACGAAAT CCTGGAGGACATTCCGGAGCACGAGGAGGACTACCTGGACTCCACGCTGGAGGATGAAGAAGTCATTATT GCTGAATACTTGTCCTACGTTGAAAGTATAAGTTCTGCCGCAAAGAACAACTGATCAGAAATAAGTGCTA CGTTTCGGCTATAAATTCTGTGGTTGTAAATGGTTGAATGCTTCTTTGCGTTCCTCTTGCTTTTCAACGA TTTTTCTCTAAGTAGGCTTCCTCCTCAGGTACAGGGACCCAGTGTATTGAAGAGACTTTGTCAGTCAGAG GACAGTCTAGGAAAGGGAATGTCCCAGCCTAGCTCTGCTGTGCCCTGTCCTCCTCTAGGCCTGCATCCCA GACTGCCACTCTGTTGGTTTTCCATTCAGTGTAACAGGGAAGCAGGTCCCCCCACTTCCTGAGGCAGGGT AGGTAGTCCCGGAGAAGGTCTGCAGAGGGCCTGGGACTGTCTCCTGTCGTCTTCTCAGCTAGCCATCCTG TGCACTTTGCCCACTGTCCTGCTGCTGAACTGCCCTCCTCCCCCACCACCTCTGCTGTGCAAGCTTCTCT CACCCTTGCTGCCCTGCATCACCCATTGCATCTTCATTCCAAGTGCTGTGGAGCCCAGCAATGACGGACA GGCTGCCAGCCTCCCTCCCACCGTGGCAGGCGGTGCAGATCCCTTGATCGAAAAATGACAGTGTTTTATG CTCTCAGATCAGTAAAATCTGTACTTGAAATTAAGGTTGCAAAATCAGTTTATATGGATTCTAAATTTCT CATACAAAGTGAAAATCTTATTAAATGGAAAAAATTTAAATGCTAACCACAGCAAGTTATTTTTAATTAA CATCTCTAGACTGGAAGCTTACTGATAGCCAAGGGACCTGAGATTGCGATCTGCTTTCTCAGGACTCTCC CTGGGTTTCCTATAACTGGGTCACAGAAGACTTGCTGAAGAAGGGCCTGTCAGCCTTTGGTTGCTTAGTC CTGGGTGTGGCCAGGCAGATAGCCTTCTAGCACCCAGGCATGGTAACAGCCAGATGTGGTGTGGGCCAAG TGCCACTGTGTTCCCAAGAGCTTTTCATTTCAGCTGAAGCCTTGGCAGCAGACCAAGCTGGACAAGTATG ATTGTCTCTAGCTATTTTTTTTTTTTTTAATTACACATTTGTGTGGGGGATAGGGCAGACATACCACAGC ACGAGTCTGGGGCTCAGAAGACAATGTGTGGACTGAACTCAAGCTGGCCCCGCCTCCTGATGGTCTTGCT GGCCCCCTTGCTGTGCTTTGGAACTGAATGATGCTGATGGAAATAAACATGGGGAACGCACTTGAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4018648 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4018648 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11510 same embryo
	EMAGE:11507 same embryo
	EMAGE:11509 same embryo
	EMAGE:11511 same embryo
	EMAGE:11506 same embryo
	EurExpress:euxassay_006658 same experiment
	MGI:4826825 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source