EMAGE mouse gene expression spatial database

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EMAGE:11669

Nhedc2 Na+/H+ exchanger domain containing 2 ( MGI:2140077)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006992. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006992_01	euxassay_006992_02	euxassay_006992_03	euxassay_006992_04

euxassay_006992_05	euxassay_006992_06	euxassay_006992_07	euxassay_006992_08	euxassay_006992_09

euxassay_006992_10	euxassay_006992_11	euxassay_006992_12	euxassay_006992_13	euxassay_006992_14

euxassay_006992_15	euxassay_006992_16	euxassay_006992_17	euxassay_006992_18	euxassay_006992_19

euxassay_006992_20	euxassay_006992_21	euxassay_006992_22	euxassay_006992_23	euxassay_006992_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11669_wholemount_strong.wlz

11669_wholemount_moderate.wlz

11669_wholemount_weak.wlz

11669_wholemount_possible.wlz

11669_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11669_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

femur

strong

spotted

strong expression: see section 05 06 07 23 24

stomach

moderate

regional

moderate expression: see section 06 07 08 09 10

mandible

strong

spotted

strong expression: see section 05 06 07 08 09 10 11 12 13 18 19 20 21 22 23 24

lower jaw incisor

moderate

regional

moderate expression: see section 13 14 17 18 19

upper lip

strong

regional

strong expression: see section 13 14 15 17 19 20 moderate expression: see section 16 18

maxilla

strong

spotted

strong expression: see section 07 08 09 10 11 12 13 19 20 21 22 23 24

upper jaw incisor

moderate

regional

moderate expression: see section 13 14 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9991
Entity Detected:	Nhedc2, Na+/H+ exchanger domain containing 2 ( MGI:2140077)
Sequence:	sense strand is shown >T9991 AAGCCAGGAGGCCTGCGCAGACTGCCAGCAATGAGAGCAGCTCTGGCTCCTCATCCCTGAGTCATCATCT TCGAAATTATAAGACATGGAGGATGAAGATAAGACAGCTGAATGTCAGCATTCAAAGCCACCTACGGGGA TCACACACGAGGCTCCTCCACATCACGAACTACGGGAAGAGAGAGTCATGAGCCTCAGAGGTACAGACAG AAGTGAACCGACAGAAGGCAGTAACCTGCTGACCAGCGGTGAAAAAAAGCCACAGGACTCACCAACAGAA CCCAATGGCCTGCAGAGTCTGAGGCGATTCCTGGCCTGCCCTCCACGAGGCTGCCTGGCAAGAGTGATAA CGAACGGTACCATGGTTGTTCTTCTGTGGGCCATGGTTTGGTCAGTTACCGGCCCTGAATGTCTTCCTGG AGGAAATCTGTTTGGAATTATCATTCTGTTCTATTGTTCCATCACCGGAGGTAAACTTTTTGGACTCATT AAGTTTCCAACATTGCCTCCTCTGCCTCCTCTTCTTGGCATGCTGCTAGCTGGGTTTCTCTTGAGGAATA TCCCAGTCATCAATGATAGCGTCCGGATCCAACACAAGTGGTCGTCATCTTTGAGAAGCATAGCCCTTTC TGTCATTCTGGTTCGTGCTGGCCTTGGTCTAGATTCAAAGGCCCTGAGGAAGCTGAAGGGTGTGTGTGTG CGACTGGCCATGGGTCCCTGCATCGTGGAGGCGTGTGCTTCTGCGATTCTCTCACACTTCCTGATGGGGT TGCCATGGCAATGGGGGTTCATCCTGGGTTTTGTCGTAGGTGCCGTGTCCCCAGCTGTCGTGGTGCCCTC CATGCTCCTTTTGCAGGAAGGAGGCTACGGTGTTGGAAAAGGTATCCCAACCTTACTCATGGCCGCCGGC AGCTTCGATGACATCCTGGCCATCACTGGCTTCAACACGTGCTTAGGCGTGGCCTTTTCCACAGGATCTA CAGTTTTTAACATCTTCAGAGGCATCTTGGAGGTGGTAATTGGTGTGGCAGCTGGATCTTTTCTTGGGTT TTTTATCCAGTACTTCCCGAGCAGGGACCAGGACAACCTCGTGTGGAAGCGAGCCTTTCTGGTTCTGGGT TTTGCTGTGCTCGCTGTGTTCAGCAGTGTGTATTTTAGCTTCCCGGGGTCTGGAGGACTCTGCACGTTGG TCATGGCTTTCCTAGCAGGCATGAGGTGGACTGACAAGAAGTCAGAGGTAGAAAAGGTCATTGCAGTTAC CTGGGACGTTTTCCAGCCTCTTCTTTTTGGCCTGATTGGAGCAGAGGTTTCCATTGTGTCTCTCAGAGCA GAAACGGTTGGCCTTTGTGTTGCAACCCTCAGCATCGCAGTGCTTATACGAATTCTGACTACATTCCTGA TGGTGTGTTTCGCTGGCTTTAACATAAAGGAAAAGATATTTATTTCTTTTGCCTGGCTTCCAAAGGCCAC GGTCCAGGCTGCCATTGGCTCTGTGGCTCTGGACACGGCAAGATCCCACGGAGAGAAGCAGCTGGAAGAC TATGGGATGGATGTGCTGACGGTGGCATTTTTGGCCATCCTCATTACAGCACCAATTGGAAGCCTACTGA TTGGTTTGCTGGGTCCCAGGGTTCTTCAGAAATCTGAACATCGAACCGAAGAGGAGGTTCAAGGAGAGAC TTCTGCACACATTCAGAGGAAGCCTGAGGATTCCATTACGGAAGCCTGATGGACCATGTTTACCATCCCA ACCCAAAGGTTTTGGCCCTCCAACAACCGGGACAACTTTACTTCCCTTTGACTCAGAAGAAAACTTCCCG TGGAATTTCATAAGCAAACAAATTAGAAAGCTTTACGCTGCTAACAGTACCTCAGGTGTTTACTTCCTCA GAAAGACCGGAGGACAGGTTACTTCAGAAAGTGAGAGAAAGTAATTTGGACAAATAAAACATTCACGATT TTGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30503721 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30503721 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11666 same embryo
	EMAGE:11668 same embryo
	EMAGE:11665 same embryo
	EMAGE:11670 same embryo
	EMAGE:11667 same embryo
	EurExpress:euxassay_006992 same experiment
	MGI:4826703 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source