EMAGE mouse gene expression spatial database

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EMAGE:12033

Snapc4 small nuclear RNA activating complex, polypeptide 4 ( MGI:2443935)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008276. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008276_01	euxassay_008276_02	euxassay_008276_03	euxassay_008276_04

euxassay_008276_05	euxassay_008276_06	euxassay_008276_07	euxassay_008276_08	euxassay_008276_09

euxassay_008276_10	euxassay_008276_11	euxassay_008276_12	euxassay_008276_13	euxassay_008276_14

euxassay_008276_15	euxassay_008276_16	euxassay_008276_17	euxassay_008276_18	euxassay_008276_19

euxassay_008276_20	euxassay_008276_21	euxassay_008276_22	euxassay_008276_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12033_wholemount_strong.wlz

12033_wholemount_moderate.wlz

12033_wholemount_weak.wlz

12033_wholemount_possible.wlz

12033_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12033_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9228
Entity Detected:	Snapc4, small nuclear RNA activating complex, polypeptide 4 ( MGI:2443935)
Sequence:	sense strand is shown >T9228 AGAAGCATGATCTGCGTGTGGTCGCCCGGGCGATGATGCCAGCTGTGAAGTTTGGAAGGGATGCCTAGAA GGCAGGCTGAAGCCATGGACATAGACGCCGAACGAGAGAAAATAACACAGGAGATCCAGGAGCTTGAAAG GATTTTGTATCCTGGATCCACCAGCGTCCACTTTGAAGTCTCTGAATCCAGTCTCAGTTCAGACTCTGAA GCAGCCTGGGACCCTCTAAGCAATGGTGAATCCTGGTGGGGAGAAGAGGACCCAGAGCTCAGCCTATCAA CTTGAATAGTCTCCATGGCCCAGATACACAGAATTTCGTCCCTGAGCACTCCAATTCGCTGCCTGATGAG GACTTGGAAACTGCTGGTGCCCCCATCTTGGAAGAGGAAGGGTCGAGTGAGAGCAGCAATGATGAGGAGG ACCCCAAGGACAAAGCTCTCCCTGAAGACCCCGAGACCTGTCTGCAGCTAAATATGGTCTACCAGGAGGT GATCCGGGAAAAGCTAGCAGAGGTTAGCCAGTTGCTAGCCCAGAACCAGGAGCAGCAGGAAGAGATCCTG TTTGATCTGTCTGGAACCAAATGCCCCAAGGTGAAGGATGGCAGAAGTCTGCCCTCATACATGTACATAG GCCACTTCCTGAAGCCATACTTCAAGGACAAGGTTACTGGAGTGGGGCCTCCTGCCAACGAAGAAACACG GGAGAAGGCTACCCAGGGAATCAAGGCCTTTGAACAGCTCTTGGTGACCAAGTGGAAGCACTGGGAGAAA GCCTTGCTCAGGAAGTCTGTAGTGAGTGACCGCCTACAACGTCTGCTTCAGCCCAAATTGCTGAAGCTGG AGTACTTACACGAAAAACAGAGCCGAGTCTCCAGTGAGTTGGAAAGGCAGGCCCTGGAGAAGCAGATTAA GGAAGCAGAGAAGGAGATTCAGGACATCAACCAGCTCCCAGAGGAAGCATTGTTGGGGAACCGGCTAGAC AGCCACGACTGGGAGAAGATTTCCAATATTAACTTTGAAGGAGCCCGCAGTGCAGAGGAGATCCGGAAGT TCTGGCAGAGTTCTGAGCATCCAAGCATCAGTAAGCAGGAATGGAGCACAGAAGAAGTAGAGAGGCTGAA GGCCATCGCTGCCACACATGGCCACCTGGAGTGGCACTTGGTTGCAGAGGAACTTGGGACAAGCCGCAGT GCCTTTCAGTGCCTGCAGAAATTCCAGCAGTACAACAAAACCCTGAAACGCAAGGAGTGGACAGAAGAGG AAGACCACATGCTCACCCAGCTGGTCCAAGAGATGCGAGTTGGGAACCATATCCCATACCGCAAGATCGT CTACTTCATGGAAGGGAGAGACTCCATGCAGCTGATCTACCGTTGGACCAAGAGCTTAGATCCCAGCCTG AAAAGGGGATTCTGGGCCCCAGAGGAAGATGCTAAATTGCTTCAAGCTGTTGCCAAGTATGGGGCGCAGG ACTGGTTTAAAATCCGGGAAGAGGTGCCAGGTAGGAGCGATGCCCAATGCAGAGACCGCTACATCAGAAG ATTACATTTCAGCTTGAAAAAGGGACGGTGGAATGCAAAAGAAGAACAACAGCTGATCCAGCTAATAGAG AAATATGGTGTTGGTCACTGGGCAAGAATCGCTTCTGAATTGCCCCATCGGTCAGGCTCCCAGTGTCTGA GCAAGTGGAAGATCTTGGCCAGGAAGAAACAGCATCTGCAGAGGAAGAGAGGGCAGAGGCCCCGTCACAG TAGCCAGTGGAGCTCTAGCGGCAGCAGCAGCAGCAGCAGTGAAGACTACGGCAGCAGCAGTGGCAGCGAC GGCAGCAGCGGCAGTGAAAACTCAGATGTGGAGCTGGAGGCGTCTCTGGAGAAGAGTAGAGCACTGACTC CTCAACAGTACAGAGTGCCAGACATTGACCTGTGGGTTCCTACCAGGCTGATCACCAGCCAGTCACAGAG AGAAGGGACAGGGTGCTACCCACAACACCCTGCTGTCTCCTGCTGCACTCAGGATGCCTCTCAAAATCAC CACAAGGAAGGTTCTACCACCGTCTCTGCTGCTGAGAAAAACCAGCTGCAGGTGCCCTATGAGACCCACA GCACTGTTCCAAGAGGAGACCGGTTCCTACACTTCTCGGACACCCACTCAGCAAGTTTGAAGGATCCGGC CTGCAAGAGTCACACACTGATGAAGGAAAGACCGAAGCAGCCACTCCTCCCTAGTTCACGCTCAGGGTCT GACCCTGGCAACAATACGGCTGGGCCCCATCTGCGGCAGCTATGGCATGGAACTTACCAGAATAAGCAGA GACGCAAGAGACAGGCTCTACATCGCAGGCTCCTTAAGCACAGGCTTTTGCTGGCTGTGATACCCTGGGT GGGTGACATCAACCTGGCCTGCACACAAGCTCCCCGGAGACCTGCAACCGTTCAGACTAAAGCTGACAGC ATCCGGATGCAGTTAGAGTGTGCCCGCCTGGCCAGCACTCCGGTATTCACACTGCTTATTCAGCTATTGC AGATTGATACTGCGGGCTGCATGGAGGTGGTTCGAGAGAGGAAAAGCCAGCCCCCTGCACTGCTCCAGCC TGGCACCCGGAACACCCAGCCGCATCTCCTGCAGGCATCCTCTAATGCCAAGAACAACACAGGCTGCCTC CCAAGCATGACGGGGGAACAAACTGCAAAGAGAGCCAGCCACAAAGGGCGCCCACGACTGGGGTCATGCA GGACTGAGGCTACCCCCTTCCAAGTCCCTGTAGCAGCCCCACGTGGCCTCCGGCCAAAGCCTAAGACTGT TTCTGAGCTGCTTCGGGAGAAGCGGCTCCGTGAGTCCCATGCCAAGAAGGCCACTCAGGCTCTTGGTCTC AACAGCCAGCTGCTGGTCTCCTCACCTGTGATCCTCCAGCCCCCTCTGCTGCCAGTCCCCCATGGTTCCC CAGTAGTAGGCCCTGCAACGTCCAGTGTAGAACTCTCTGTACCTGTGGCGCCGGTGATGGTCAGTTCAAG TCCTTCTGGCTCCTGGCCGGTGGGTGGGATCTCAGCCACAGACAAGCAGCCCCCCAACCTACAGACCATT TCCCTAAATCCTCCCCACAAAGGGACCCAGGTTGCAGCCCCTGCTGCTTTTAGGAGCCTAGCCTTAGCCC CCGGGCAAGTCCCCACAGGCGGCCATCTGAGTACTCTAGGACAGACCTCTACCACATCCCAGAAGCAGAG CCTGCCCAAGGTCCTGCCCATTCTTCGAGCTGCTCCCAGCCTCACTCAGCTGTCTGTGCAGCCACCAGTA AGTGGACAGCCCCTGGCAACCAAGTCCAGCCTGCCTGTCAACTGGGTTCTCACAACTCAGAAGTTGCTCT CTGTCCAAGTGCCAGCTGTGGTGGGCCTCCCCCAGTCAGTGATGACCCCTGAGACCATAGGACTACAAGC AAAACAGCTGCCTTCCCCTGCCAAGACTCCTGCTTTTCTGGAACAGCCTCCAGCCAGTACAGACACAGAA CCCAAGGGACCTCAGGGCCAGGAAATACCACCCACACCTGGGCCCGAAAAGGCAGCTTTGGACCTAAGTC TCCTTTCCCAGGAAAGTGAGGCAGCCATCGTCACATGGCTGAAGGGGTGCCAAGGTGCTTTTGTACCCCC GCTGGGGAGCAGGATGCCCTATCATCCCCCTTCCCTGTGCAGCTTGCGGGCATTGTCCAGCCTCCTCCTC CAGAAACAGGACTTGGAGCAGAAGGCTTCCTCTCTGGCGGCCAGCCAGGCAGCTGGGGCCCAACCTGATC CCAAGGCTGGAGCCCTACAAGCTTCCCTGGAGCTGGTACAGAGGCAATTCCGGGACAACCCAGCCTACCT CCTGCTAAAGACGCGTTTCCTGGCTATCTTCTCCCTTCCTGCATTCCTGGCCACTCTGCCCCCCAACAGC ATCCCCACCACCCTGTCACCAGACGTGGCTGTGGTTTCTGAGAGTGACAGTGAAGACCTCGGTGACTTGG AGCTCAAGGATAGGGCTAGACAGTTGGACTGCATGGCCTGCAGAGTGCAAGCAAGCCCAGCAGCCCCAGA CCCTGTACAGAGAGCTCCAAGTCCTGGTGAGGTTTCTGCTCCCTCCCCTCTGGATGCTTCTGATGGCCTT GATGACCTTAACGTGCTCAGGACTCGACGTGCTCGGCATTCCCGGAGATAGAGGTTGCTTTGAGCAGCAA GGACAAGGCGTCAAGGGGTCCAGAATCCAGCTCTCACTAGGCAAGAGGCAGGGCCCAAGCCTGCCCAGAA GCAGTACACAACCCTCAGCAAGAAGACGGGAAGCCAGAAAGCTGCAGGCTGAGGACCAAGCTGTCGGTAG CGATCTACAGGCTGTGGTGATAGCAGTGGCCACGTGATCACAGGCTACCTGTTAACAGACTTGGGGCCGT GTATGTGAGGGAATAAAGGAAATGCCTAATTTTCACTTAAAAAGCAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6833968 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6833968 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12035 same embryo
	EMAGE:12037 same embryo
	EMAGE:12036 same embryo
	EMAGE:12034 same embryo
	EMAGE:12038 same embryo
	EurExpress:euxassay_008276 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source