EMAGE mouse gene expression spatial database

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EMAGE:11764

Ccl7 chemokine (C-C motif) ligand 7 ( MGI:99512)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006936. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006936_01	euxassay_006936_02	euxassay_006936_03	euxassay_006936_04

euxassay_006936_05	euxassay_006936_06	euxassay_006936_07	euxassay_006936_08	euxassay_006936_09

euxassay_006936_10	euxassay_006936_11	euxassay_006936_12	euxassay_006936_13	euxassay_006936_14

euxassay_006936_15	euxassay_006936_16	euxassay_006936_17	euxassay_006936_18	euxassay_006936_19

euxassay_006936_20	euxassay_006936_21	euxassay_006936_22	euxassay_006936_23	euxassay_006936_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11764_wholemount_strong.wlz

11764_wholemount_moderate.wlz

11764_wholemount_weak.wlz

11764_wholemount_possible.wlz

11764_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11764_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

upper leg muscle

weak

spotted

weak expression: see section 02 03 04 05 06 07 08 23 24

diaphragm

weak

spotted

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 19 20 21 22 23

lower leg rest of mesenchyme

weak

spotted

weak expression: see section 02 03 04 05

vertebral axis musculature

weak

spotted

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

pituitary gland

weak

spotted

weak expression: see section 11 12 13 14 15

heart ventricle

weak

spotted

weak expression: see section 06 07 08 09 10 11 12 13 14 15 16

left lung

moderate

regional

moderate expression: see section 02 03 04 05 06 07 08 09 10 11 12

right lung

moderate

regional

moderate expression: see section 13 14 15 16 17 18 19 20 21 22 23 24

tail paraxial mesenchyme

weak

regional

weak expression: see section 13 14 15 16 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9964
Entity Detected:	Ccl7, chemokine (C-C motif) ligand 7 ( MGI:99512)
Sequence:	sense strand is shown >T9964 AGAGAAGCAAGGCCAGCACAGAGTCTGCCAGCTCTCACTGAAGCCAGCTCTCTCACTCTCTTTCTCCACC ATGAGGATCTCTGCCACGCTTCTGTGCCTGCTGCTCATAGCCGCTGCTTTCAGCATCCAAGTGTGGGCCC AACCAGATGGGCCCAATGCATCCACATGCTGCTATGTCAAGAAACAAAAGATCCCCAAGAGGAATCTCAA GAGCTACAGAAGGATCACCAGTAGTCGGTGTCCCTGGGAAGCTGTTATCTTCAAGACAAAGAAGGGCATG GAAGTCTGTGCTGAAGCCCATCAGAAGTGGGTCGAGGAGGCTATAGCATACTTAGACATGAAAACCCCAA CTCCAAAGCCTTGAAGAAATGTGCCTGAACAGAAACCAACCTAGGAGCCAAGAAGCAAAAATTCCTCACC GCTGTTCTTTCTGAGAACTGTTGATGAAATGTGTTGATCACGGTCCTAAGGGATAGGAGCTGTCTGTAGG AATGTGAAACAGTCACGCCTAAGGAATGGTCTTTAAGTTATTAATATTTTTATTTAATTAGCCATGTACT TTGGTGTGATTTGAATGTAAAGCTCTGGAGACCTCATGTCACTTTAACATTGTGTTAGCTGCAGAATTCT CCCCCTTTCCCCATTTTTACTTTGTTCTTGTATTATGAAGGGACTTTGCAAGAATCAGTGCAAAGATTTT ATTTAAAAACTTTTAGGATAAAATCATAATGTTATGATACTGTGTGGTTTTTAAATGTATGACTACTGAA GTTTCATATAAAATGTATTTTTGCACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA A
Notes:	The probe template was PCR amplified from IMAGE:30276350 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30276350 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11762 same embryo
	EMAGE:11766 same embryo
	EMAGE:11761 same embryo
	EMAGE:11763 same embryo
	EMAGE:11765 same embryo
	EurExpress:euxassay_006936 same experiment
	MGI:4823703 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source