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EMAGE:11741

Pacsin1 protein kinase C and casein kinase substrate in neurons 1 ( MGI:1345181)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:11741 EMAGE:11741 EMAGE:11741 EMAGE:11741 EMAGE:11741
"Pseudo-wholemount" of euxassay_007123. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_007123_01 euxassay_007123_02 euxassay_007123_03 euxassay_007123_04
EMAGE:11741 EMAGE:11741 EMAGE:11741 EMAGE:11741 EMAGE:11741
euxassay_007123_05 euxassay_007123_06 euxassay_007123_07 euxassay_007123_08 euxassay_007123_09
EMAGE:11741 EMAGE:11741 EMAGE:11741 EMAGE:11741 EMAGE:11741
euxassay_007123_10 euxassay_007123_11 euxassay_007123_12 euxassay_007123_13 euxassay_007123_14
EMAGE:11741 EMAGE:11741 EMAGE:11741 EMAGE:11741 EMAGE:11741
euxassay_007123_15 euxassay_007123_16 euxassay_007123_17 euxassay_007123_18 euxassay_007123_19
EMAGE:11741 EMAGE:11741 EMAGE:11741 EMAGE:11741 EMAGE:11741
euxassay_007123_20 euxassay_007123_21 euxassay_007123_22 euxassay_007123_23 euxassay_007123_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:11741Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
11741_wholemount_strong.wlz
11741_wholemount_moderate.wlz
11741_wholemount_weak.wlz
11741_wholemount_possible.wlz
11741_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:11741_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
brain
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
adenohypophysis
moderate moderate
regionalmoderate expression: see section 11 12 13 14 15 16 17
facial vii ganglion
moderate moderate
regionalmoderate expression: see section 05 06 22 23
glossopharyngeal ix ganglion
moderate moderate
regionalmoderate expression: see section 07 19
trigeminal v ganglion
moderate moderate
regionalmoderate expression: see section 04 05 06 07 08 09 10 19 20 21 22 23 24
vagus x ganglion
moderate moderate
regionalmoderate expression: see section 08 18
vestibulocochlear viii ganglion
moderate moderate
regionalmoderate expression: see section 07 08 18 19 20
spinal cord
moderate moderate
regionalmoderate expression: see section 08 09 10 11 12 13 14 15 16 17 18
dorsal root ganglion
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 11 12 13 16 17
nasal cavity olfactory epithelium
moderate moderate
regionalmoderate expression: see section 11 12 13 14 15 16 17 18 19 20 21 22 23
nasal cavity respiratory epithelium
moderate moderate
regionalmoderate expression: see section 16 19 20
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T4359
Entity Detected:Pacsin1, protein kinase C and casein kinase substrate in neurons 1 ( MGI:1345181)
Sequence:sense strand is shown

>T4359
CTCCTCCTCCTTTTCCTCCTCCTCCCCCGGGAAGCCGGCCGGGCTTGAGGCTGGGCCGCCGCCGCCGCCC
GCTTTGCCACCCGCCCCGCTGATGGTGTCCGGTGCTCCGGCGCCCAGCCTGTGAGCAGCAGGCCTGCTAA
CTGCAGATCCACAACCGCACAGCTCGCTACAGGTGCACCATGTCTGGCTCCTACGATGAGGCCTCAGAGG
AGATCACAGATAGCTTCTGGGAGGTGGGGAACTACAAGCGGACGGTGAAGCGCATCGACGATGGGCACCG
CCTGTGCAACGACCTCATGAGCTGCGTGCAGGAGCGCGCCAAGATCGAGAAGGCATACGCGCAGCAGCTC
ACCGACTGGGCCAAGCGCTGGCGCCAGCTCATCGAGAAAGGTCCTCAGTATGGCAGCCTGGAGCGGGCGT
GGGGCGCCATGATGACAGAAGCAGATAAGGTCAGCGAGCTGCACCAGGAGGTGAAGAACAGCCTGCTGAA
TGAGGACCTGGAGAAAGTCAAGAACTGGCAGAAGGATGCCTATCACAAGCAGATCATGGGTGGCTTCAAG
GAGACGAAAGAGGCCGAGGATGGCTTCCGAAAGGCCCAGAAGCCCTGGGCTAAAAAGATGAAGGAGCTAG
AGGCGGCCAAGAAGGCCTATCACTTGGCTTGTAAGGAGGAAAGGCTGGCCATGACCCGGGAGATGAACAG
TAAGACAGAGCAGTCGGTCACCCCTGAACAGCAGAAGAAACTTGTGGACAAAGTGGACAAATGCAGACAG
GATGTGCAAAAGACTCAGGAGAAGTATGAGAAGGTGCTGGAAGATGTGGGCAAGACCACACCACAGTACA
TGGAGGGCATGGAGCAGGTGTTTGAGCAGTGCCAGCAGTTTGAGGAGAAGCGGCTGGTCTTCCTGAAGGA
AGTCCTGCTGGATATCAAACGGCATCTCAACCTAGCGGAGAACAGCAGCTACATGCATGTCTACCGAGAA
CTGGAGCAGGCCATCCGGGGGGCCGATGCCCAGGAGGACCTCAGGTGGTTCCGCAGCACCAGTGGCCCCG
GGATGCCCATGAACTGGCCGCAGTTCGAGGAGTGGAACCCAGACCTCCCGCACACCACTGCCAAGAAGGA
GAAACAGCCTAAGAAGGCAGAGGGGGCCACCCTGAGCAATGCCACTGGGGCTGTAGAATCCACATCCCAG
GCTGGGGACCGTGGCAGTGTTAGCAGCTATGACCGAGGCCAAACATATGCCACCGAGTGGTCAGACGATG
AGAGCGGAAACCCCTTCGGGGGCAATGAGGCCAATGGTGGCGCCAACCCCTTCGAGGATGATGCCAAGGG
AGTTCGTGTACGGGCACTCTATGACTACGACGGTCAGGAGCAGGATGAGCTCAGCTTCAAGGCCGGAGAT
GAGCTCACCAAGCTCGGAGAGGAAGACGAACAGGGTTGGTGCCGCGGGCGGCTGGACAGCGGACAGCTGG
GCCTCTATCCTGCCAACTACGTTGAGGCTATATAGCTACCTTGCCCACCCGACTCCTCTCAGTCCTTGTC
CACCGCCTTCCACCCTTCCCCTCCCCCTTGCCATAGAGTTCCAGACATATTTTCCGATCAAGCTTTTATT
TTTTTAAAAGTCAAAACAGAACAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:4527708 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4527708 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:11743 same embryo
 EMAGE:11740 same embryo
 EMAGE:11742 same embryo
 EMAGE:11738 same embryo
 EMAGE:11739 same embryo
 EurExpress:euxassay_007123 same experiment
 MGI:4827023 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS