EMAGE mouse gene expression spatial database

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EMAGE:12070

Arap3 ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 3 ( MGI:2147274)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008606. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008606_01	euxassay_008606_02	euxassay_008606_03	euxassay_008606_04

euxassay_008606_05	euxassay_008606_06	euxassay_008606_07	euxassay_008606_08	euxassay_008606_09

euxassay_008606_10	euxassay_008606_11	euxassay_008606_12	euxassay_008606_13	euxassay_008606_14

euxassay_008606_15	euxassay_008606_16	euxassay_008606_17	euxassay_008606_18	euxassay_008606_19

euxassay_008606_20	euxassay_008606_21	euxassay_008606_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12070_wholemount_strong.wlz

12070_wholemount_moderate.wlz

12070_wholemount_weak.wlz

12070_wholemount_possible.wlz

12070_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12070_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

other

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9397
Entity Detected:	Arap3, ArfGAP with RhoGAP domain, ankyrin repeat and PH domain 3 ( MGI:2147274)
Sequence:	sense strand is shown >T9397 CCACCCTGAACCTCTGCTCTGGAAACCAGTCAATGGCCTGAAAGCCCATGGCTGCCCCTCAGGACCTGGA CATCGCTGTGTGGTTGGCCCTGGTGCATCTGGAGCAGTATGCGGACACTTTCCGACGGCATGGCCTAGCT ACAGCTGGTGCGGCCCAACACCTGGGCCACGAGGAGCTGAGGCACTTGGGCATCAGTGCTACAGGACACC GGAAACGCATCTTGCGCCTGTTGCGGGCAGGCTCTGCTGAGGGTTTCCTGGATTCTCATCTGGATAATAC CATGGAGCCAACTCCCAGCCCAGCCCCTGATGCCCAACCCCCCAAGCCTGTGCCTAAGCCCAGGACGGTG TTTGGGCTCAGCAACCCTGCCACTGCCCAGAGGCCTGGACTGAGCCCAATCTTCTGGGATCCAGAAGTAT CCAGGAACTCAGAGTGTACGCAAAGATCCTCTCCTCTCCTTCCTTCCTCCTCTGAACATCCTTCAGTCCC GAATACCATGGAGATGATGCCTAATGCCATCTACTTTGGCCTGGACTTAAGAGGTCGGGCCCAGGCAGCT CAGGACGTGACTCCAGATAGTTCTCAGGCGACTGTCCCAACCCCTGCCTTCAGGCCCACAACAGGCACAG TGCACATCATGGATCCTGGTTGCCTGTACTACGGTGTCCAGCCTGTGGGGATCCCAGGAGCCTCTGACAG GAGAGACGGCAGGGGTGTCTGTCAGGAAAGGGCAGAACACAGACAGGATCTGGAGACACGGGAGGATGCT GGTTATGCCAGCCTCGAGCTACCTGGAGACTCCATTCTTTCGCTACCCACCCAGGATGCAGAGACCAGTG ATGACCTCATTTCGCCCTATGCCAGCTTCTCCTCCACAGCAGATCGTCCCGTGCCTCTGCTCAGTGGCTG GCTGGACAAGCTCTCACCTCAGGGAAACTATGTGTTCCAGAGACGTTTTGTGCAGTTCAATGGAAGGAGT CTAATGTACTTTGGCAGTGACAAGGACCCCTTCCCCAAAGGTGTGATCCCTCTGACTGCCATCGAGATGA CCCGAAGCAGCAAGGACAACAAATTCCAGGTCATTACCGGCCAGAGAGTATTTGTGTTCCGCACAGAGAG TGAGGCTCAGCGTGACCTATGGTGCTCCACGCTGCAATCCTGCCTGAAGGAACAGCGCCTCCTGGGCCAC CCCCGGCCCCCTCACCCACCACGACCCCTCCGCACGGGCACGTTAGAGTTGCGTGGACACAAGGCCAAGG TGTTTGCTGCCTTGATCCCTGGAGAGCTGGCACTGTACAAGAGTGAGCAGGCCTTCTCACTGGGCATCGG GATCTGCTTCATTGAACTGCAAGGCTGCAGTGTCCGGGAGACCAAGAGTCGAAGCTTCGATCTGCTCACA CCCCATCGCTGCTTCAGCTTCACAGCCGAGTCTGGGGGGGCTCGACAGAGCTGGGCAGCCGCTCTGCAAG AAGCAGTAACCGAGACACTGTCTGACTACGAGGTGGCTGAGAAGGTCTGGTCCAACCCGGCAAACCGGCA TTGTGCGGACTGCAGGGCCTCCCGGCCGGATTGGGCTGCCGTCAACCTGGGGGTCGTCATCTGCAAGCAG TGTGCAGGTCAGCACAGGGCCCTGGGCTCTGGGATCTCCAAGGTGCAGAGCCTGAAGCTGGACACGAGTG TCTGGAGTAATGAGATAGTGCAGCTGTTCATTGTCCTGGGAAATGATCGTGCCAATTGCTTCTGGGCAGG GGCACTTCCCCCAGGTGAAGGGCTGCATCCAGATTCAGCCCCTGGCCCTCGAGGAGAGTTCATCTCCCGA AAGTATAAGCTGGGTCTCTTCCGGAAGCCCCACCCTCGGCATCCAGATCATAGCCAGCTTCTCCAGGCAC TGTGTGCAGCTATGGCAGGACCCAACCTGCTCAAGAACATGGCCCAGCTTCTCTGTGTCGAAACCTCTGA GGGAGAGGAACCCTTGTCCCCTTCAGCCCTCAATGGCAGCTTGCTTAGCCTTCTACCTTCAGACTCCCCT GGAGTATACAATGAGGTGGTAGTGCCTGCCACTTACAGAGGTTTCCTGTACTGTGGCTCCATCAGCAACA AGGCTGGAGCCCCACCCCTTCGCAGGGGCCGGGATGCTCCTCCCCGCTTGTGGTGTGTGCTGGGAGCTGC CCTGGAAATGTTTGCATCAGAGAGCAGCCCTGAACCCCTCAGCCTTCTCCAGCCCCAGGATATTGTATGT CTGGGTGTAAGCCCCCCACCTGCTGATCCAGGTGACCTTGACAGGTTCCCCTTTTCCTTTGAACTCATCC TCACTGGGGGAAGAATCCAGCATTTTGCCACTGATGGAGCAGACAGTCTAGAGGCCTGGATCAGTGCAGT GGGCAAGTGGTTCTCCCCGCTCAGCTGCCACCAGCTGCTGGGCCCTGGGTTGCTGCGGATGGGTCGCCTG TGGCTACGGTCCCCCTCCCACGCAGGCCTGGCCCCTGGTCTCTGGCTGTCGGGCTTCGGCCTCCTTCGTG GTGATCATCTCTTCCTGTGCCCAGCGCCTGGCCCTGGTCCCCCTGCTCCGGAGGACATGGTGCATTTACG ACGTCTACAGGAGATCAGTGTGGTCTCGGCAGCTGACACTCCAGACAAAAAGGAGCATTTGGTCCTTGTA GAGACAGGAAGGACCCTGTATTTGCAGGGGGAAGGCAGGCTAGACTTTGCAGCGTGGAACACGGCCATTG GAGGTGCTGCCGGAGGGGGTGGCACGGGCCTGCAGGAGCAGCAGATGAGCCGGGGAGACATCCCTATCAT TGTGGACGCTTGCATCAGTTTTGTCACGCAGCATGGGCTCCGGCTGGAAGGTGTATATCGGAAAGGAGGC GCTCGAGCCCGCAGCCTGCGACTCTTGGCTGAGTTTAGGCGGGATGCCCGGTCAGTGAAGCTTAGACCAC GGGAACACTTTGTAGAAGATGTTACTGACACACTCAAACGCTTCTTTCGAGAGCTTGATGACCCCGTGAC CTCTGCTAGGTTGCTGCCTCGATGGAGAGAAGCTGCAGAGCTTTCCCAGAAGAATCAGCGCCTGGAGAAG TACAAAGAAGTGATTAGCTGCCTGCCTCGAGTCAACCGCAGAACACTGGCCACCCTCATCGGGCATCTGT ACCGGGTGCAGAAGTGTGCGTCTCTAAACCAGATGTGCACGCGGAACCTGGCCCTACTGTTTGCACCTAG TGTGTTCCAGACAGATGGGCGGGGAGAGCATGAGGTTCGGGTACTGCAGGAGCTCATCGATGGCTACATC TCTGTTTTTGATATTGACTCGGACCAGGCAGCTCAGATTGACTTGGAGGTCAGTCTCATTACCACCTGGA AGGATGTGCAGTTGTCCCAGGCTGGAGACCTCATCATGGAGGTTTACATAGAACAGCAGCTCCCGGACAA CTGTGTCACCCTAAAGGTATCCCCAACACTGACTGCTGAGGAGTTGACTAACCAGGTGCTGGAGATGCGA GGGGCGGCATCGGGGACAGACTTGTGGGTGACATTTGAAATTCTTGAACACGGAGAGCTTGAGAGGCCAC TGCACCCCAAAGAAAAGGTTCTAGAGCAGGCCTTGCAGTGGTGCCAGCTTCCAGAGCCCTGCTCAGCCTC TCTGCTTCTGAGAAAAGTCTCTATGGCACACGCTGGCTGCCTCTTCACAGGAGTCCGGCGTGAGAGCCCA CGAGTGGGGCTGCTACGGTGTCGTGAGGAGCCACCTCGATTGCTGGGAAACCGTTTCCAGGAGAGGTTCT TTTTGGTGCGTGGCCGATGCCTGCTGCTGCTCAAGGAGAAGAAGAGCTCTAAGCCAGAACGGGAGTGGTC TCTGGAAGGTGCCAAGGTCTACCTGGGAATCCGCAAGAAGTTAAAACCTCCAACACTGTGGGGTTTCACA TTAATATTGGAAAAGATGCACCTCTGTCTGTCCTGCATGGATGAGGAGGAGATGTGGGACTGGACCACAA GCATCCTTAAAGCCCAGCATGATGACCAACAGTCCGTGGTTTTACGTCGCCGTTCCTCCTCTGACCTTGC TCGTCAGAAGTTTGGCACTATGCCCTTGCTGCCCATCCGAGGGGATGACAGTGGAGCCACCCTTCTCTCT GCCAATCAGACCCTGCGGCGACTACACAACCGGAGGACCTTATCCATGTTCTTTCCAATGAAGTCACCCC AGGGATCTGTGGAAGAGCAAGATGAGCTGGAGGAGCCTGTGTATGAAGAGCCAGTATATGAGGAGGTCGG GGCCTTCCCTGAGCTTACCAAGGACACGACCTTCTCTTCCACATGGGAGTGGTCAGCCAAGTCAGATCCA TCCCTTACCAGCCAGAGGTCCTTTGATCAACCCCCCCTCTCCAAGGCAAGCATGTTGGGCCACGAAGAGA GGATACCTGACCCTCCTCCGGGCCCCCCTTCAAAAAGCAGTTCTCAGGCACGAGGGTCCCTAGAGGAACA GCTGCTTCAGGAACTCAACAACCTCATCCTGAGGAAAGGAGAGCCTGCCTCGTGCCCAGAAAGCTCTTCC CAGCCCACCAGTCCCCAAGCCCCAAGCCCCACCAGCCTTCCAACACCGACCCCCAGCTTACCCACCCAAC CGCCCTGCACTTCCAATCCACCCTCCAGCCAGCCTCTCACATGACCCCAGGGCCCGCAGTTGAGGAGGTA GGTAACCAGAGGCGAAGCCAGGCTCTCACCATGGCAGATTACTGCTGGGAGCTGCTTCTGCCCTGGATGG ATGACTCTGGAATCCAGTATGGTACCATGGAAGGAGCACTGGACTGAGAGCCCTGGAGACTGTGTGTCTC AGGGAAGATCATGGCCCCTCTCTGGGCCCAGCCCATTTGTCTGTACTATGAAGTCACTAAAAGAAGGAGA GGGGTGAACACCGAACTGTTTCTGAGCTAGGAGCCCTGATATTTCCCCTCAATAAGGGCAGCTCCTAGTT GATATATTTGATATAGGGGTTCAGTGAGAGCTTTGTGGCCTAAGAATGGTTTTACTACATTATAGGAAAG TGAAGGTTTGGAAGCTAGAGATGGGCATGATATCTAAATCTATGTTATTCCGCTTCCTATGTACTGGAAC TGCCTTGAGGCCCTAGAGTGGCTGATTGGGATTGGGAGCTGGGGCAAAGGGGAGTCGAAGAGGAATGGAT CACTATTAATAAAATTTGGAACCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAA
Notes:	The probe template was PCR amplified from IMAGE:6856630 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6856630 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12067 same embryo
	EMAGE:12071 same embryo
	EMAGE:12069 same embryo
	EMAGE:12068 same embryo
	EMAGE:12072 same embryo
	EurExpress:euxassay_008606 same experiment
	MGI:4823207 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source