EMAGE mouse gene expression spatial database

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EMAGE:11678

Zc3h12a zinc finger CCCH type containing 12A ( MGI:2385891)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006808. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006808_01	euxassay_006808_02	euxassay_006808_03	euxassay_006808_04

euxassay_006808_05	euxassay_006808_06	euxassay_006808_07	euxassay_006808_08	euxassay_006808_09

euxassay_006808_10	euxassay_006808_11	euxassay_006808_12	euxassay_006808_13	euxassay_006808_14

euxassay_006808_15	euxassay_006808_16	euxassay_006808_17	euxassay_006808_18	euxassay_006808_19

euxassay_006808_20	euxassay_006808_21	euxassay_006808_22	euxassay_006808_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11678_wholemount_strong.wlz

11678_wholemount_moderate.wlz

11678_wholemount_weak.wlz

11678_wholemount_possible.wlz

11678_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11678_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8180
Entity Detected:	Zc3h12a, zinc finger CCCH type containing 12A ( MGI:2385891)
Sequence:	sense strand is shown >T8180 GCTACGAAGGTGCCGTCAGAGCCCGAGACTCGGCTCCATGGACACCTTACAGACGACGTCCGGCGCTCAG CGGGGAGCTGTGATGGACACATTCCAAGAATGTGAAATATGAGTGACCCTTGTGGAACGAAGCCTGTCCA AGAATCCAACCCCACCATGAGTCTGTGGAGTCTTGAGGACAGACACAGCAGCCAGGGTCGACCTCAGCCA GACCAGGATCCTGTGGCTAAAGAGGCCCCTACTTCCGAGCTGCAGATGAAGGTGGACTTTTTCCGTAAAC TGGGGTACTCGTCCTCTGAGATCCACAGTGTCCTGCAGAAGCTGGGAGTCCAAGCAGACACCAACACGGT GCTAGGGGAATTGGTGAAGCATGGCTCAGCTACTGAACGAGAATGCCAGGCCCTGACGGCCCCCAGCCCC CAGCCCCCTCTGGTGCCCCGGGGTGGAAGCACCCCCAAGCCTTCCACTCTAGAACCCTCACTCCCAGAGG AGGACAGAGAGGGCAGCGACCTGAGACCTGTGGTCATCGACGGAAGCAATGTGGCCATGAGCCATGGGAA CAAGGAAGTCTTCTCTTGCCGGGGCATTCTGCTGGCTGTGAACTGGTTTCTGGAGCGAGGCCACACAGAT ATTACCGTGTTTGTGCCATCTTGGAGGAAGGAACAGCCTCGACCAGATGTGCCTATCACAGACCAGCACA TCCTTCGGGAACTAGAGAAAAAGAAGATCTTGGTGTTCACGCCATCCAGGCGGGTCGGCGGCAAGCGCGT GGTGTGCTATGATGACCGCTTCATTGTGAAGCTGGCCTTCGAATCCGACGGAGTGGTGGTCTCCAATGAC ACGTACCGAGACCTCCAAGGCGAGAGGCAGGAGTGGAAACGCTTCATCGAGGAGCGGCTGCTCATGTACT CCTTCGTCAATGACAAGTTCATGCCCCCTGACGACCCTTTAGGACGGCATGGGCCTAGCCTGGACAACTT CCTTCGTAAGAAACCACTGCCTTCTGAGCACAGGAAGCAGCCATGCCCCTATGGGAAGAAATGTACGTAT GGAATCAAGTGCCGATTTTTCCACCCTGAGCGGCCAAGCCGTCCCCAGCGCTCTGTGGCCGATGAGCTCC GTGCCAACGCTCTCCTCTCACCTCCCAGGACTCCAGTCAAGGACAAAAGTAGCCAGAGGCCTTCCCCTGC CTCTCAGTCCAGCTCTGTGTCCCTAGAGGCTGAACCAGGCAGCCTGGATGGGAAAAAGCTGGGTGCCAGG TCATCTCCGGGTCCCCACCGAGAAGGCTCACCGCAGACCTGTGCTCCAGCTGGCAGGAGCCTCCCTGTTA GTGGGGGCAGCTTTGGGCCCACAGAGTGGCTTGCACACACCCAGGACTCACTCCCATACACCTCCCAGGA GTGCCTTGATTCAGGCATTGGTTCCCTGGAGAGCCAGATGTCAGAATTATGGGGCGTGCGAGGAGGCAGC CCTGGGGAGTCGGGCCCCACTCGGGGCCCCTATGCAGGTTATCACAGCTATGGATCCAAGGTCCCAGCAG CACCTTCCTTTTCTCCTTTTAGACCAGCCATGGGTGCTGGCCACTTCAGTGTCCCCACCGACTATGTGCC CCCGCCACCCACCTACCCATCCAGAGAGTACTGGTCTGAGCCGTACCCATTACCCCCACCCACTCCTGTC CTTCAGGAGCCCCAGAGACCCAGCCCCGGGGCTGGTGGGGGCCCCTGGGGCAGGGTGGGTGACCTGGCCA AAGAAAGGGCTGGTGTATATACCAAGCTGTGTGGTGTCTTCCCCCCACACCTGGTAGAAGCTGTAATGAG ACGCTTCCCACAGCTGCTGGATCCGCAGCAGCTGGCCGCAGAGATCCTGTCTTACAAGTCCCAGCACCTC AGTGAGTAAGCCAGAAGGTGGCGCAAGGGGCTGTCGGGCATCACAGATAGCGGTCCCCAGCCCCTGCCTG GCCTACCCTGGAAGCTGGACAGAAGGACAAGTGAAGCAGGCAAGGGAATCCTCAAACCAAAGATACCATA GGATTGGTTCTGGCCCCGCGGCACCCCAACCCGTCCCGCACAGGTCAGAAGTGATCACCCTGTTGATACA CATTGTATCTCTGTCATTTAAGGAGACGCTGCCGGTCAGGCCGTCCATCCGTGGCTGATGCCCAAACCCT CTTTTTTTTTCTCAGAGGGCCGGGTGGGAGGCATGGGGGAGCAGAGGCCTGAGCTGGACCCCACCTTCCA CCCTGTCCCTGGGACGCCGGCACCACCGGCTAGTTAGGCACCATGTGCCTGCTCTCTGAGGCCCCCTCAA GCCAATGCGGCCTCATCCCTGTTCACAGGGCATGAGGCTTCATGTTAGTAAGCAAGATGCTTCTTTAAGC CCCTCCCCTGCCCGCTCTGTCCACCTACACACCCCCCCCCCAACCAGGGCTCCAAGGCCCTCTGTTTCCA CACCTCCCATGGGTGGGAGGACACATGTATGCTGTGTACAGAGGCGAGATTTAAATATTTTAAATGAAAA AGGTTGACAAAATAAAGGCTATTGCCAGGCAGGCTGGAGAGATGGCTCAGTGGTTAAGAGCACCGACTGC TCTTCTGAAGGTCCTGAGTTCAAATGTCAGCAACCACATGGTGGCTCACAAACATCTGTGATGAGATCTG GTGCCCTCTTCTGGGGTGTCTGAAGACAGCTACAGTGTACTTACATACAATAATAAATCTTTTAAAAAAG AGAGAAATTTAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5254681 using vector (pT7T3D-PacI) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5254681 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11677 same embryo
	EMAGE:11679 same embryo
	EMAGE:11676 same embryo
	EMAGE:11675 same embryo
	EurExpress:euxassay_006808 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source