EMAGE mouse gene expression spatial database

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EMAGE:12054

Arhgef15 Rho guanine nucleotide exchange factor (GEF) 15 ( MGI:3045246)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_009548. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_009548_01	euxassay_009548_02	euxassay_009548_03	euxassay_009548_04

euxassay_009548_05	euxassay_009548_06	euxassay_009548_07	euxassay_009548_08	euxassay_009548_09

euxassay_009548_10	euxassay_009548_11	euxassay_009548_12	euxassay_009548_13	euxassay_009548_14

euxassay_009548_15	euxassay_009548_16	euxassay_009548_17	euxassay_009548_18	euxassay_009548_19

euxassay_009548_20	euxassay_009548_21	euxassay_009548_22	euxassay_009548_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12054_wholemount_strong.wlz

12054_wholemount_moderate.wlz

12054_wholemount_weak.wlz

12054_wholemount_possible.wlz

12054_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12054_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

other

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9311
Entity Detected:	Arhgef15, Rho guanine nucleotide exchange factor (GEF) 15 ( MGI:3045246)
Sequence:	sense strand is shown >T9311 AGGGAACTGGAGGGGGATGACTTCCGCAGAACACCCCACTCCTTGGAAACACTCAGAAGACGATGTCTGC ACAGTCTCTTCCTGCAGCAACGCCCCCTACCTTGAAGCCCCCTCGGATCATCCGCCCCCGACCCCCATCT CGGCATAGGGCTCCACACTCCCCGGGGCCTCTCCACAATGGCTCCTCTCCGAAAGCACTTCCTCAAATAT CCAATGATGCATCGGCCTCAGTGTGCACCTCCATCTTCTGGGAGCCCCCCACGGCTTCCCTGAAGCCCCC TGCTCTTCTGCCTCCCTCAGTTTCTAGAACCAGCCTGGACTCTCAGACTTCCCCTGACTCACCTTCCAGC ACACCGAGTCCCAGCCCAGTGTCCCGGCGCTCCATCTCCCCAGAACCAGCTCCCTGCTCTCCGGTGCCCC CACCCAAGCCCTCTGGGTCTTCCCGAACACCTCTGCCCTCGGGCCCTACGCCTCTCCAGGATGGCTCTGC TTCGGCCCCTGGCACGGTTCGTAGACTGGCCGGCAAGTTTGAATGGGGAGCTGAAGGCAAAGCCCAGTCC TCAGACTCCCTGGAACGGTGTTCCCAAGGGAGCACGGAAGTGAATGGGGAGAAAGAGACTCCCGAAGCCG CCCTTTCTGGGAATGGGTCCCAGGAGAACGGCACTCCCGATGCTGCCCTGGCCTGCCCTCCATGCTGCCC CTGTGTCTGCCATGTAGCAAAGCCTGGCCTGGAGCTCCGGTGGGTGCCTGTGGGGAGTTCTGAGGACATC CTCAGGATCCCCTGCCGGGCATCCCCACTCCGAGCCTCCCGCTCCCGCATCAATCCTCCAGTCATCAGTC ACCCTCCAGTTGTGCTCACGTCCTATCGCTCCACTGCCGAGCGCAAACTCCTACCTCCCCTCAAGCCCCC CAAGCCAACTAAAGTCAGACAGGATATCAGCACCTCTGAGGAGCTTCCACAGCCAGATCTGAAACTGCCT TCAGAAGATGGGATCCAAACTGCTACAAAGGCCTGGGAAGGGGACAGACCTGAAGGAGCTCCTCTGAATG CTCCCCCTGTAGCCTTGGAGGGAAGGGAGGAGGAAGGATTGGATGGGCTGAAGGGGCTGCAGTGGGAACT GCCCCTGCAGGACGAACCTTTATACCAGACTTACCGAGCAGCTGTGCTGTCAGAGGAGCTGTGGGGTGTG GGTGAGGATGGGGGTCCCTCTCCAGCTAATCCTGGAGAAGCTCCCACCTTCTCCAGGCTCCCTGGACCTC GAAACACTCTGTGGCAGGAGCTCCCGGCTGTGAGAGGCAGTGGCCTCTTAGAGAGTCTAAGCCCCCAGGA GCGGCGCATGCAGGAGAGCCTGTTTGAGGTGGTGACATCAGAGGCCTCCTACCTGCGATCCCTGAGGCTG CTGACCGACACCTTCGTCCTGAGCCAGGCTCTCCGGGACACACTCACGCCTCGGGATCATCATACGCTGT TCTCCAACGTGCAGCGAGTCCAGAGCGTCAGTGAGCGGTTTCTAGGAACACTGCTGTCTCGTGTTCGTTC CTCCCCACACATCACTGACCTGTGTGATGTGGTGCATGCCCATGCAGTTGGTCCTTTCTTCGTGTATGTG GATTATGTTCGGAACCAGCAGTATCAGGAGGAGACCTATAGCCGCCTTATGGATACAAACGTGCGCTTCT CCGCAGAGCTGCGAAGGCTGCAGAGCCTCCCCAAATGTGAGCGGCTCCCGCTGCCATCTTTCCTGCTGCT GCCCTTCCAGCGCATCACCCGGCTGCGGATGCTGTTGCAGAATATCCTAAGCCAGACAGAAGAGGGGTCC AGTCGTCAGGAGAACGCCCAGAAGGCTCTGGGTGCTGTGAGCAAGATCATTGAGCGCTGCAGCGCTGAGG TGGGGCGCATGAAGCAGACGGAAGAGCTGATACGGCTCACCCAAAGGTTGCGCTTCCACAAAGTCAAGGC TCTACCCCTGGTCTCCTGGTCACGGCGCCTGGAATTGCAGGGGGAGCTGACCGAGCTGGGGTGTCGGAGG GGGGGTGTGCTCTTCACCTCCCGTCCACGCTTCACTCCCCTCTGCCTACTGCTCTTCAGTGACCTGCTGC TTATCACTCAGCCCAAGAGTGGGCAGCGGCTGCAGGTTCTGGACTATGCCCATCGCTCCCTGGTGCAGGC CCAGCAGGTTCCTGACCCATCTGGACCCCCTACTTTCCGCCTCTCGCTTCTCAGCAACCACCAGGGCCGC CCCACCCACAGGCTACTCCAAGCGGCCTCACTATCAGACATGCAGCGCTGGCTGGGAGCCTTCCCCACCC CAGGTCCCCTTCCCTGTTCCCCAGACACCATCTATGAGGACTGTGAGTGTTCCCAGGAGCTATGTTCAGA GCCATCCACACCTTCCAAGACTGAGGGGCAAAGTCTGGAGTCCAAGGCCCCCCGAAAGCATCTGCACAAG AATCCAGAAGGTTGGCTTAAGGGGCTTCCTGGAGCCTTCCCTGCCCAACTGGTGTGTGAAGTCACAGGAG AACACGAGAGGAGGAAGCATCTTCGCCAGCACCAGAAGCTCCTTGAGGCTGTAGGACCCTCTTCAGGCAC TCCTGACACCCCTCAACCTTAAGGCAGGCTAGGGAGGAGCACAGATTGGGATACTGGCAGCAGCATGGGG AAGAAAAAGTCTATCCATCCATCTGTCCAACCGTCCAACCGTCCATCCATCCATCCATCCATCTACTTGC TGTCAGTGGAAACACTACCTCTAGAGGCAACCAATAGGGATAGAGCAGCTAATGAAACAAGACCATCTGA GCCCACGACAAGGAAGAATGGGGATGGCTCGAATGTGTTGCCAATGGAGACAGGGGATGAGTACAGAGTG GCCAAGGCTGAGGCCACGGCCAGAGGGTATCAAAGTATGTCCACAACCAAGGAAGATTAAGCAGGGGCAT TGCATTGAGAAATGACGTCATTGAAGTAGTCCTGTAGACAACAGGGATGGGATGGGACCTATCCAGCCAG AGAAGACGGTGATTTGGGCTCAAGAGAGCAGTAGTCACCCTTGTTGGTTTTACAGAAGCCACTGGTCCTG CTTTTTGAGTATAGGAAGTATTAAATTAATCTAATACTTCTGTTTGAGGGCTACTTTCTTTGCCCATTTT AAACCACTTCCTTTCTTCTAGAAGAATCCAGAGTGCATCTCAGAGAATATGAGTGCATGTGTGTTCATGT ATATATGTATCTAGGAAGGCAACTGTGCTGAATGTGGTGTGTGTGTGTGTGTGTGTGTGTGAGAGAGAGA GAGAGAGAGAGAGAGAGAGAGAGAGAACGCACCTCTCCCCTCTCCTGTTGGTGACTGAATACTTCCTTTC CTCTATCATCTCACATTTATCCTCTTATTTTCTCCAAAAAAAGCCATATGCCCTGAGATCTAAAAAAAGA GTACTGAGCATAGCTTGCCCAAGGACTGGGACCGTGAATGCAAGCCATGGATTCAGGGTCTCCCGGGTGT CTCTGGGTTCTATGACAGTTGAAAGCTGGTATTATGAAGCAGCTGGTGGCCCGCAAGGAAGGCACTGAGG ACTAAGCCACTGGGATTTGAGATCCTCAGCAGTCTGTGGACCTCTTCCTGTCCCAGGCATTGACCTCTGT GATCCATGTCTGCTCTGTGTCATCTCCCGACTTGAATTGTCATCATGATGTCAGCCAGCCTCGGGCTGCA GAACAAGTTGGGTCCTGGGTCAGCTGGCAACCATCCTGATCTTCTTCTATCCTGATCCTCTCAGAAGCAG AAGGCTGAACCCAGGAGGGAGTAGAGAGCTGATGGTGTTACTCAGACTCAACCACGGTTTCTGCTAGAGA ACCTGCCCTGTGTGATGCTGGAGCGGTGAAGGCAAAGGCAGTAGAATAGACAATTGCTGGGCTTGGGGCC CAGACCTGGGCAAAGGCTTTCTAAATCTTCAGGACTGTGCAATACTGTTCCTTCATGTATGACAACCTGC CCAGATGGAGACTGCTTAGGTGACTATGGGAGGGGTAATGAGTCTGGGGACTTGAAGGCAATAGGGCACT TTGTGTGTCCACAGCTTTCATCCAGCAAAGTGCCAGCCACATGGGAAACCCTCAATAAATGTTGCTGGAA AGAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6844583 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6844583 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12050 same embryo
	EMAGE:12053 same embryo
	EMAGE:12051 same embryo
	EMAGE:12052 same embryo
	EurExpress:euxassay_009548 same experiment
	MGI:4823230 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source