EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:11618

Pitrm1 pitrilysin metallepetidase 1 ( MGI:1916867)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006516. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006516_01	euxassay_006516_02	euxassay_006516_03	euxassay_006516_04

euxassay_006516_05	euxassay_006516_06	euxassay_006516_07	euxassay_006516_08	euxassay_006516_09

euxassay_006516_10	euxassay_006516_11	euxassay_006516_12	euxassay_006516_13	euxassay_006516_14

euxassay_006516_15	euxassay_006516_16	euxassay_006516_17	euxassay_006516_18	euxassay_006516_19

euxassay_006516_20	euxassay_006516_21	euxassay_006516_22	euxassay_006516_23	euxassay_006516_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11618_wholemount_strong.wlz

11618_wholemount_moderate.wlz

11618_wholemount_weak.wlz

11618_wholemount_possible.wlz

11618_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11618_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

axial musculature

strong

regional

strong expression: see section 03 04 05 06 07 19 20 21 22 moderate expression: see section 23

adrenal gland

strong

regional

strong expression: see section 09 10 17 moderate expression: see section 08 18 19

thymus primordium

strong

regional

strong expression: see section 10 11 12 13 14 15

submandibular gland primordium

strong

regional

strong expression: see section 05 06 07 08 09 16 17 18 19

vibrissa

strong

regional

strong expression: see section 04 05 06 07 08 18 19 20 21 22

telencephalon ventricular layer

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

midbrain ventricular layer

strong

regional

strong expression: see section 07 08 09 10 11 12 13 14 15 16 17

lower jaw incisor

strong

regional

strong expression: see section 10 11 14 15 16

lower jaw molar

strong

regional

strong expression: see section 06 18 moderate expression: see section 19

upper jaw incisor

strong

regional

strong expression: see section 10 11 14 15 16

upper jaw molar

strong

regional

strong expression: see section 06 18 moderate expression: see section 19

liver

strong

regional

strong expression: see section 05 06 07 09 10 13 14 15 17 18 19 20 21 moderate expression: see section 01 02 03 04 08 11 12 16 22 23 24

metanephros

strong

regional

strong expression: see section 09 10 17 20 21 moderate expression: see section 07 08 11 18 19 22

lung

strong

regional

strong expression: see section 02 03 04 05 09 10 13 17 20 21 moderate expression: see section 06 07 08 11 12 14 15 16 18 19 22 23 24

orbito-sphenoid

strong

regional

strong expression: see section 01 02 03 04 05 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6667
Entity Detected:	Pitrm1, pitrilysin metallepetidase 1 ( MGI:1916867)
Sequence:	sense strand is shown >T6667 GGGATCGCAGAGAAAGATGTCAAGACGGTCAGAGAGCTCGTAGACAGGACAATCGAAGAAGTTATAGAGA AAGGATTTGAAGATGATCGGATTGAAGCTCTGCTTCATAAAATCGAAATTCAAACGAAGCATCAGTCAGC CAGCTTTGGCCTGACCCTGACGTCATATATAGCTTCTTGCTGGAACCATGATGGGGACCCTGTGGAGCTC CTGCAGATTGGAAGTCAGCTGACTAGATTTAGGAAGTGCCTTAAGGAAAATCCAAAATTTTTACAAGAAA AAGTAGAACAATATTTTAAGAACAATCAGCACAAGCTGACTTTATCCATGAAGCCAGACGACAAGTATTA TGAAAAGCAAACTCAGATGGAGACAGAAAAGCTGGAGCAAAAGGTGAATTCTCTCTCCCCGGCGGACAAG CAGCAGATCTACGAGAAAGGTTTAGAACTACAGACGCAGCAAAGTAAACATCAAGACGCCTCCTGCCTCC CAGCATTGAAAGTCTCGGACATTGAGCCCTCCATGCCTTTCACCAAGCTTGACATCGGCCTTGCAGCTGG AGACATCCCTGTGCAGTACTGCCCACAGCCCACCAACGGCATGGTGTATTTCCGAGCCTTTTCCAGTTTA AACACGCTGCCGGAGGACCTGAGGCCCATTGTGCCTCTCTTTTGCAGCGTGCTGACCAAGCTGGGTTGTG GCATCCTTAACTACAGAGAGCAAGCCCAACAGATTGAGCTCAAGACAGGAGGCATGAGTGTCACGCCCCA TGTGCTCCCTGACGACTCACAGCTGGATACCTACGAGCAGGGTGTGTTATTTTCATCTCTCTGCCTGGAG CGGAACCTGCCAGACATGATGCATCTGTGGAGCGAAATATTTAACAATCCATGCTTTGAAGAAGAAGAAC ACTTCAAAGTGTTGGTGAAGATGACCGCTCAGGAGCTCTCCAATGGAATTTCAGACTCGGGGCATCTCTA TGCAGCCCTCAGAGCAAGCAAGACACTGACACCTTCAGGGGACTTGCAGGAGACCTTCAGTGGGATGGAT CAGGTGAAGGTGATGAAAAGAATTGCAGAGATGACAGACATCAAGCCCATCCTGAGAAAACTGCCCCGGA TCAAGAAGTATCTACTAAACTGTGACAACATGAGATGCTCAGTGAATGCCACCCCTCAGCAGATGCCTCA GGCAGAAAAAGAGGTGGAAAACTTCCTTAGAAATGTTGGCCGAAGCAAAAAGGAACGGAAGCCTGTCCGC CCGCATATTGTCGAGAAACCCACACCCAGTGGCCCCAGTGGAGCTGCACATGTCAGTGGGTCCCAGATCG TCAGAAAATTGGTGACAGACCCCACCTTCAAACCCTGCCAGATGAAGACACATTTTGTGCTGCCCTTCCC TGTGAATTACATTGGCGAGTGTGTCAGGACTGTCCCGTATGCTGATCCAGACCATGCCAGCCTTAAGATC CTTGCCCGTCTAATGACAGCTAAATTCTTGCATACGGAAATTCGAGAGAAGGGGGGTGCTTATGGTGGCG GTGCTAAACTCACCCACAGTGGGATTTTCACGCTTTACTCTTACAGGGATCCCAATTCCATAGAAACACT CCAGTCTTTTGGGAAAGCTGTAGACTGGGCTAAGTCTGGAAAGTTCACACAGCAGGACATTGATGAAGCC AAGCTGTCTGTTTTCTCTACTGTGGATTCTCCTGTTGCTCCATCCGATAAAGGAATGGACCACTTCTTGT ATGGCCTCTCCGATGAGATGAAGCAGGCATACCGAGAACAGCTCTTTGCTGTCAACCACGACAAACTGAC CTCTGTGAGCCATAAATACCTTGGCATCGGGAAGAGCACACACGGCCTGGCTATCCTCGGACCAGAGAAC TCAAAAATTGCCAAAGACCCATCATGGATCATAAAATAATGAGTGCCACATATCTTTGAGTATGTGTAAG AAACCAGAGGCTCTTAACAGCTGAGCCTCTGAGCTAAATTTAATGCGGATGATCACAAGAGTTACTAGTT TCTTTGTTCGGAAAATCAGTTAGCCATATAAAAACCAACCAAAGGTGTTTATTGACTGGCAAAAACTCTG AAGGAAATTCTGAGACCATGAAGAAATCATTAATCATGCATTAAATGACAGCAGTGAGCAAATTAAGCCT CTGAAACATTACTAAGCCTAGAATATGTATTTTAAATATAAAAGCCAACTCCAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3601081 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3601081 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11621 same embryo
	EMAGE:11620 same embryo
	EMAGE:11619 same embryo
	EurExpress:euxassay_006516 same experiment
	MGI:4827223 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source