EMAGE mouse gene expression spatial database

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EMAGE:11792

Nr4a1 nuclear receptor subfamily 4, group A, member 1 ( MGI:1352454)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007083. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007083_01	euxassay_007083_02	euxassay_007083_03	euxassay_007083_04

euxassay_007083_05	euxassay_007083_06	euxassay_007083_07	euxassay_007083_08	euxassay_007083_09

euxassay_007083_10	euxassay_007083_11	euxassay_007083_12	euxassay_007083_13	euxassay_007083_14

euxassay_007083_15	euxassay_007083_16	euxassay_007083_17	euxassay_007083_18	euxassay_007083_19

euxassay_007083_20	euxassay_007083_21	euxassay_007083_22	euxassay_007083_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11792_wholemount_strong.wlz

11792_wholemount_moderate.wlz

11792_wholemount_weak.wlz

11792_wholemount_possible.wlz

11792_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11792_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

adrenal gland

strong

regional

strong expression: see section 09 10 11 18 19 20 21

submandibular gland primordium

moderate

regional

moderate expression: see section 07 08 09 10 16 17 18 19

vibrissa

weak

regional

weak expression: see section 04 05 06 07 08 19 20 21 22 23

peripheral nervous system

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 14 15 17 18 19 20 21 22 23

mandible

moderate

regional

moderate expression: see section 04 05 21 22

testis

moderate

regional

moderate expression: see section 06 07 08 21 22 23

clavicle

moderate

regional

moderate expression: see section 09 10 17 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5880
Entity Detected:	Nr4a1, nuclear receptor subfamily 4, group A, member 1 ( MGI:1352454)
Sequence:	sense strand is shown >T5880 GCGAAAGTTGGGGGAGTGTGCTAGAAGGACTGCGGAGCGGAGCGCACGCGGGACCAGGCTGCGACTGGGT CGCTGGTCCCGGCCACAGGGAGTGGGAGCCGGCTGGAGATGCCCTGTATTCAAGCTCAATATGGAACACC AGCAACGAGCCCAGGACCGCGTGACCACCTGACCGGTGATCCCCTGGCCCTTGAGTTCGGCAAGCCTACC ATGGACCTGGCCAGCCCCGAGACAGCACCTGCCGCACCTGCTACACTGCCCAGCTTCAGCACCTTCATGG ACGGGTACACCGGAGAGTTTGACACCTTCCTCTACCAGCTGCCGGGGACGACCCAGCCGTGCTCCTCAGC TTGTTCCTCTGCCTCCTCCACGTCTTCTTCCTCATCCTCGGCCACCTCCCCCGCTTCGGCGTCCTTCAAG TTTGAGGACTTCCAGGTGTACGGCTGCTACCCGGGCACCCTGAGCGGCCCATTAGATGAGACCCTATCCT CCAGCGGCTCTGAGTACTATGGCAGTCCCTGCTCAGCCCCCTCGCCATCTACACCCAACTTCCAGCCGTC CCAGCTTTCTCCCTGGGACGGCTCATTTGGCCACTTCTCCCCGAGCCAGACTTATGAAGGCCTCTGGGCA TGGACAGAGCAGTTGCCTAAGGCTTCTTCAGGGCCTCCGCCACCTCCAACCTTCTTCTCCTTCAGTCCTC CCACTGGCCCCAGCCCCAGCCTGGCCCAGAGTTCTCTGAAATTGTTCCCACCACCAGCCACCCACCAGCT TGGGGAGGGGGAGAGCTATTCCATGCCAGCAGCTTTCCCCGGCTTGGCACCCACCTCTCCGAACCGTGAC ACTTCCGGCATTCTGGACGCACCCGTGACCTCCACCAAGTCCCGGAGCGGGGCTTCAGGTGGCAGCGAGG GCCGCTGTGCAGTCTGTGGTGACAATGCTTCGTGTCAGCACTATGGGGTCCGCACCTGTGAGGGCTGCAA GGGCTTCTTCAAGCGCACAGTACAGAAAAGCGCCAAGTACATCTGCCTGGCAAACAAGGATTGCCCTGTG GACAAGAGGCGGCGGAACCGCTGCCAGTTCTGCCGCTTCCAGAAGTGCCTGGCTGTGGGCATGGTGAAGG AAGTTGTACGGACAGACAGCCTAAAAGGGCGGCGGGGCCGGCTACCTTCAAAACCCAAGCAGCCTCCAGA TGCCTCCCCTACCAATCTTCTCACTTCCCTCATCCGGGCACACTTGGACTCCGGGCCTAGCACTGCCAAA TTGGACTATTCCAAGTTCCAGGAACTGGTGCTGCCCCGCTTCGGGAAGGAAGATGCCGGTGACGTGCAAC AATTTTATGACTTGCTCTCTGGTTCCCTGGACGTTATCCGAAAGTGGGCAGAAAAAATCCCTGGCTTCAT TGAGCTTTGCCCAGGAGACCAAGACCTGTTGCTAGAGTCTGCCTTCCTGGAACTCTTCATCCTCCGCCTG GCATACCGATCTAAACCCGGTGAGGGGAAGCTCATCTTCTGCTCAGGCCTGGTACTACACCAGCTGCAGT GTGCCCGTGGCTTTGGTGATTGGATTGACAACATCCTGGCCTTCTCACGGTCCCTGCACAGCTTGGGTGT TGATGTTCCCGCCTTTGCCTGCCTGTCCGCTCTGGTCCTCATCACTGATCGACACGGGCTCCAGGACCCT CGTCGGGTGGAAGAGCTGCAGAATCGCATTGCTAGCTGTCTGAAGGAGCACATGGCTACCGTGGCAGGAG ACCCACAGCCGGCCAGCTGCCTGTCACGTCTGCTGGGCAAACTGCCTGAGCTTCGGACCCTGTGCACTCA AGGCCTGCAGCGCATCTTTTGCCTCAAGTTGGAGGACTTGGTACCCCCTCCACCTATTGTGGACAAGATC TTTATGGACACATTGTCTTTCTGACCCCTGCCCTGAACATGTGTGCGCACACGTGCGTGCTCTTCTGTCA CCCATGTGCCTTTAAGCCTATAGCCCACGGACCCCCAGACCACCCTACCCCCAGCCTGGTTTTGAGCTAA GACTGACGTACCTCCTCACTCCAGAAGATGGACAGAGAACTCAAGACCTGGGGGAGGGTGTGTATTCACG GGGGTGACCCCACTATTTGTCTTATCCCTCCAGCTCAGTCCTGGCCTTCGTGTGTTTTTGTAAGATAAAC CATTTTTAACACATACCACTCTGTTGTAAATAAGCTGACGCTACTGTAAATACAGAAAGGAAGAGGTTGA GATGGGGGTTGGGAGGAAGGGGTGGGGCTCCCACCAGCTGGGCGAGCCTCCAACTCGAGATCTCTTCCGC TCTCCTTCCATGTGTACATAACTGTCACTCAAGAAGGTGATTGACAGATTCTGATTTATATTTGTGTATT TTCCTGGATTTATAGGATGTGACTTTTCTGATTAATATATTTAATATATTGAATAAAAAATAGACATGTA GTTGAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3156434 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3156434 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11793 same embryo
	EMAGE:11797 same embryo
	EMAGE:11798 same embryo
	EMAGE:11795 same embryo
	EMAGE:11796 same embryo
	EMAGE:11794 same embryo
	EurExpress:euxassay_007083 same experiment
	MGI:4826785 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source