EMAGE mouse gene expression spatial database

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EMAGE:11911

Tnfaip3 tumor necrosis factor, alpha-induced protein 3 ( MGI:1196377)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008542. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008542_01	euxassay_008542_02	euxassay_008542_03	euxassay_008542_04

euxassay_008542_05	euxassay_008542_06	euxassay_008542_07	euxassay_008542_08	euxassay_008542_09

euxassay_008542_10	euxassay_008542_11	euxassay_008542_12	euxassay_008542_13	euxassay_008542_14

euxassay_008542_15	euxassay_008542_16	euxassay_008542_17	euxassay_008542_18	euxassay_008542_19

euxassay_008542_20	euxassay_008542_21	euxassay_008542_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

11911_wholemount_strong.wlz

11911_wholemount_moderate.wlz

11911_wholemount_weak.wlz

11911_wholemount_possible.wlz

11911_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:11911_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9305
Entity Detected:	Tnfaip3, tumor necrosis factor, alpha-induced protein 3 ( MGI:1196377)
Sequence:	sense strand is shown >T9305 GTTGGCAAAGCATACAACTGAAAGGCAAAAAAGGGAACTGCCCAGTCTGTAGTCTTCGGAGCCGGAGGCG GAGCGGTGAGCCAGGCCGCCGAAAGACGTGCACACCCCCTCAGCCAGCGAGCCCGATCGGGTGTCCATGG GGCTCCGCCTCGGCCCGACCCCCACCACGACCCGGACCACGATCCCGGGCTGCGGCGCGCGCCAGTGACG GCCGGCCGGCCACTTGGGCTCTGCGAGGGCTCCGCGCGGCCCCAAGAGGCCTTGTCGAGGACCATGGCTG AACAACTTCTTCCTCAGGCTTTGTATTTGAGCAATATGCGGAAAGCTGTGAAGATACGAGAGAGAACCCC AGAAGACATTTTCAAACCTACCAATGGGATCATCTATCACTTTAAAACCATGCACCGATACACGCTGGAG ATGTTCAGAACATGCCAGTTTTGCCCACAGTTCCGAGAGATCATCCACAAAGCACTTATTGACAGAAGTG TCCAGGCTTCCCTGGAAAGCCAGAAGAAGCTCAACTGGTGTCGTGAAGTCAGGAAGCTCGTGGCTCTGAA AACCAATGGTGATGGAAACTGCCTCATGCATGCAGCTTGTCAGTACATGTGGGGTGTTCAGGATACTGAC CTGGTCCTGAGGAAGGCCCTCTGCAGCACCCTTAAGGAGACAGACACTCGGAACTTTAAATTCCGCTGGC AGCTGGAATCTCTGAAATCTCAGGAATTTGTGGAAACAGGACTTTGCTACGACACTCGGAACTGGAATGA CGAATGGGACAACTTGGTCAAAATGGCATCAGCAGACACACCTGCAGCCCGAAGTGGACTTCAGTACAAT TCCCTGGAAGAAATCCACATATTTGTCCTCAGCAACATCCTCAGAAGACCCATCATTGTCATTTCAGACA AAATGCTAAGAAGTTTGGAATCTGGTTCCAATTTTGCTCCTTTGAAAGTGGGTGGGATTTATCTGCCTCT TCACTGGCCTGCCCAGGAGTGTTACAGATATCCCATCGTCCTAGGCTATGACAGCCAGCACTTTGTACCC CTGGTGACCCTGAAGGACAGTGGACCTGAACTTCGCGCTGTTCCACTTGTTAACAGAGACCGGGGTAGGT TTGAAGACTTAAAAGTTCACTTCTTGACAGATCCTGAGAATGAGATGAAGGAAAAGCTTCTAAAGGAGTA CTTGATAGTGATGGAGATCCCTGTGCAAGGCTGGGACCACGGCACGACTCACCTGATCAACGCTGCAAAA TTGGATGAAGCTAACTTACCCAAAGAAATAAATTTGGTAGACGATTACTTTGAGCTTGTTCAGCACGAAT ACAAGAAATGGCAGGAGAACAGCGATCAGGCCAGGAGAGCGGCACATGCGCAGAACCCCTTGGAGCCTTC CACACCCCAGCTATCACTCATGGATATAAAATGTGAGACACCCAACTGTCCTTTCTTCATGTCCGTGAAC ACTCAGCCTTTATTCCACGAATGCTCAGAGAGGCGCCAAAAGAATCAGAGCAAGCTCCCAAAGCTGAACT CGAAGCTAGGCCCTGAAGGACTCCCAGGCGTGGGACTTGGCTCCTCAAACTGGAGCCCCGAGGAAACCGC TGGAGGACCTCATTCAGCCCCACCCACAGCACCCAGCCTTTTTCTCTTCAGTGAGACCACTGCAATGAAG TGCAGGAGTCCTGGGTGCCCTTTTACTTTGAATGTGCAGCATAATGGATTCTGTGAGCGTTGCCACGCCC GGCAGATTAATGCCAGCCACACCGCAGACCCTGGAAAGTGCCAAGCCTGCCTTCAGGATGTCACTCGGAC CTTTAATGGCATCTGCAGTACCTGTTTCAAAAGGACTACAGCAGAGCCCAGCTCCAGCCTCACTTCCAGT ATCCCTGCCTCCTGTCACCAACGCTCCAAGTCTGACCCCTCACAACTCATCCAAAGTCTCACTCCACACT CTTGCCACCGGACTGGAAATGTCTCTCCTTCTGGCTGCCTCTCCCAGGCTGCACGGACTCCAGGAGACAG AGCAGGGACAAGCAAGTGCAGGAAAGCTGGCTGCATGTATTTTGGGACTCCAGAAAACAAGGGCTTTTGC ACTCTATGTTTCATCGAATACAGAGAAAATAAGCAGTCTGTTACTGCCTCTGAGAAAGCTGGTTCCCCGG CCCCCAGGTTCCAGAACAATGTCCCGTGCCTGGGCAGGGAGTGCGGCACACTCGGAAGCACCATGTTTGA AGGGTACTGTCAGAAGTGTTTCATCGAAGCTCAGAACCAGAGATTCCATGAAGCAAGAAGAACGGAAGAA CAGCTGAGATCAAGCCAGCATAGAGACATGCCTCGAACTACACAGGTAGCCTCAAGGCTGAAATGTGCCC GGGCCTCCTGCAAGAACATTCTGGCCTGTCGCAGTGAGGAACTCTGTATGGAGTGCCAGCACCTAAGCCA ACGAGTAGGTTCTGTGGCCCACCGGGGTGAGCCCACGCCTGAAGAGCCCCCTAAACAGCGCTGCCGGGCC CCTGCTTGTGATCACTTTGGCAATGCCAAGTGTAATGGTTACTGCAATGAGTGCTACCAGTTCAAGCAGA TGTATGGCTAAGTGCGAACACATTGACAGGTCCAGCAAGAAGGAGCCTCAAACTGCCATTCACAGTGGTA CTGTGATCCAGACCCCTGAGTGCCACAGGAAGGGTAGGCCCTGGGACAGTGGCTTCAAGTGTCTTTGAGC AAGTAGGCAAGATAAGCTCTTCCTTCGTCACAGCCATGACCATCAGGTTAGTAACCGAGACTTTGCCCTG GTGAGCTGAGAGTAGACCTGGTTACCGGCAGTGCATGATGTCAGGGTCAGCCTGAAGCTATTGCTGCTCG TCTACCAGGAGGATGTGGAGATCTTTAGACACGAAATGCGTGCTAGGACTGGCAGCAGTTCCTGAGCCTG GCTGCCAGGCAACAGGAAGAAAGGCGTATGGGAAAGTAGGATGGAGACTGGAACCATCCTACTGCTGCCA GCTGGAAGCTTTTCTCAGAGAGCCAAAGCTGAATTCTCAGAGCTCCAAGGCCCTCCACAAGATGCTAGAG GAAGCTCAGGGAAAAACAGGACGTAGGCGGAGCACATCTGTGGTCAATGGGCTTCCCTATCTGCCGACTT GGCTCCCAAGGTTCTGCAGGAAAGCAGACCTGCACACAGATCCCGTGGCTGCTGAAAAGACACATGTTCA CATGGAGACAAACCAAGCTGCTCGCTCTCGGCTGTATGCACCTTCTAGACAGCAGAACGTCTGGTGGGAA GACATGCCAGTGTTGGCCATTGTCTCCCATTCCAAAGAGAATAACTTTGAACTGAGGGTGTGTATCAGAG TATGTACATATGTAATATACCCTTATATTATGTATGAGGGATTTAAAATATTGAAATGGTTTCCTAGTGG TAGTAGGAAGACTGAATAAGAACAACCTATTTTCTGGTTGTTGTCAGCTTGCCCCTGTCTATACAGCTTG TTACGTGAATTCAACAAGCTGTCTTCTTAACGGGAGCCCTCATTCTGGTTTATTTATTATGAACTTCCAA GTTATTTAAATGAAGATTTCCTTCTGCAGTATTTTCTTGAGATAACATATTTGCTTTCCACCATGTCCTG GGGGCCTGGTTAGTCCCTGGCCATGGAGTGTGGTGGGATGGCTGTCTTCTCTCTCCTTGTTACCTCCCTA GGAAAGAAGGGGTTGCTTTATGGATAAGCCTGTAGAATCGGCTGCTTCCTATGACTCCCTGGCTGCACAA GAAGAAATTGTCTGTCTTCCATATATGCTTCTAAGTATCCTACCTCTTTGAAGAAAAGATAGGGATGGAG TGAGGTGACAGTGGGAAGGGACACAACTACATGGCCTTTCATGACCCTGGCTTACTGTGTAAATGCTGTG CCCTTGGGTACTGGATAGGAACACTGAGAAAGCAAAGCCAATAGCCGTGAGGACGAGGAAGTTTCTGCCA CTGCTTGCAGCTTTTCTGTGTGCTTTGGACTTGCTTTAGGAGGGAAATATAGCTTTGTAGAGATAAAGGC TGACATCTGGGGTCTAGCCTAGCACCTGAAGATACTTGTGATGTCTAACTCTGCATAGCCTTTTCTCTTC ATATCTGCGGCCTATCTTTTCCCCTTACATATGTCTTTGTCCTTCTTTCCCAAAGATGTCAAATACAATG CAGCATTTTCATCTTGTTCTTTCAAAGTTAATAGTCTATTATTTTATGTCTATTGATTTTCATTGTTAAT GTGAAATGGTTATTTATACTTATAGAAAGTTTGTGAAATTTGCACAGTTGTCTTTCAAAGGATAGGATCT ATCTTTTTTGGACTTCAGATTGTTTTAAAGAACTGTAACTTCACAAAAGTCAATATTAAAAAATAAATTA TTTAAGAACAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6843338 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6843338 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:11912 same embryo
	EMAGE:11908 same embryo
	EMAGE:11909 same embryo
	EMAGE:11913 same embryo
	EMAGE:11910 same embryo
	EurExpress:euxassay_008542 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source