EMAGE mouse gene expression spatial database

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EMAGE:11638

Pcdhb15 protocadherin beta 15 ( MGI:2136750)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006692. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006692_01	euxassay_006692_02	euxassay_006692_03	euxassay_006692_04

euxassay_006692_05	euxassay_006692_06	euxassay_006692_07	euxassay_006692_08	euxassay_006692_09

euxassay_006692_10	euxassay_006692_11	euxassay_006692_12	euxassay_006692_13	euxassay_006692_14

euxassay_006692_15	euxassay_006692_16	euxassay_006692_17	euxassay_006692_18	euxassay_006692_19

euxassay_006692_20	euxassay_006692_21	euxassay_006692_22	euxassay_006692_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11638_wholemount_strong.wlz

11638_wholemount_moderate.wlz

11638_wholemount_weak.wlz

11638_wholemount_possible.wlz

11638_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11638_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8120
Entity Detected:	Pcdhb15, protocadherin beta 15 ( MGI:2136750)
Sequence:	sense strand is shown >T8120 AGCCATAGGAAATGATATAGCTAGCGGTAGCAAGCCCTGGAGAAGGCACCCCTCTCTTCTCACAGGAATT GCTAGTGTCCAGGTCATTCCTAAGGATTCCAGCTGCAGGCATTCCAGGGAGCTGCTTGAGGCTAAATGGA AGGAATTTGGGGTTTTATTTTGAAAGTCTTGGAAAGAATGGAGGCCAGATTGGAACAAGCTGTGCAGAAA AGGCAAGTACTGTTTCTTTATGTGTTTCTTGGAGCGTCTTGGGCTAGCGCCGAACAGCTTCAGTATTCTG TGGCAGAGGAAACCGAGAGAGGGACTTTCCTAGCCAATCTAGCAAAGGATTTGGGGCTGGGGCTGGGGGA ACTGTCAGCCCGGGAAGCTAGAATTGTTTCAGACCAGAACACACGATTTTTACTGCTCAGTCCGCTTACT GGAGAATTAATTCTAAATGAGAAGCTGGACCGAGAGGAACTGTGCGGCCCCACAGAGCCCTGTGTGCTGA CTTTCCAGTTGCTGTTGGAAAGACCATTTCAGAGTTATCGCGCTGCACTACATATCAAAGATATAAATGA TAATTCTCCAGTATTCCTAGACAAAGAAATACTCTTGAAAATATCAGAAAGTACCACTCCAGGGGCGACG CTTCTCTTGGAAAGGGCACAGGATGCCGATGTGGGAACGAACAGCCTGAGTAACTACACCATCAGCCCCA ATGATTATTTCCATATTCACGTGCATGATGGAGGGGAGGGGCCTATCATTCCTGAACTGGTACTGGATAA AATGCTGGACCGGGAAGAAATACCAGAATTCACTTTAACTCTCACAGCCTTAGATGGTGGCTCTCCACCC AGATCTGGGACAGCCTTGGTGCAAATCTTGGTGTTGGACATAAATGACAACTCCCCTCAATTCGTGCAGT CACTTTACAAAGTTCAGTCACCTGAAAACACCCCTGTTGGCTCCTTGGTTGTCGCTGTGTCTGCCAGGGA CTTAGACACAGGAAGTTACGGACAAATAGACTACACATTTTTTTATGCCACAGAAAGAATTCTCCAAACG TTTCAAATCAATTCTACATCTGGCGAACTTTATCTTAAACGCGAACTCAACTATGAAGCGATTCAAACTT ATTCGATAACTATTCAGGCCAGAGATGGTGGTGGACTTTCTGGGAAATGTGCTGTGGTTGTAGAGGTAAT GGACGTGAATGATAACCCACCTGAATTTCTCTTGTCGTCACTTAATAGCCCAATTCCAGAAAATTCACAA GAGACAGTTGTTGCTGTATTTAAAATTAGAGACAGAGATTCGGGGAACAATGGAAAAACCCTGTGCTCCA TTGCGAATGATCTCCCTTTCGTCCTGAAGCCATCTGTGGAAAATTTCTATACTCTGGTAACAGAGAAGCC TTTAGATAGAGAATCAAACACTGAATACAACATCACCATCACAGTCACCGACATGGGCATACCCAGGCTC ACAACCCAGCACACCATAACAGTGCAGGTCTCTGACATCAACGACAACGCCCCCACCTTCACCCAAACCT CCTACACCATGTTCGTCTGCGAGAACAACAGCCCCGCCCTGCACATTGGCACCATCAGCGCCACAGACTC AGACTCAGGCTCCAATGCCCACATCACCTACTCGTTGCGGCTGCCTCACGACCCGCAGCTGGCCCTGGAC TCGCTCATCTCCATCAATGCAGACAATGGGCAACTGTTCGCGCTCAGGGCTCTGGACTATGAGGCCCTGC AGGCCTTCGAGTTCCACGTGGGTGCAATAGACCAAGGCTCCCCTGCGCTCAGCAGTCAGGCTCTGGTGCG CGTGGTGATACTGGACGACAATGACAATGCGCCCTTCGTGCTCTACCCGATGCAGAACGCCTCTGCACCC TACACTGAGCTGCTGCCCAGGGCGGCAGAACCCGGATACCTGGTCACCAAGGTGGTGGCAGTTGACCGTG ATTCTGGCCAGAACGCCTGGCTGTCATTTCAGTTGCTTAAGGCCACAGAGCCCGGACTGTTTAGTGTGTG GGCGCACAATGGTGAGGTGCGCACCACCAGGCTACTAAGCGAGCGAGATGTGCCCAAGCATAAGCTGCTA GTGGTGGTCAAGGATAATGGAGAGCCTCCACGCTCTGCCAGTGTCACACTGCATGTGTTGCTGGTCGATG GCTTCTCTCAGCCCTTCCTGATTCTGCCAGAGGTGGCGCGCGACCCTGCACAAGAAGATGATGAGCTAAC ACTCTACCTGGTCATTGCCTTGGCTTCTGTGTCTTCTCTCTTCCTGGTATCTGTGCTGCTGTTCGTTGGA GTAAGACTCTGCAGGAGGGCCCGGGCAGCCTCTCCGGGTGTCTGCTTTGTGCCTGAAGAACACTTTCCTG GCCACCTGGTGGATGTCAGCGGTGCAGGGACCCTGTCCCAGAACTACCAGTATGAGGTGTGTCTGACTGG AGGATCAGGGATAACCAGTGAATTTAAGTTTCTAAGTCCAATTGCCTCTAACTTCCTAACCGAAAGCACA GGGAGAGAAATAGAATAAAAGCTAAACCCTTCTGCCCATGGATGAAGCTCTAATGTAAACTATACCCTTG TTGTGTAATGAAGAATGATTTCATGTTAACATCTAGAAGTTAACTAATTTAGGACAGAAAAGAATACCTG TATTTATTCCCAAAAACCTTTTCTTTAATTTATGAAATGCATGTGTTGTGTGCTATCTCAAATCTAATTT GATTTCTCTTTGTTTTTTAAGATTTGTTGAACTGCAATCTATTAAACAACAAATCTTGGTAAAAAAAAAA AAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30100073 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30100073 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11634 same embryo
	EMAGE:11636 same embryo
	EMAGE:11637 same embryo
	EMAGE:11635 same embryo
	EMAGE:11633 same embryo
	EurExpress:euxassay_006692 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source