EMAGE mouse gene expression spatial database

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EMAGE:12049

Gabrb3 gamma-aminobutyric acid (GABA) A receptor, subunit beta 3 ( MGI:95621)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008367. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008367_01	euxassay_008367_02	euxassay_008367_03	euxassay_008367_04

euxassay_008367_05	euxassay_008367_06	euxassay_008367_07	euxassay_008367_08	euxassay_008367_09

euxassay_008367_10	euxassay_008367_11	euxassay_008367_12	euxassay_008367_13	euxassay_008367_14

euxassay_008367_15	euxassay_008367_16	euxassay_008367_17	euxassay_008367_18	euxassay_008367_19

euxassay_008367_20	euxassay_008367_21	euxassay_008367_22	euxassay_008367_23	euxassay_008367_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12049_wholemount_strong.wlz

12049_wholemount_moderate.wlz

12049_wholemount_weak.wlz

12049_wholemount_possible.wlz

12049_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12049_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

brain

strong

regional

strong expression: see section 01 02 03 04 05 06 07 09 10 11 12 13 14 15 17 18 19 20 21 22 23 24 moderate expression: see section 08 16

facial vii ganglion

strong

regional

strong expression: see section 04 05 20 21

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 18 moderate expression: see section 06 07 08

trigeminal v ganglion

strong

regional

strong expression: see section 02 03 04 05 17 18 19 20 21 22 moderate expression: see section 06 07 08

vagus x ganglion

strong

regional

strong expression: see section 17 moderate expression: see section 09

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 17 18 moderate expression: see section 07

neural retina

moderate

regional

moderate expression: see section 01 02 22 23 weak expression: see section 24

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 09 11 14 15 16

mandible

moderate

regional

moderate expression: see section 02 22 23 weak expression: see section 03 04 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T35166
Entity Detected:	Gabrb3, gamma-aminobutyric acid (GABA) A receptor, subunit beta 3 ( MGI:95621)
Sequence:	sense strand is shown >T35166 ATGTGAATGCCATAGACAGGTGGTCCCGCATCGTGTTTCCATTCACCTTTTCTCTCTTCAATTTAGTTTA CTGGCTGTACTATGTTAACTGAGTGACTGTACTTGATTTTTCAAAGACTTCATTTAACACTGAGTGAAAT ATTACCCTGCCTGTCAAGTTTTTATACCAGTACACATACAAACACACACACACATACGCACACACACACA CACGGACACACACACAATTGTGTATATATATGTGAACTTTCTCAGCATATATATAAAATACACGTGTATA TGAGAATGTATGTGTATATGTTCAAGGACACAGATAAGAGTATCTGTGTACATAAAACAAATACGCATAC ATACCATACATTTTGCAACTATGGACAATTTAACACAGGATGCATATTAAAGAAACTCATAGCTTTTTCT TTCTTTTTTTAATTGAAAGGGACAAGTATCTAAATATTATGCCTCAAGAATGAGGGCGTGAAACACAGTC ATCCCAAAGTGTGTCTTTTATTATCATAAGTTAGATATTTTCGCTTAAAAATCCAAAAGGAATTCTTAGT TAATCTTTGGGAACTCCCACAGCCGTAGTTGTACACCTCATACCCTCTCAGTTTGCAACCGAGGCTTGTT GGCTTCTCTGTGATGGGCTTGTTTCATCAGGTGTGTTTTTCTGTAGTGGGTTAGCATATGAGATGAGTCA CTGGCAGTGCACTTACCTGACTCTTACTGGTAGGTAGCTCCTACGCTGGTGTCTGAATAGTCATCTTGAA AAACTCACTGGGAATAAACGGTGTCCCGTTTTAAGTACTCCACCATCCCATATACATGG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 94254. Forward Primer - name:094254_F_cDNA_Gabrb3, sequence:ATGTGAATGCCATAGACAGGTG; Reverse Primer - name:094254_N_SP6_cDNA_Gabrb3, sequence:CCATGTATATGGGATGGTGGA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12046 same embryo
	EMAGE:12045 same embryo
	EMAGE:12047 same embryo
	EMAGE:12048 same embryo
	EurExpress:euxassay_008367 same experiment
	MGI:4824965 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source