EMAGE mouse gene expression spatial database

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EMAGE:11716

Manba mannosidase, beta A, lysosomal ( MGI:88175)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007081. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007081_01	euxassay_007081_02	euxassay_007081_03	euxassay_007081_04

euxassay_007081_05	euxassay_007081_06	euxassay_007081_07	euxassay_007081_08	euxassay_007081_09

euxassay_007081_10	euxassay_007081_11	euxassay_007081_12	euxassay_007081_13	euxassay_007081_14

euxassay_007081_15	euxassay_007081_16	euxassay_007081_17	euxassay_007081_18	euxassay_007081_19

euxassay_007081_20	euxassay_007081_21	euxassay_007081_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11716_wholemount_strong.wlz

11716_wholemount_moderate.wlz

11716_wholemount_weak.wlz

11716_wholemount_possible.wlz

11716_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11716_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4497
Entity Detected:	Manba, mannosidase, beta A, lysosomal ( MGI:88175)
Sequence:	sense strand is shown >T4497 GCGAGCGGTCAGCATGCATCTTCACCTGCTCTTGATATTGGCGCTATTTCGGGCCGGCTGCGTGGTGGCA GGTCCTAGCTACAGCCTGAGCGGGAGCTGGCGGGTGAGCAACGGGAATGGCTCCCTGGAGCTACCGGCGA CTGTCCCCGGCTATGTGCACAGCGCCCTGCACCAGCACGGGCTGATCCAGGATCCTTACTACAGATTTAA TGACCTGAACTATAGATGGATTTCCTTAGATAACTGGACCTACAGCACGGAATTTAAAATCCCCTTTAAT CTCAGTGAATGGCAGAAGGTAAAGTTGATCTTTGATGGAGTTGACACGGTTGCAGAAATCCTGTTCAACA ATGTCACCATTGGGAAGACAGACAACATGTTCACTGGATACAGCTTTGACATCACCAACGTAGTCAAAGA CGTGAACTCCTTAAAGCTGCAGTTCCGGTCAGCAGTGCAGTATGCAGAGTGTCAGAGCAAGGCTCACACT AGCTACCGGGTGCCCCCAGAGTGCCCCCCGGTGGAGCAGAAGGGTGAATGTCACGTCAACTTCATCCGAA AGGCACAGTGTTCCTTCAGCTGGGACTGGGGCCCTTCCTTCCCCTCTCAGGGAATCTGGAAAGATGTTAG AATCGAAGCCTATAACATTGCTCATCTGGATTACCTCACGTTTTTGCCAGTATATGATAACGCTTCTCAG GCCTGGAATATTGAAATAAAGGCTTCTTTTGATGTCGCCAGCTCGAAGTCCGTTGGTGGTCAGGTGACAG TAGCCATCCCTCAACTGAAAACACAGCAGACAAACGACATTGAACTTCAACAAGAGCAAAGGATTGTTAA ACTACTGGTCAAAATTAGGAAGGACGTTGCTGTGGAGACCTGGTGGCCTCGTGGACATGGGAACCAGACT GGGTACAACATGACAATTCTCTTTGCTCTGGATGGAGGCTTAAAAATTGAGAAAGCAGCCAAGGTTTATT TCAGGACAGTGCAACTGATAGAAGAGGGCATAAAAGGGTCTCCTGGTCTGAGTTTCTACTTCAAAATTAA TGGGCTTCCTATATTTCTGAAAGGCTCAAATTGGATTCCAGCCGACTCCTTCCAAGACAAAGTCACTTCC GATCGGCTGCAGCTCCTTTTCCAGTCCGTCGTGGATGCGAACATGAACACTCTGCGCGTGTGGGGGGGTG GCATCTACGAGCAGGATGAGTTCTATGCGCTCTGTGATGAACTTGGGATAATGGTGTGGCAGGACTTTAT GTTTGCCAGTGCCCTTTATCCAACTGAGCCTGGCTTCTTAGCCTCCGTGAGGAAAGAAGTCACCTACCAG GTCAGGAGACTGAAATCTCACCCCTCCATCATCATATGGAGTGGTAATAATGAGAATGAAGTGGCTCTCA GCGTGAACTGGTTCCACGTAAATCCCAGGGACATGAAAACGTACATTGACGACTACGTGACCCTCTATGT GAAGAACATCAGGAAGATTGTCTTATCAGAAGACAAGAGCCGTCCTTTCATTGCATCCAGCCCAACCAAC GGGATGAAAACCATGGAGGAAGGCTGGATCTCTTACGACCCTTATAGCATCCAGTATGGTGATATTCATT TTTATAACTATGCCGATGACTGCTGGAATTGGAAGATCTTCCCAAAAGCTCGATTAGTGTCTGAATATGG CTACCAGTCCTGGCCTTCTTTTAGCACACTAGAAAAGGTCTCATCTCAGGAGGACTGGGCTTACAACAGC CGCTTCTCCCTTCATCGGCAGCATCATGAAGATGGCAACCATCAGATGCTGCACCAGGTCAAGATGCATT TCAAACTCCCCCAGGGGACAGACCCATTGCGCACATTTAAAGACACTATCTACCTTACTCAGGTGATGCA AGCCCAGTGCATCAAAACAGAAACCGAGTTCTACCTGCGCAGCCGCAGCGAGATAGTGGACGGAAAAGGC CACACCATGGGGGCACTGTACTGGCAGCTGAACGATATCTGGCAGGCTCCTTCCTGGGCCTCTCTTGAGT ACGGAGGGAAATGGAAAATGCTGCATTACTTCGCTCGGCGTTTCTTCGCTCCGCTGTTGCCAGTGGGATT TGAAGATGAAGGTGTTTTTTACGTCTATGGCGTCTCAGATCTTCACAAAGACCACCACACGCAGCTCACC GTGAGGCTCCATCACTGGAGCTCCCCGAAGCCTTTGTGCTCCCTCGTGAATTCAAGTATTGTGGTTAAGG CCGGAGAGGCAGTGGTTCTGTTCCAGATGCCGGTGTCTGAGCTGCTGAAGAGATGCAGGGGATGCACGAG GGAGACATGCGTGGTCTCCTTTTACTTTTCAACTGACAAAGAACTCTTCAGCCCAACCAACTACCACTTC CTGTCCTCCCTGAAGGACGCTAAAGGGCTGCTCGAGGCCAACATCACTGTCAACATCTCTCAGAAGGGCA ATGTTTTTGTTTTTGATCTGGAAACGTCTGCGGTCGCTCCCTTTGTTTGGTTGGATGTAGGAAGTATCCC CGGGAGATTTAGTGATAACGGCTTCCTCATGATTCGGAAGAAACTCTCGGTACTGTTTTACCCTTGGAAG CCCACCAGCAAGAGTGAGCTGCAGCAGGCCTTCAGCGTCACCTCCCTGACGGATACCTACTGAAGGGACA TGGCTTGTAATTCAGTGGACACTGGGGACAAAGGCTTTCTAAAGCATGGCTGGAGAAGAAGGCAGCCTGA GATAAAACCGTCAAGAAGTATCTGCTGCCTGGGGTGCTATGACTTCCCAGTGAATACTGTTCATCCTGGT GATGTCTTTAGACAAGGTCATGCCCGGGGATTCTTGAATCTCCCAGGATTGTGGTTTTGTCCCACAGCTA AAGCACGGCCAGAAGACTCATACCACCCAATGACCTTTTTGGGAGACCACTAGACTGTGACATGATTGTG TGGAACCAAATGTGGAGGTTCTGAAGTAATGTAGGCCTTTCATAGCACCAGGAATTCTACCCACTGTAGG TGAAGTGTGTGGCTAGGATTGCCAGAGGAAATGGGAACTCAGATCCTACAATAGAGATTGCTTGATGGCA GCCATATTGATTGTTGGTAGACAGCTGAGGGGCCTGAATGTTGTATCTGATTACTATCTTTAGGTAGGAT TCCCTTTTTCATATAGTTTGGCAATAACTCTCTTTCTGAAAAGACCAAGAGATCTTTGCAGCCTAGGCTG ATTGTGCTGTCACCTGTGTAGTCTCAGACTCTGGAGGCAGAGGCAGGAGGACCACAAATTCAAAGTTGGC CCCGACTATACTGTTGAATTTTTCACTTCTCCTCGTCCTGAGTGTTCTTGTGAGCTTTAGGCTTACCACT GATTCTGATGGACTCTCGAATCCAAAGTTAGGAGCTACATTTAAACTCATTGGCTCTGCTGTGGAAGTGG CAAAAAATGTTAATAAGTAGCTATTTTGGGCACTGGTTTGTGGGCTTTTTGGTATGACTTTTATACTTTT TCTATATACTATATATATATATAAATAAAAACCCACAGATTATATAAATTGCACTGACAACTTGAAAGTT TATTTGCGCTCTGTTTAACCATGTAATAAACATTTCATTGCATACAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4952710 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4952710 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11720 same embryo
	EMAGE:11717 same embryo
	EMAGE:11719 same embryo
	EMAGE:11718 same embryo
	EMAGE:11721 same embryo
	EurExpress:euxassay_007081 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source