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EMAGE:11600

0610011F06Rik RIKEN cDNA 0610011F06 gene ( MGI:1915597)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:11600 EMAGE:11600 EMAGE:11600 EMAGE:11600 EMAGE:11600
"Pseudo-wholemount" of euxassay_006781. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_006781_01 euxassay_006781_02 euxassay_006781_03 euxassay_006781_04
EMAGE:11600 EMAGE:11600 EMAGE:11600 EMAGE:11600 EMAGE:11600
euxassay_006781_05 euxassay_006781_06 euxassay_006781_07 euxassay_006781_08 euxassay_006781_09
EMAGE:11600 EMAGE:11600 EMAGE:11600 EMAGE:11600 EMAGE:11600
euxassay_006781_10 euxassay_006781_11 euxassay_006781_12 euxassay_006781_13 euxassay_006781_14
EMAGE:11600 EMAGE:11600 EMAGE:11600 EMAGE:11600 EMAGE:11600
euxassay_006781_15 euxassay_006781_16 euxassay_006781_17 euxassay_006781_18 euxassay_006781_19
EMAGE:11600 EMAGE:11600 EMAGE:11600 EMAGE:11600
euxassay_006781_20 euxassay_006781_21 euxassay_006781_22 euxassay_006781_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:11600Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
11600_wholemount_strong.wlz
11600_wholemount_moderate.wlz
11600_wholemount_weak.wlz
11600_wholemount_possible.wlz
11600_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:11600_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
embryo
strong strong
ubiquitousstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T8228
Entity Detected:0610011F06Rik, RIKEN cDNA 0610011F06 gene ( MGI:1915597)
Sequence:sense strand is shown

>T8228
CCGCACCGCTGCGATGATGATGGCTGCGGCTGCTGAGAGAAACAAGGAGCCCATCCTGTCCGTACTCCGC
CAGTATGTGGATCCTGCCCAGCGCTGTGTTCGCGTGCTCGAGGTGGCCTCGGGCTCTGGACAGCACGCGG
CACACTTTGCACAGGCCTTCCCGAATGCCGAGTGGCAGCCGTCGGACGTGGACCAACGCTGCCTGGACAG
CATTGCCGCCACCACCCGAGCCCAAGGGTTGTCCAATGTGAAGGCTCCGCTGTACCTGGACGTGACCTGG
GAGTGGGAGCAATGGGGCGGCATCCCACCCCGATCGCTGGACCTGTTGCTCTGCATCAATATGATCCACA
TCAGCCCCCTGAACTGCACAGAGGGGCTCTTTAGAGCAGCAGGACACCTGCTCAAAACCAAGGCTGTGCT
GATCACCTACGGGCCCTATGCCGTCAATGGGAAGATCTCTCCCCAGAGCAACGTGGACTTTGACCTGACC
CTCAGATGCAGGAACCCAGAGTGGGGGCTTCGTGACACAGTCCTTCTGGAGGAACTAGGCCAGGCCAGTG
GCTTGGTCCTGGAGAGGATGGTGGACATGCCAGCCAACAACAAGTGCCTGATTTTTCGGAAAGAATAGCA
TCTCTGTCAAAAGTTGTCACGGTACAAGCTAGACCTCAGCCCCTCCCTCAGTCCTTGGGATAAATTAGCC
CCTCCTGGTCTGGTGGCCACAGGGCTGTAGAGAACCTGTTCTGAAGGGGCCTTAAAATAAATCCTTTCCA
GCCCACTGTGGGCTATCTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAA
Notes:The probe template was PCR amplified from IMAGE:1248589 using vector (pT7T3D-PacI) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:1248589 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:11601 same embryo
 EMAGE:11597 same embryo
 EMAGE:11596 same embryo
 EMAGE:11598 same embryo
 EMAGE:11599 same embryo
 EurExpress:euxassay_006781 same experiment
 MGI:4822582 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS