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EMAGE:11984

4931431F19Rik RIKEN cDNA 4931431F19 gene ( MGI:1918230)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:11984 EMAGE:11984 EMAGE:11984 EMAGE:11984 EMAGE:11984
"Pseudo-wholemount" of euxassay_011167. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_011167_01 euxassay_011167_02 euxassay_011167_03 euxassay_011167_04
EMAGE:11984 EMAGE:11984 EMAGE:11984 EMAGE:11984 EMAGE:11984
euxassay_011167_05 euxassay_011167_06 euxassay_011167_07 euxassay_011167_08 euxassay_011167_09
EMAGE:11984 EMAGE:11984 EMAGE:11984 EMAGE:11984 EMAGE:11984
euxassay_011167_10 euxassay_011167_11 euxassay_011167_12 euxassay_011167_13 euxassay_011167_14
EMAGE:11984 EMAGE:11984 EMAGE:11984 EMAGE:11984 EMAGE:11984
euxassay_011167_15 euxassay_011167_16 euxassay_011167_17 euxassay_011167_18 euxassay_011167_19
EMAGE:11984 EMAGE:11984 EMAGE:11984 EMAGE:11984 EMAGE:11984
euxassay_011167_20 euxassay_011167_21 euxassay_011167_22 euxassay_011167_23 euxassay_011167_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:11984Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
11984_wholemount_strong.wlz
11984_wholemount_moderate.wlz
11984_wholemount_weak.wlz
11984_wholemount_possible.wlz
11984_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:11984_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
nasal cavity olfactory epithelium
moderate moderate
regionalmoderate expression: see section 08 09 10 11 16 17 18 19
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T37399
Entity Detected:4931431F19Rik, RIKEN cDNA 4931431F19 gene ( MGI:1918230)
Sequence:sense strand is shown

>T37399
AGGAAGTTCTCCAGGAACACAAGCAGAGGCTGGAGGCCCTGAAAGCCGTGCCAGGGGGTGACAACGCTAT
GCATCCCAGCTGCTCTGATATCCATCAGGTCATGCTCTCCACTCTGGCCTTATTGGTGGCTTCTAAAGGC
CACATTTCAGCCTTAGAGCTGTGTAGGGGAGAAACCAACAATGCTCACAGCAGTTCTGATCCTACCACCA
TTCCAGCCACTTCTCCTCTGGTCAGGACCTTGGCACAAGAGGTCAGTACAGGAGGAGTTAGCCAGGTCAA
GGGCATAGTTTCAAGTCAGGCCAGTTTAGGGTGCCGACCTGGTATGCTAGACTTACACTCAGGCTCAGAT
ATCCCCTGCCAAGAAAGCCAGCAGCCCTTGGAGAAAGTCCCTCTAACCTGTCAACCGAGACTCTCGCCCT
GTGTTCTGCGCCTAGCCTTGACTGTCCTGCAGCAGAATCCATCTCTGGCACATCAGTTGGCAACTGGCAG
TCCCCTACTACATCACTTGCCACTTCTCCCCATCCTCACCAACCCTCGAGCGCTGCAGGCATTGCTACAG
ATAGAGGAAGGCCTACAGATACTATCCAGGGAGGTGCCTGAGCTGGGACCATTGTTTCGGGACTCAGCTA
AACCACGTGGG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 80628. Forward Primer - name:080628_F_cDNA_4931431F19Rik, sequence:AGGAAGTTCTCCAGGAACACAA; Reverse Primer - name:080628_N_SP6_cDNA_4931431F19Rik, sequence:CCCACGTGGTTTAGCTGAGTC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:11987 same embryo
 EMAGE:11985 same embryo
 EMAGE:11983 same embryo
 EMAGE:11982 same embryo
 EMAGE:11986 same embryo
 EurExpress:euxassay_011167 same experiment
 MGI:4822749 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS