EMAGE mouse gene expression spatial database

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EMAGE:11705

Mrgpre MAS-related GPR, member E ( MGI:2441884)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006868. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006868_01	euxassay_006868_02	euxassay_006868_03	euxassay_006868_04

euxassay_006868_05	euxassay_006868_06	euxassay_006868_07	euxassay_006868_08	euxassay_006868_09

euxassay_006868_10	euxassay_006868_11	euxassay_006868_12	euxassay_006868_13	euxassay_006868_14

euxassay_006868_15	euxassay_006868_16	euxassay_006868_17	euxassay_006868_18	euxassay_006868_19

euxassay_006868_20	euxassay_006868_21	euxassay_006868_22	euxassay_006868_23	euxassay_006868_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11705_wholemount_strong.wlz

11705_wholemount_moderate.wlz

11705_wholemount_weak.wlz

11705_wholemount_possible.wlz

11705_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11705_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 09 10 17 weak expression: see section 16

facial vii ganglion

moderate

regional

moderate expression: see section 04 05 23

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 08 19 20

trigeminal v ganglion

moderate

regional

moderate expression: see section 04 05 06 07 09 10 21 22 23 24 weak expression: see section 08 19 20

cervico-thoracic ganglion

moderate

regional

moderate expression: see section 09 10 16

cervical ganglion

moderate

regional

moderate expression: see section 09 17

thoracic ganglion

moderate

regional

moderate expression: see section 12 13 14

dorsal root ganglion

moderate

regional

moderate expression: see section 07 08 09 10 11 12 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8146
Entity Detected:	Mrgpre, MAS-related GPR, member E ( MGI:2441884)
Sequence:	sense strand is shown >T8146 CTGCCTGCAGCCCGGCAGCTCCGGCAGCAGCTGGAGCCTCCGCCTGGCCAGGAATTTCCTGGCTAGTTTC TCCAAGACCCGGGGTGGAGCAGGGAGAAGCAGAACGGGGTGTCCGGAGAGAGAAAACGCAACACAGACTT CTGCAGACATCAGCCATGACGTCCCTGAGCGTGCACACAGATTCTCCCAGCACCCAGGGAGAAATGGCTT TCAACCTGACCATCCTGTCCCTCACAGAGCTCCTCAGCCTGGGCGGGCTGCTGGGCAATGGAGTGGCCCT CTGGCTGCTCAACCAAAATGTCTACAGGAACCCCTTCTCCATCTATCTCTTGGATGTGGCCTGCGCCGAC CTCATCTTCCTCTGCTGCCACATGGTGGCCATCATCCCTGAGCTGCTGCAGGACCAGCTGAACTTCCCTG AATTTGTACATATCAGCCTGACCATGCTGCGGTTCTTCTGCTACATTGTGGGCCTGAGCCTCCTGGCGGC CATCAGCACGGAGCAGTGCCTGGCCACTCTCTTCCCTGCCTGGTACCTGTGCCGCCGCCCACGCTACCTG ACCACCTGTGTGTGTGCGCTCATCTGGGTGCTCTGCCTGCTACTGGACCTGCTGCTGAGCGGCGCCTGCA CCCAGTTCTTTGGAGCACCCAGCTACCACCTGTGTGACATGCTGTGGCTGGTGGTGGCAGTTCTCCTGGC TGCCCTGTGCTGCACCATGTGTGTGACCAGCCTGCTCCTGCTGCTGCGGGTGGAGCGTGGTCCAGAGAGA CACCAGCCTCGGGGCTTCCCCACCCTGGTCCTGCTGGCCGTCCTGCTCTTCCTCTTCTGCGGCCTGCCCT TTGGCATCTTCTGGCTGTCCAAGAACCTGTCCTGGCACATCCCCCTCTACTTCTATCATTTCAGCTTCTT CATGGCCAGTGTGCACAGTGCAGCCAAGCCTGCCATCTACTTTTTCTTGGGCAGCACACCTGGCCAGAGG TTTCGGGAACCCCTCCGGCTGGTGCTCCAGCGGGCACTTGGAGATGAGGCTGAGCTGGGAGCTGGGAGAG AGGCTTCCCAAGGGGGACTTGTGGACATGACTGTCTAAGCACAGTGGGTCACAACTGCAGCTTCAGCCCA TGGGGGTCCAGGGGAGCTGCCTGATGTAGGTAAAGCTGGGATCAGAGCTCCATCAGTAAGACTCTTGAGG GACATCTTTGCTGATGACCCAGTGCTGTGTCCCCTGGGAGGATTCTGGGAAGGGGCAAGCAGAGAGTGAT GCTTCTGTGGAGGGCCTGGGGTTGTGTGTGTTAGGCAGAGCTCCTGGACCACCTCATAACAGCTGCTTCC ATGGACACCCTCCCTCCCCACATGGCTACAGTGAGGGGACAGAGCAGGCATGAGACAAGCTAGTCCTGGT GGGGGGCAACTGTTCTTCTACCACTAGCATCCTCCTAGCTAGAGTGTGCAGTGACTTTTCTCGGTGCCCA ATGCTCCTTCCTTTAGTGAGGGATAAATGACACTGAAACTGGACATTCAGTTAGGTATGGGGAAATAAGT GGCTTGGAAGTTCTCAGCGGCTGAACTTGGAAGTCCTAAGTGGCAGAAGGCATCCTCCTGACCTCACATC ACTGCCAGAAGGCAATGCTGGCACAGGCCTGTCATCCCAGCTACTTGAGAGACTGGGGCAGGATTGTAGC CCCAGACTGGGATTTATCTGTAACTCTAGTTCCAGGGGATCTGTCTCCGTCTTTTGGCTTCTGCAGACAC TGCATGCATGTGGTACACATACATACATACATACATACATACATACATGCATGCATGCATGCATGCATAC ATACATACATACATGTGTGTGACTCCAAATACGCGCACGCGCACACGCGCGCGCGCGCACACACACACAC ACACACACACACACCACACACACACACACACACACACACACACACCACACACACACACACACACACACAC ACACACACACACAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAATAGAATTCAAGGTCAGATTATG CTTTAGAGGACTCATCATCTCAGGAAACCAAGCCCAGAGGGAACTGTGTTGAGGAGAGAGACCACTGGAG GATATAAACCTCATCCAATGTTTCAGAAAAACTGGTGGCTCCTGAGTCTGAATTTCAAAGTGAGGTCCTC TGCATGGGCAGCAAGCCCCCTCCTGTCGCTGTCCATATCCAAAGCACCTCTACCTAGCAGGAGAGAGCCC AGGGCTTCAGCACCAGGACAGTTCCTACCTTATCCTCTTTTCGGGATAGGACCTACCCACCTAGCAGGGG TTCTCTGAGGAAGCAGCCTGGTTGCAGGCCACCACAGGTGCCTGAGCTCCTGCCTAGCCAGGAGCACAGA AATGAAGGGGACCACCCCCCAATCCAGTCACAGGTGGCCGAGAACCAGGGCTTGGGAGCCCACCAGTCCT GAAAGGACTTACCATCATCCATGTATCATAGTCCTAACTCAGCCCTTACCTTTCTACTTCCTCACTGAAT AGTCAATTGATTACACATACGCACAGTGTTGTTGTGTCCAGCAAGTCCCAAGAGGATTTTATTACATCAT CAACCCTCCAGATCCACATTCGTGACATGCTGGTCTCCTTTGACATCTGTGTGGGTGTAATACTGACTTG GACCAGGCCCACAGATGGAGGGACATTTGATCTCTGTCATGACTAAACTTGCTCCTAAGTCTGGACACGG ACAGAAGAACCCTGCCTGGCATCCCGGAATTGGCAACTCACTGCCCCCACCCCACGCAGGTTGTGTTGGG ATGCATCCTGTGGTATGGTGCCTTCATCTCAAATTCTCTGGCAAGTCTATCTACATGCAGGGACCCCAGG AAGCTAGGAGCTTGGGAGTGCTGGTCTTCCTCTTCCAAAGGAGTTCTTTGTATCCCCCACAAAGGCCTTC CCTCCTCCACACTGGAGCTTAGGAGCCGATGTGGCTATCTGTCTGCCTCCAGGACAGGATTCCCAACTTC AGTTCCTCAGCTTGGGGAACTGAGAGCACGAAGGGAAGTAGCCTGTCCACAAGTCCAATTTTGGCCTCTG GACACCCACTTCTTCATGGGGGAAAGCCCATGCCAGCCTTACAGGTGTCTCCCCAACTGGGCACTAGCCC AGAGTCTTTGAGGCTCAGAGAAGCCCGTGTCTGCTCGAGACAACCCGGGACATGGCACAGACCTTCTACT CCTCTCCTAGCTAGCTTCAGCTGTTGACAGGGCATAGTCCAGAGTTATCTGAGGGAGTCTCAGCTAGGGA ACTGTTTGGGTCAGGTTGGCCTGTGTGCACTGTCCTGACTGCTAATACATGTGGGAGAGCTCACTCCACT GTGAGCAGAACCACCCGTAGGCAAATGGACCTGAGCTATATAAAAAGCTAGCTAAGCATCAGCCCAGGGG CAAGTCAGTAGGTGGCTTTCCTCCTTGCTTCCTGCCTTTACTCCTGCTTGAGTTTCTGCCCTGACTGCCC TCAGTGATGGACGGTGACCTGGAAGTGTAAGCCAAATAAAGCTTTTCCTCCTCAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30102203 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30102203 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11706 same embryo
	EMAGE:11703 same embryo
	EMAGE:11707 same embryo
	EMAGE:11704 same embryo
	EMAGE:11708 same embryo
	EurExpress:euxassay_006868 same experiment
	MGI:4826439 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source