EMAGE mouse gene expression spatial database

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EMAGE:11624

Pld2 phospholipase D2 ( MGI:892877)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006698. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006698_01	euxassay_006698_02	euxassay_006698_03	euxassay_006698_04

euxassay_006698_05	euxassay_006698_06	euxassay_006698_07	euxassay_006698_08	euxassay_006698_09

euxassay_006698_10	euxassay_006698_11	euxassay_006698_12	euxassay_006698_13	euxassay_006698_14

euxassay_006698_15	euxassay_006698_16	euxassay_006698_17	euxassay_006698_18	euxassay_006698_19

euxassay_006698_20	euxassay_006698_21	euxassay_006698_22	euxassay_006698_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11624_wholemount_strong.wlz

11624_wholemount_moderate.wlz

11624_wholemount_weak.wlz

11624_wholemount_possible.wlz

11624_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11624_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hypothalamus ventricular layer

strong

regional

strong expression: see section 15

diencephalon lateral wall ventricular layer

strong

regional

strong expression: see section 12 13 14 15

telencephalon ventricular layer

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 16 17 18 19 20 21

medulla oblongata basal plate ventricular layer

strong

regional

strong expression: see section 13 14 15 16 17 moderate expression: see section 12

pons ventricular layer

strong

regional

strong expression: see section 13 14 15 16 17 18 moderate expression: see section 08 09 10 11 12 19 20

midbrain ventricular layer

strong

regional

strong expression: see section 10 11 12 13 14 15 16 17 18

spinal cord ventricular layer

strong

regional

strong expression: see section 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8125
Entity Detected:	Pld2, phospholipase D2 ( MGI:892877)
Sequence:	sense strand is shown >T8125 GGGTGCGGGGGCCCGGGACGTGGCGCGGCTGAGGTCCGGAGCCCCGGGAACCGGGCGGGGACGCTGTCGG GCTGGAGCTGCAACGGCCTTCTGACTCAGCCGCTCGTCCAGATTTCGGGATCTCTCTCCTGGGCGGGGAG CGCGCAACTTCCTCATTCCCGCCCGGACCCTTGCTTCCAGCCAGTCTGACCTGACTTCGAGCCTCGCTGT CACCAGGGCTCCTCCCTTGGACTTAACCTCGCCCCAGTTCGAGGCCATCCCACCTTAGCTAGGATGACTG TAACCCAGAAGAACCTCTTTCCCTATGGGGACTATCTGAACTCCAGCCAGTTGCACATGGAGCCAGATGA GGTTGACACTCTGAGGGAAGGAGAGGATCCAGCTGATCGAATGCATCCCTATCTGGCCATCTATGACCTT CAGCCTCTGAAAGCACACCCCTTGGTGTTCGCCCCTGGGGTCCCTGTTATAGCCCAGGTGGTGGGCACCG AAAGATACACCAGCGGATCCAAGGTGGGAACCTGTACTCTATATTCTGTTCGCTTGACGCATGGTGACTT TACCTGGACAACCAAGAAGAAGTTCCGACACTTTCAGGAGCTGCATCGGGACCTCCAGAGACACAAAGTC TTGATGAGTCTGCTCCCTTTGGCTCGCTTTGCTGTGACCCATTCTCCAGCCCGAGAGGCAGCCGCCGAGG ATATACCCTCCCTACCCCGAGGAGGTTCTGAGGGCTCTGCCAGACACACAGCCAGCAAACAGAAATACTT GGAAAATTACCTCAACCGCCTCCTGACCATGTCTTTCTATCGCAATTACCACGCCATGACAGAATTTCTG GAAGTCAGTCAACTTTCCTTTATCCCAGACCTTGGCTCCAAAGGACTGGAAGGGGTGATCCGGAAGCGCT CGGGCGGGCATCGAGTTCCCGGCTTCACCTTCTGTGGCCGAGACCAAGTTTGTTATCGATGGTCCAAGAG GTGGCTGGTGGTGAAGGACTCCTTCCTGCTGTACATGCGCCCGGAGACCGGCGCCATCTCATTTGTTCAG CTTTTTGACCCTGGCTTTGAGGTCCAGGTCGGAAAAAGGAGCACAGAGACGCGGTATGGGGTGAGGATCG ACACCTCCCACAGGTCCCTGATTCTCAAATGCAGCAGCTACCGGCAGGCACGGTGGTGGGGCCAGGAGAT CACGGAGCTGGCACAGGGTTCGGGCAGAGATTTTCTACAGCTACATCAGCATGACAGCTATGCCCCACCC CGGCCCGGCACCCTGGCCCGGTGGTTTGTGAATGGGGCAGGTTACTTTGCTGCTGTGGCAGATGCCATCC TGCGAGCTCAAGAGGAGATTTTCATCACAGACTGGTGGTTGAGTCCTGAAATTTACCTGAAGCGTCCAGC CCATTCCGACGACTGGAGACTGGACATTATGCTCAAGAGGAAGGCGGAAGAAGGTGTCCGAGTTTCCATA CTGCTGTTTAAGGAAGTGGAGCTGGCCTTGGGCATCAACAGTGGCTACAGCAAGAGGACGCTGATGCTGC TGCATCCCAACATAAAGGTGATGCGACACCCAGACCTTGTGACACTGTGGGCTCATCACGAGAAGCTCCT GGTGGTAGACCAAGTGGTGGCATTCTTGGGCGGGCTGGACCTGGCCTTCGGCCGCTGGGATGACGTGCAA TACCGACTGACTGACCTGGGTGACCCCTCTGAACCTGTACATTTACAGACTCCCACACTAGGTTCAGACC CTGCAGCCACTCCAGACCTCTCGCATAACCAATTCTTCTGGCTGGGAAAGGACTACAGCAACCTCATCAC CAAGGACTGGGTGCAGCTGGACCGGCCTTTTGAAGATTTCATCGACAGGGAGACCACACCCAGGATGCCA TGGAGGGATGTTGGAGTGGTTGTACACGGAGTAGCTGCCAGGGACCTTGCCCGGCACTTCATCCAGCGCT GGAATTTCACCAAGACCACCAAGGCCAGGTATAAGACACCTTTGTACCCCTACCTGCTGCCCAAGTCCAC CAGCACTGCAAACAATCTCCCCTTCATGATCCCAGGCGGGCAGTGTGCCACTGTGCAGGTCTTGAGGTCT GTGGATCGATGGTCAGCAGGGACATTGGAGAACTCCATCCTCAATGCCTACCTACATACCATTCGAGAGA GCCAGCACTTTCTCTACATTGAGAATCAGTTCTTCATTAGCTGCTCAGATGGGCGAACAGTTCTGAACAA GGTGGGCGATGAGATTGTGGACAGAATCCTGAAGGCTCACGAACAGGGGCAGTGTTTCCGAGTCTACTTG CTTCTGCCTTTGCTCCCTGGCTTTGAGGGGGACATCTCCACAGGGGGTGGTAACTCCATCCAGGCTATTC TGCACTTCACCTACAGCCTCTGCCTCCTTCACCCTTTCTTCTCTCTCAGGACCCTGTGTCGTGGGGAACA TTCAATCCTACATCGTCTCAAAGCAGCCATGGGGACTGCGTGGCGAGATTACATGTCCATCTGTGGGCTT CGCACCCATGGAGAGCTGGGCGGGCACCCAATCTCTGAGCTCATCTATATCCACAGCAAGATGCTCATTG CGGATGACAGAACAGTCATCATTGGTTCTGCGAACATCAATGACAGGAGCTTGCTGGGGAAGCGTGACAG TGAGCTAGCCATCCTGATCAAGGACACAGAAATGGAACCATCCCTCATGGATGGGGTGGAGTACCAGGCA GGCAGATTTGCCTTGAGTTTGCGGAAGCACTGTTTCAGTGTGATTCTTGGGGCAAATACCTGGCCAGACC TGGATCTCCGAGACCCTGTCTGTGATGACTTCTTCCAGCTGTGGCAAGAAACAGCGGAGAACAATGCCAC CATCTATGAGCAGATCTTCCGCTGCCTGCCGTCCAATGCTACCCGTTCCCTGCGGGCTCTCCGGGAGTAT GTGGCTGTGGAGTCCTTGGCTACAGTCAGCCCTTCTTTGGCTCAGTCTGAGCTTGCCCACATCCAGGGCC ACCTAGTTCACTTCCCCCTCAAGTTTCTGGAGGACGAGTCCTTGTTGCCCCCACTGGGGAGTAAAGAAGG GATGATACCTTTAGAAGTGTGGACATAGCTGAGGCTTGCGCTCAGCGGCAGCCGCGAGCCGTTGGGCCCT ATCGTGCCTGGATCCTTGCCCCAAACCCTGAGTTCTGAGGGCAGTGCCCTTCGATCCTTTGGGAGGACAT CTCTGAGGACTCCTAGAGAACCACAGAGGACTTGTACTTGTGAAGTAGCCAAAGGAAGCACTCCCAAGTC TGGCCTGGGGAGGCAGGAGAGAGTTGTAGAGAGGTTTGCCTTCCTGTCACCAAGTTCAAACCACTACGCC ATAGAACCCTAGCCCTACGAGGCAGCCTGAGGACAAGCCTTTTTATTCCAGGAAAAGGGACTCCTGTCTT GGGTCTTCTTCATCTCACTCTTCCTCCTGCCCTTGAACCCCCTACATGTCCCAGGGCCTCTCCCAGCCCA CTGCTGCAAAGATGGAGGGAGTATGGAGCCACTTTGACCACTGTCTCCACCAATGGTGGTGGGAAAGACA CCAGAACTGCCTCCGCCAGCCTGCTGACAGACACTAACTTTGTACTGGTTCATCAAGCATTTCATAAATA AAAGTGTAGAAACGGAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30094606 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30094606 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11623 same embryo
	EMAGE:11622 same embryo
	EurExpress:euxassay_006698 same experiment
	MGI:4827259 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source