EMAGE mouse gene expression spatial database

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EMAGE:11614

A330049M08Rik RIKEN cDNA A330049M08 gene ( MGI:2444888)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_003653. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_003653_01	euxassay_003653_02	euxassay_003653_03	euxassay_003653_04

euxassay_003653_05	euxassay_003653_06	euxassay_003653_07	euxassay_003653_08	euxassay_003653_09

euxassay_003653_10	euxassay_003653_11	euxassay_003653_12	euxassay_003653_13	euxassay_003653_14

euxassay_003653_15	euxassay_003653_16	euxassay_003653_17	euxassay_003653_18	euxassay_003653_19

euxassay_003653_20	euxassay_003653_21	euxassay_003653_22	euxassay_003653_23	euxassay_003653_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11614_wholemount_strong.wlz

11614_wholemount_moderate.wlz

11614_wholemount_weak.wlz

11614_wholemount_possible.wlz

11614_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11614_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

strong

regional

strong expression: see section 01 02 03 04 05 06 07 21 22 23 24

humerus

moderate

regional

moderate expression: see section 03 04 05 20 21 22 23

hindlimb digit 1 metatarsal

strong

regional

strong expression: see section 03 23

hindlimb digit 1 phalanx

strong

regional

strong expression: see section 03 22

hindlimb digit 2 metatarsal

strong

regional

strong expression: see section 02 03 23

hindlimb digit 2 phalanx

strong

regional

strong expression: see section 03 21 22 23

hindlimb digit 3 metatarsal

strong

regional

strong expression: see section 02 03 23

hindlimb digit 3 phalanx

strong

regional

strong expression: see section 03 21 22 23

hindlimb digit 4 metatarsal

strong

regional

strong expression: see section 03 23

hindlimb digit 4 phalanx

strong

regional

strong expression: see section 03 21 22 23

hindlimb digit 5 metatarsal

strong

regional

strong expression: see section 04

hindlimb digit 5 phalanx

strong

regional

strong expression: see section 22 23

hip mesenchyme

strong

regional

strong expression: see section 01 02 10 18 19 24

knee mesenchyme

strong

regional

strong expression: see section 01 02 10 18 19 24

lower leg mesenchyme

strong

regional

strong expression: see section 01 02 10 18 19 24

upper leg mesenchyme

strong

regional

strong expression: see section 01 02 10 18 19 24

femur

strong

regional

strong expression: see section 07 08 09 11 20 21 weak expression: see section 04

pectoral girdle and thoracic body wall musculature

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 16 17 18 19 20 21 22 23 24

otic capsule

strong

regional

strong expression: see section 01 02 03 04 05 06 18 19 20 21 22 23

hyoid

strong

regional

strong expression: see section 09 10 weak expression: see section 11

stomach

strong

regional

strong expression: see section 08 10 moderate expression: see section 05 06 07

hindgut

weak

regional

weak expression: see section 14 15 16

rectum

weak

regional

weak expression: see section 14

midgut

weak

regional

weak expression: see section 07 08 09 10 11 12 13 14 15 16 17

lower jaw incisor

moderate

regional

moderate expression: see section 11 13 14 15

lower jaw molar

moderate

regional

moderate expression: see section 10 11 12 15 16 weak expression: see section 08 09

bladder

moderate

regional

moderate expression: see section 13 14 15

urethra of male

weak

regional

weak expression: see section 13 14

trachea

strong

regional

strong expression: see section 11 12 13

axial skeleton

strong

regional

strong expression: see section 08 10 11 12 13 14 15 16 moderate expression: see section 07 09 17

basisphenoid bone

strong

regional

strong expression: see section 06 07 08 09 15 16 17 23 24

exoccipital bone

strong

regional

strong expression: see section 01 22 23 24

temporal bone

strong

regional

strong expression: see section 23 24

orbito-sphenoid

strong

regional

strong expression: see section 01 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

viscerocranium

strong

regional

Expression in the turbinate bone.

scapula

moderate

regional

moderate expression: see section 03 04 21 22

axial skeleton tail region

strong

regional

strong expression: see section 12 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6603
Entity Detected:	A330049M08Rik, RIKEN cDNA A330049M08 gene ( MGI:2444888)
Sequence:	sense strand is shown >T6603 GGGGTGTGGAGCAGGTAGGGCGCAGGGCACGGAACGCGGGGGCTTGGAAGGACAGCGCACAGGGGCTCAG GCCCGCGGAAGATAGCTCTCCGGACCCCCAGGGGTCCCCAGCCTGGCGCGCGCCTTGGCGTGAGCCGGTA GCTGAAAGGTCAGCAACATCTCCCGGTTGTCTGCAGACACCGGTGCACAGAGGTGTTCAGGGTGCAAGCT GAGAAGCACCCCGAAGCTGGGTGCATTCGGGGCTACATCTCTGGCATGCGGAAAGCTGGTGGTTTCCTTG TCCGCTCCTACGAAGTCACCGGGAGCGACCTGGACTCCCGGAAGAAAAGCCTACGAGAGGAACAGAGATG CCATTGAGAGCTGTCGCTGCCGGCATGAGGCAAGATCGGCTCTGGGGTTGCTCACTGTTCTTCTTGAAGG AGGAGTGACTGGCCGCCACCGGCACCTGGAACCCAGCACCCAGGAGGTGAACTCGGACGGACCTGAGGAG GATCCTGTGTCCTGTGTCCTTGGGAGACTACTCACTGGTGCATCTCAGGGCAGAGGAGTGGCAGAACACG GGCATTCCACGGGGCTGCTCTTCAGCTTGCAGACTAACTGAGCACAAGAGTGCGCTTTGTAGCTCTGAGC CTCAGGTCCCGATGGGAAGCCATGGGAAAGGAAACCCTGTTTTCTGTGGCATCTTGTACTGGGAGCAGGG GCGAGATGACCACAGCCACCACTTTGGGGGACGCCGTCTTCTCGCTGAACATGACCAGGGGAGAGGACGC CCTGTATAAGAGCTCTGGAGCCATCGTGGCTGCCATCGTGGTGGTTGTCATCATCATTGTCACCTTGGTG CTGATCCTGCTGAAGATGTACAACAGGAGAATGAGGACCAGGCGGGAGCTGGAGCCCAAGAGCCCAAAGC CACCTGTCCCTCCTGCCCTGGACCCCAGCAGCAATGGCAGCCAGCAGCCTGCTACTGTGACCTTCGACCC TGCCAATGTCCATGTGGAGACTCGGTGACCCTGCCTTGGCGCCATTCCTGCCTCTCTGCAAAGAGCTTTG ATGACCTGAAAGTCCACTCTGCTGGCAGCTTCACCAAGTTCTTTCTGGTTGGGTAAACTGAGGCACCCTT CAAACAGCCCCTGCAGCTTGAAGCCACCCTCACCTGTGCTCTGGCCACCCCAGCTATGTCCACGTCCCTG CTGCCACTCGACCAAAGCTCTGAGGACATTTCTTCTGTTACCTGACGAGAAGCACTGAGCTGGGGAAACT GGTGTGAGTCTGGTTGTCGCCTCACAAGTGCGACGGCTAGACAGCTTGATAGACAGACATCTGAGTCTTC TGCTCATGGTCAGGGAGTGTTAAAGAGAGTGTTCCCCAGGGAGCCACTGGGAGCATCTCAACTTAAGGGC ATGTCCACAGCTTCCTCAATCCCATAGCCAGATGCAGGGAAACTAACTTGGACTAACTAGCCACAAAACC AACTTGGTAGCATCTAACTGATGCAGTACTAGATGGCCACTAGCCAGCTCCTAATGGCCAGTCAGCGCTC TCCCCCTTTAACAAAGGGTACAGAGGCTGGATAAAGTGGCACGTGCCTGTCATCACAGTGCTCAGTAAAA GCGGGAACACTGGGATTGCAGGACCAGCTTTGACCACATTCAGAGATGCTTCTCTTTAAAAAAAAAAAAA AA
Notes:	The probe template was PCR amplified from IMAGE:3595770 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3595770 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11617 same embryo
	EMAGE:11616 same embryo
	EMAGE:11613 same embryo
	EMAGE:11615 same embryo
	EMAGE:11612 same embryo
	EurExpress:euxassay_003653 same experiment
	MGI:4822829 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source