EMAGE mouse gene expression spatial database

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EMAGE:11789

Skiv2l superkiller viralicidic activity 2-like (S. cerevisiae) ( MGI:1099835)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007039. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007039_01	euxassay_007039_02	euxassay_007039_03	euxassay_007039_04

euxassay_007039_05	euxassay_007039_06	euxassay_007039_07	euxassay_007039_08	euxassay_007039_09

euxassay_007039_10	euxassay_007039_11	euxassay_007039_12	euxassay_007039_13	euxassay_007039_14

euxassay_007039_15	euxassay_007039_16	euxassay_007039_17	euxassay_007039_18	euxassay_007039_19

euxassay_007039_20	euxassay_007039_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11789_wholemount_strong.wlz

11789_wholemount_moderate.wlz

11789_wholemount_weak.wlz

11789_wholemount_possible.wlz

11789_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11789_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8404
Entity Detected:	Skiv2l, superkiller viralicidic activity 2-like (S. cerevisiae) ( MGI:1099835)
Sequence:	sense strand is shown >T8404 GGGAGCTGCCGCGGCTCCAGGATGATGGAGACGGAGCGACTCGTTCTGCCTCCCCTAGATCCCCTGAACT TACCCCTTCGAGCTCTGGAAGTGGGGTGCACTGGCCGTTGGGAGCTGCTGAACGTGCCAGGGCCTCCAGA GAGCACTCTCCCCCATGGCCTCCCTCCATGCGCCCCAGATCTGTGCCAGGAAGCAGAGCAGCTGTTTCTG TCGTCTCCAGCCTGGCTTCCTCTACACGGTGTAGAGCACTCAGCTCGAAAATGGCAGAGGAAGACGGATC CCTGGTCACTCCTGGCTGCCGTGGAGACTCCAGTCCCATCTGACCTTCAGGCCCAGAGACACCCAACCAC AGGCCATATACTGGGCTATAAGGAGGTCCTGCTGGAGAACACAAATCTGTCAGCTACAACCTCCTTGTCC CTTCGCCGGCCTCCAGGGCCAGCCTCCCAGTCGCTATGGGGCAATCCAACACAGTACCCTTTCTGGCCAG GTGGGATGGATGAGCCCAGCATAACTGATCTGCATACTCGGGAGGAAGCTGAGGAGGAGATAGACTTTGA GAAAGATCTTCTCACTGTCCCACCTGGCTTCAAGAAAGGGGTGGATTTTGCCCCCAAAGATCATCCAGCT CCTGTGCCAGGTTTGCTCAGCCTCAGCCGTCTGCTGGAGCCTCTTGATCTGAGTGGAGGTGACGAGGATG AAGGCGAGGCAGCAGGAGGCCCTAGAGGGGACAATGCCTCCCCCTCCCCCTCTGGTACTCCCCTGGTCCG AGCAAGCAGCTTGGAGGATCTAGTGTTGAAGGAAGCTGCCACAGTCGTATCTACCCCGGAGCCTCCCAAA CCTCCACCTCAGGAACAGTGGGCTGTGCCTGTGGACGTCACCTCCCCTGTGGGCGACTTTTACCGTCTCA TCCCTCAGCCAGCTTTCCAGTGGGCATTTGAGCCAGATGTGTTTCAGAAGCAGGCCATCCTGCACTTGGA GCAGCACGACTCTGTCTTTGTTGCAGCTCACACATCTGCTGGGAAGACAGTGGTGGCTGAATATGCCATC GCCCTGGCCCAGAAACACATGACTCGTACCATCTACACTTCTCCTATCAAAGCCCTGAGCAACCAGAAGT TTCGGGACTTCCGGAACACATTTGGGGATGTAGGGCTGCTCACAGGGGATGTGCAGCTGCACCCAGAGGC CTCCTGCCTCATCATGACCACGGAGATCCTTCGCTCCATGCTGTACAGTGGCTCCGACGTCATCCGAGAC CTGGAGTGGGTCATCTTTGATGAAGTCCACTATATCAATGATGCTGAGCGTGGGGTCGTGTGGGAGGAGG TGCTCATCATGCTTCCTGAGCACGTCTCCATCATCCTTCTGAGTGCCACCGTCCCCAATGCCCTGGAGTT TGCTGACTGGATCGGGCGGCTGAAGCGGAGGCAGATCTATGTGATCAGCACGGTTGCCCGGCCGGTCCCC CTGGAGCACTATCTCTTCACAGGGAACAGCCCCAAGACGCAGGGCGAGCTCTTCCTGCTGCTGGACTCCC GAGGGGCCTTCCACACCCAGGGGTACTATGCTGCAGTGGAGGCCAAGAAAGAGAGGATGAGCAAGCACGC CCAGACTTTCGGGGCCAAGCAGCCCACGCACCAGGGTGGGCCTGCACAGGACCGCGGGGTGTACCTGTCC CTGCTGGCTTCCCTCCGGACCCGGGCTCAGTTGCCAGTGGTAGTGTTCACCTTCTCCCGAGGCCGCTGTG ATGAGCAGGCTTCGGGCCTCACCTCTCTAGACCTCACGACAAGCTCGGAGAAGAGTGAGATCCACCTCTT CCTGCAGCGCTGCCTTGCACGGCTCCGAGGCTCTGACCGACAGCTACCCCAGGTCCTGCACATGTCGGAG CTCCTTCGCCGAGGCCTAGGTGTGCACCACAGCGGCATCCTGCCCATCCTTAAGGAGATTGTGGAAATGC TTTTCAGCAGGGGCCTGGTCAAGGTCTTGTTTGCCACCGAGACTTTTGCCATGGGGGTAAACATGCCAGC CAGGACGGTGGTGTTTGACTCCATGCGCAAACATGATGGCTCTACCTTCCGGGACCTGCTGCCTGGGGAG TACGTGCAGATGGCAGGCCGGGCAGGCCGGAGGGGCCTGGACCCCACGGGCACCGTCATCCTTCTCTGTA AGGGCCGAGTGCCTGAGATGGCAGATCTACACCGCATGATGATGGGGAAACCATCCCAACTTCAGTCCCA GTTCCGCCTCACATATACCATGATCCTCAACCTGCTGCGGGTGGATGCCCTCAGAGTGGAGGACATGATG AAGAGGAGCTTCTCAGAGTTCCCATCCCGCAAGGACAGCAAGGCCCATGAGCAGGCTCTGGCTGACCTCA CCAAGAGGCTGGGGGCTTTGGAGGAGCCTGATGTGACTGGTCAGTTGGCTGACCTACCTGAGTATTACAG CTGGGCGGAGGAGCTGACAGAGACCCAGAACATGATCCAGCGACGCATCATGGAGTCTGTGAATGGGCTG AAATCTCTCTCAGTGGGAAGGGTGGTAGTTGTGAAGAATGAGGAACATCACAATGCACTGGGTGTGATCC TGCAGGTCTCCTCAAACTCCACCAGCAGGGTATTTACAACATTGGTCTTGTGTGATAAGCCTGTTGTGTC TGATAACCCAAGGGACAAGGGGCCAGCCACTCCAGATGTGCCCCACCCAGATGACCTTATAGGCTTCAAG CTGTTCCTGCCTGAAGGGCCCTGTGAGCACACCGTGGCCAAGCTCCAGCCAGGAGATGTGGCTGCCATCT CTACCAAAGTGCTCCGGGTGAATGGGGAGAAGATCTCTGAGGACTTTAGCAAGAGGCAGCAACCAAAATT CAGAAAGGACCCTCCCCTTGCGGCTGTGACCACTGCTGTTCAGGAGCTGCTGCGTCTGGCTCAGTCCTAT CCAGCAGGACCCCCAACCCTTGACCCTATCAACGACCTGCAACTCAAGGATGTGGCAGTGGTAGAAGGTG GGCTCCGGGCCCGGAAGCTGGAGGAGCTGATCCGCGGGGCTCAGTGTGTGCACAGCCCCCGGTTTCCTGC CCAGTACGTGAAGCTGCGGGAGCGAATGCAGATTCAGAAGGAAATGGAGAGGCTGCGCTTCCTCCTGTCT GACCAGTCGCTGTTACTGCTCCCTGAGTACCACCAGCGTGTAGAGGTGCTCCGGACTCTGGGCTATGTGG ATGAGGCTGGCACAGTGAAGCTGGCAGGCCGAGTGGCCTGTGCCATGAGCAGCCATGAGCTGCTCCTCAC TGAACTCATGTTTGACAATGCACTGAGCGCTCTGCGACCAGAGGAAATCGCAGCTCTGCTCTCTGGCCTG GTGTGCCAGAGCCCCGGGGACCCTGGCGACCAGCTCCCAAGTACCCTCAAACAGGGGGTAGAACGAGTCA AAGCTGTGGCCAAGCGGATTGGCGAGGTTCAGGTGGCCTGTGGCCTGAACCAGACAGTGGAGGAGTTTGT GGGAGAGCTGAATTTTGGGCTGGTAGAGGTTGTGTATGAGTGGGCTAGGGGTATGCCCTTCTCCGAGTTG GCAGGGCTCTCAGGGACCCCTGAGGGCTTGGTCGTCCGCTGCATCCAGCGTCTGGCTGAGATGTGTCGCT CGCTTCGAGGAGCAGCACGTTTGGTGGGTGAGCCTGTGCTGGGTGCCAAGATGGAGACGGCAGCCACCTT GTTACGGCGGGACATCGTCTTCGCAGCCAGCCTCTACACCCAGTAAAGGATGTGTGGATAGAAATGTAAC AATAAGCTGGGTTGTAGTAGCAGCGAAGGGGCAGATAAATCTCTTTGAGTTTGATGCCAACCATGTTTTG AAAAAACTAAAAACAAACAAAAAAAACCCCCTAAAAACCCAAAAATATAAATAAAGAGCAAACCAGAAAA AAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5686586 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5686586 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11791 same embryo
	EMAGE:11790 same embryo
	EMAGE:11786 same embryo
	EMAGE:11788 same embryo
	EMAGE:11787 same embryo
	EurExpress:euxassay_007039 same experiment
	MGI:4828084 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source