Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:12021

Cyp2b19 cytochrome P450, family 2, subfamily b, polypeptide 19 ( MGI:107303)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:12021 EMAGE:12021 EMAGE:12021 EMAGE:12021 EMAGE:12021
"Pseudo-wholemount" of euxassay_009550. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_009550_01 euxassay_009550_02 euxassay_009550_03 euxassay_009550_04
EMAGE:12021 EMAGE:12021 EMAGE:12021 EMAGE:12021 EMAGE:12021
euxassay_009550_05 euxassay_009550_06 euxassay_009550_07 euxassay_009550_08 euxassay_009550_09
EMAGE:12021 EMAGE:12021 EMAGE:12021 EMAGE:12021 EMAGE:12021
euxassay_009550_10 euxassay_009550_11 euxassay_009550_12 euxassay_009550_13 euxassay_009550_14
EMAGE:12021 EMAGE:12021 EMAGE:12021 EMAGE:12021 EMAGE:12021
euxassay_009550_15 euxassay_009550_16 euxassay_009550_17 euxassay_009550_18 euxassay_009550_19
EMAGE:12021 EMAGE:12021 EMAGE:12021 EMAGE:12021 EMAGE:12021
euxassay_009550_20 euxassay_009550_21 euxassay_009550_22 euxassay_009550_23 euxassay_009550_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:12021Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
12021_wholemount_strong.wlz
12021_wholemount_moderate.wlz
12021_wholemount_weak.wlz
12021_wholemount_possible.wlz
12021_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:12021_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
left lung
strong strong
regionalstrong expression: see section 04 05 06 07 08 09 10 11 12 13
right lung
strong strong
regionalstrong expression: see section 13 14 15 16 17 18 19 20 21 22 23 24
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T36222
Entity Detected:Cyp2b19, cytochrome P450, family 2, subfamily b, polypeptide 19 ( MGI:107303)
Sequence:sense strand is shown

>T36222
CTCTCTCTTGAGTTGGCTCCATCATGCTCCCACGTGTTGTGTTGGAGGGTCATGTTTGAGAATCCCCTTT
ATAGATGAGTGACAGGCCACATGATGACATCACACTGCTAGTGAGTAGCGGGGGACAAGATCACACTGTG
AGTCTAGAAAAGCACCTCCCCTCCCCTTCCCTACTTGTGCTTAAATTGAGATGAAATTCATAAAATGAAG
CTGACAAAATTTTTCAGTTACGTATTCTGAGACAGAGTCTCATGTAGCTCAGACCAACCTCAAAATCCTA
TATACATCCAAAGATGTCTTGAATTCTGGATCTGTTTTCCACTACTGGCATCATAGGCACATGGACCTAC
ACCCAGATTTTAAACTGACTATATCTTAATGTTTAGATTATAATGTCTTTTAATTTATTCACAATGCTGT
ATGATTTCACTACCTAATTCCAGGTATTTTGTCACATTTAACCAACACCACCCCAAACCCTGACCTATTA
AAAGATCAGTCCCTCGTCCCTCTCTCCATAACCTCCCCCACATCTACCACCTACCCTGCCACACACAGTC
TGACTTCTGTGTCAATGGACTGACCTGACTCCATGTGTCTTGTAGAAAAAGCAACAATGTGATCTTTTGT
CAGCTGTCCCTGCTTCTCAGGTCCCTGTACTGTGCGGGCCACAGTCACACACCTTCACAGACTTTCCCAT
TCTCTTTCTTAGACAAGGATCTCCTCAGCCCTCAGGCCCTGAACTGAATGACTCTGCTTAGATTCTCCCA
CATTCCCCTG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 97614. Forward Primer - name:097614_F_cDNA_Cyp2b19, sequence:CTCTCTCTTGAGTTGGCTCCAT; Reverse Primer - name:097614_N_SP6_cDNA_Cyp2b19, sequence:CAGGGGAATGTGGGAGAATCTA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:12020 same embryo
 EurExpress:euxassay_009550 same experiment
 MGI:4824163 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS