EMAGE mouse gene expression spatial database

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EMAGE:11728

Loxl4 lysyl oxidase-like 4 ( MGI:1914823)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006688. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006688_01	euxassay_006688_02	euxassay_006688_03	euxassay_006688_04

euxassay_006688_05	euxassay_006688_06	euxassay_006688_07	euxassay_006688_08	euxassay_006688_09

euxassay_006688_10	euxassay_006688_11	euxassay_006688_12	euxassay_006688_13	euxassay_006688_14

euxassay_006688_15	euxassay_006688_16	euxassay_006688_17	euxassay_006688_18	euxassay_006688_19

euxassay_006688_20	euxassay_006688_21	euxassay_006688_22	euxassay_006688_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11728_wholemount_strong.wlz

11728_wholemount_moderate.wlz

11728_wholemount_weak.wlz

11728_wholemount_possible.wlz

11728_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11728_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

strong

regional

strong expression: see section 17 18 19 20 moderate expression: see section 01 02 03 04 05 21 22 23 weak expression: see section 06 07 08 09 15 16

humerus

moderate

regional

moderate expression: see section 01 02 23

otic capsule

weak

regional

weak expression: see section 08 09 15 16

viscerocranium

weak

regional

Expression in the turbinate bone.

mandible

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 16 17 18 19 20 21 22

maxilla

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 17 18 19 20 21 22

axial skeleton

moderate

regional

moderate expression: see section 11 12 13 14 15 16

orbito-sphenoid

strong

regional

strong expression: see section 01 02 03 04 05 06 22 23

clavicle

strong

regional

strong expression: see section 08 09 10 16 17 18

scapula

moderate

regional

moderate expression: see section 02

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8109
Entity Detected:	Loxl4, lysyl oxidase-like 4 ( MGI:1914823)
Sequence:	sense strand is shown >T8109 CCGGCCCAGCCCCGACCTCCACATCCTGCCGGCGCTCTGAAATCACCATGATGTGGCCCCAACCACCCAC CTTCTCCCTGTTCCTGCTACTGCTGCTAAGCCAAGCCCCTTCCAGTAGGCCACAGTCATCAGGCACCAAG AAGCTCAGGCTTGTGGGGCCAGCGGACAGACCAGAGGAGGGCCGCTTGGAGGTGCTGCACCAGGGCCAGT GGGGCACGGTGTGTGATGATGATTTCGCTCTCCAGGAGGCTACTGTGGCCTGCCGACAGCTGGGCTTTGA GTCAGCCTTGACCTGGGCACACAGTGCCAAGTATGGTCAAGGAGAGGGTCCCATCTGGCTGGACAATGTT CGTTGTTTGGGCACCGAGAAGACCCTAGATCAGTGTGGCTCTAACGGCTGGGGTATCAGTGACTGCAGAC ACTCAGAAGATGTTGGGGTGGTATGTCACCCACGGCGCCAGCACGGATATCACTCTGAGAAGGTCTCCAA TGCCCTCGGGCCTCAGGGCCGGCGGCTAGAAGAGGTACGGCTGAAACCCATCCTCGCCAGTGCCAAAAGG CACAGCCCAGTGACTGAAGGGGCTGTGGAAGTACGGTACGACGGCCACTGGAGGCAGGTGTGTGACCAGG GCTGGACCATGAACAACAGCAGGGTTGTATGCGGGATGCTGGGCTTTCCCAGTCAGACATCTGTCAACAG CCACTACTACAGAAAAGTCTGGAATCTGAAGATGAAGGATCCCAAGTCTAGGCTCAACAGCCTGACAAAA AAGAATTCCTTCTGGATTCACCGGGTTGACTGTTTCGGGACAGAGCCCCACTTGGCCAAGTGCCAGGTAC AGGTGGCTCCAGGAAGGGGCAGGCTTCGGCCAGCCTGTCCAGGCGGCATGCACGCTGTGGTCAGCTGTGT GGCAGGGCCCCACTTCCGCCGACAGAAGCCAAAGCCCACGCGCAAGGAGTCCCATGCAGAGGAGCTGAAA GTGCGCCTGCGCTCTGGGGCTCAGGTGGGTGAGGGCCGTGTGGAAGTGCTCATGAACCGCCAGTGGGGCA CAGTCTGTGACCACAGGTGGAACCTCATCTCAGCCAGCGTCGTGTGTCGCCAGCTTGGCTTTGGCTCTGC CCGGGAGGCCCTTTTTGGGGCCCAGTTGGGTCAAGGGCTAGGACCCATCCACCTGAGTGAGGTGCGCTGC CGGGGGTATGAGCGGACCCTGGGTGACTGCCTTGCCCTGGAAGGGTCCCAGAATGGTTGTCAACATGCAA ACGACGCTGCTGTCAGGTGCAACATCCCAGACATGGGCTTCCAGAACAAGGTGCGCTTGGCTGGTGGGCG CAACTCCGAAGAGGGAGTGGTGGAGGTGCAGGTGGAGGTGAATGGGGTCCCACGATGGGGGACTGTATGC AGTGACCACTGGGGGCTCACCGAAGCCATGGTGACCTGTCGGCAACTTGGTCTGGGATTTGCCAACTTTG CTCTCAAGGACACCTGGTACTGGCAGGGGACACCAGAGGCCAAAGAAGTGGTGATGAGTGGAGTTCGCTG CTCCGGCACAGAAATGGCCCTGCAGCAGTGTCAGAGGCATGGGCCGGTGCACTGTTCCCACGGCCCAGGG CGCTTCTCGGCTGGCGTTGCTTGTATGAACAGTGCTCCAGACCTCGTGATGAACGCCCAGCTGGTACAAG AGACGGCGTACTTGGAGGATCGTCCACTCAGCATGCTGTACTGTGCTCACGAGGAAAACTGCCTCTCCAA GTCCGCTGATCACATGGACTGGTCCTACGGGTACCGGCGCTTGCTGCGCTTCTCCTCACAGATCTACAAC CTTGGCCGGGCCGACTTCCGTCCCAAGGCTGGACGCCACAGCTGGATTTGGCACCAGTGCCACAGGCACT ACCACAGCATCGAAGTCTTCACTCATTATGACCTGCTCACGCTCAATGGCTCCAAGGTGGCCGAGGGACA CAAGGCCAGCTTCTGTCTAGAAGATACAAACTGCCCCTCAGGAGTGCAGCGGCGCTATGCATGTGCCAAC TTTGGGGAACAGGGAGTGGCTGTAGGCTGCTGGGACACCTACCGGCATGACATCGATTGCCAGTGGGTGG ATATCACAGATGTGGGTCCAGGGGACTATATCTTCCAGGTGGTTGTGAACCCCACAAACGATGTTGCAGA GTCCGATTTCTCCAATAACATGATACGGTGCCGCTGCAAGTATGATGGACAGCGAGTCTGGTTGCACAAC TGCCACACAGGAGATTCCTACCGAGCCAATGCAGAGCTCTCCCTGGAGCAGGAACAGCGACTCAGGAACA ACTTGATCTGAAGCCATTACTGCACTCCCAGCTCTGCTCACACACCAGATACCTCAGCTGACTGGAGCCA TGCCCTTCACAAAGCCCTGACTCACAGGACAAGGGGCTAGTGACAAGGAGCACCAAGAAGCTGCTCAGGA GGCCTTTCAGTGGCCAGATCATCACCCGGGATGGCAGTTCTCTCAGGATGGCTCTGGGCCAGCTCAACCT TCTCTTCCTTCAGGAGACTCGATCTTCTTTACAACTTGATGCACAGTTCCCAGTTTCAGGAGCTCTAAGT TCCTCAGGGATGAACTGTGACCAAGGCCCCCTCTAAGTGGTGCTTTGCAAATGTCTTGGAGGACCAAAGG ACAGACGACCCGAGAACACAAGCTGTGGATGGAGTCGTTTTCTCTGCTATACGCTCCACGCAAAAGGACC ATGTCAAAGTCACACCTGGCAGAGACGCTGGTGAACACAGTCCCTCAGCTTACCCTCACTTAGAACTCGC ATCTCAGGCTCTGAAGCCTCTCCTTGCATCTTTACCTTCATTCGGTCACACATGGTGTTTCCAATATCCC TGAACCCTCAGGCCTCCTCCATTTCCTGATGGGTCAACACCTCGATTATTGGGGCTGGGGAGCAAGTTTC ACAGAATGACGAGACAGGGCCTTTCCTGCAGAGTAGTCAGAAAGCAAGACGAAGGCTGAGGTCACATGAA TTCAGCCTCGGAGGACCTCTGCCCAGGGAGGTGCCATATCTGGCAGGCAGGCCAGTCTCTTAGAATCACC CACATTGTAGGGTTAGCCTAAATTTCAGATTTAACCAAAGCCACTTCACCTTAAACTTTTGCAATTGAGA AGAAATTGGTCAGCCAAGCCCCTCTGCAGGAACACCAAGACAGGTCCAGTAGAGTTAGGAACAGAAGACG GAGTTAACAAGAAGTGAGAAGGAAACCTGGGAGAGGCCACCATCCAGTGAGGCGGCTGTGCTCTGTTCTT GCTCAGGTGGTATAAGACCTGGAGTCTTCCAGGGCATGCCTGGACCTTTTCTCCACTGACATACACACAG ATGATCCTTCTAGGTCTTATAATGCCAACAAAGGTTGCTCACTATGGGCTGCCACAAAGGCCAGACCAGC TGAGCTTCTGCAGCTTCCACTGCACTACACTCTGCCCTCTTCCCTCCAGAGCTGGATGACGCTCGGAACA CAATCCTTGGCACAGCCCACCCTAGTACATTTCTTGGGTCTCACACTAACCTGCTTCACCGTTACGCTGC CCAAGGTCAACAGTGAATCTTGGGTCAGGAAGGCTGAGAGGTGAAGGGGAGGAATGAGAAGGTGTGACCC AGACTTTAGACTTTTATAACAGAGGCTACAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30059745 using vector (pSPORT1) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30059745 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11725 same embryo
	EMAGE:11726 same embryo
	EMAGE:11727 same embryo
	EMAGE:11724 same embryo
	EMAGE:11722 same embryo
	EMAGE:11723 same embryo
	EurExpress:euxassay_006688 same experiment
	MGI:4825964 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source