EMAGE mouse gene expression spatial database

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EMAGE:12079

Slc24a5 solute carrier family 24, member 5 ( MGI:2677271)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_006990. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_006990_01	euxassay_006990_02	euxassay_006990_03	euxassay_006990_04

euxassay_006990_05	euxassay_006990_06	euxassay_006990_07	euxassay_006990_08	euxassay_006990_09

euxassay_006990_10	euxassay_006990_11	euxassay_006990_12	euxassay_006990_13	euxassay_006990_14

euxassay_006990_15	euxassay_006990_16	euxassay_006990_17	euxassay_006990_18	euxassay_006990_19

euxassay_006990_20	euxassay_006990_21	euxassay_006990_22	euxassay_006990_23	euxassay_006990_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12079_wholemount_strong.wlz

12079_wholemount_moderate.wlz

12079_wholemount_weak.wlz

12079_wholemount_possible.wlz

12079_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12079_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

mesenchyme

strong

spotted

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

diencephalon roof plate

moderate

regional

moderate expression: see section 14

choroid invagination

moderate

regional

moderate expression: see section 06 07 08 09 10 17 18 19 20

metencephalon part of 4th ventricle choroid plexus

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

inner ear

strong

spotted

strong expression: see section 01 02 03 04 05 06 07 08 09 10 16 17 18 19 20 21 22 23 24

pharyngo-tympanic tube

strong

spotted

strong expression: see section 01 02 03 04 21 22 23 24

liver

strong

spotted

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9462
Entity Detected:	Slc24a5, solute carrier family 24, member 5 ( MGI:2677271)
Sequence:	sense strand is shown >T9462 AGAGCACGCTGGAAATGCGGACAAAATGGGGCCCAACTTGGACAAGGAGGGTCCTGCTGCTGGGGATCTT TTGGGTCTCTGCATACCTGCCTGTCCGTGGTGTCTCCCTGCCCCCGCGCCTCCCAAGGGCCACAGGAAAC AGCACGCAATGTGCTGTCTCTCCAGCATCGGAGTTTCCCGAAGGGTTCTTCACCAAGCAGGAGAGCACGG ATGGAGGTATCGTAATCTACTTCCTCATTATCCTCTACATGTGCATGGCCATCTCCATCGTCTGCGACAA GTACTTCTTGCCCTCCCTGGAAATCATCAGTGACTCCCTTGGACTGTCGCAGGATGTTGCAGGTGCAACT TTCATGGCAGCTGGTAGCTCCGCCCCTGAACTAGTTACCGCCTTCTTGGGTGTATTTATCACAAAGGGAG ATATTGGTATCAGCACCATACTAGGATCTGCAATTTATAATCTCCTCGGCATCTGTGCTGCCTGCGGCCT CCTGTCCAACATGGTTTCAACACTCTCCTGCTGGCCCCTCTTCCGAGACTGTGCAGTGTATGCAGTTAGT GTAGGAGCAGTTTTTGGCATAATATTTGACAACCGAATTTACTGGTACGAAGGAGCAGGGCTGCTACTGA TATATGGGCTCTATGTTCTGCTGCTGTGTTTTGACACCACGATCAGCCGGCATGTCATGAAAACATGCAG TCCCTGTTGTCCCTGCCTTGCCAGAGCTATGGAGGAGAGAATTGAACAGCAGACACTGTTGGGATGGGAG GATGAGAGCCAGCTCTTCATTCGTCGCCAGTCAAGGACAGACAGTGGAATATTTCAGGAAGATTCCGGTT ACTCCCAACTTTCTCTAAGCTTGCATGGTCTTAGTCAGGTTTCTGAAGATCCACCAAGTGTTTTCAGCAT GCCAGAAGCAGACTTAAGAAGAATTTTTTGGGTTCTCTCTCTTCCTATTATTACATTACTTGCTCTGACA ACACCAGACTGCAGGAGAAAGTTTTGGAAAAATTACTTTGTGATAACCTTTTTCATGTCTGCACTATGGA TATCTGCATTCACATACATCCTAGTTTGGATGGTCACAGTAACAGGGGAAACACTAGGGATCCCAGACAC AGTGATGGGCCTTACTTTGTTGGCAGCAGGGACAAGCATACCAGATACAGTCACAAGTGTGTTAGTTGCA AGAAAAGGTAAAGGAGACATGGCTATATCTAACATCGTGGGATCCAATGTGTTTGATATGCTATGCCTAG GTCTTCCCTGGTTTATTAAGACTGCATTTACGAATGCATCTGCTCCTATAGAAGTGAATAGCAAGGGACT CACATATATAACAATCTCTCTCAACATTTCAATTTTATTTCTGTTCTTAGCAGTTCATTTTAATGGCTGG AAACTAGACCGAAAGTTGGGGGTGGTCTGCCTAGTGTTATACTTAGGACTTGCTACTTTATCAGTTCTAT ATGAAATTGGAATTATTGGAAATAATAGAATAAGGGGTTGTGGAGTTTGATATTTTTTAATAGTGTTATG TGGAAAGATGGAATGGTAGAGGGGCAAAAAGAAGAAAATTCATGTCTTCATTTAGGTCAAATAAAAAGCA TCTTCAACTTTAGAGTCTAACACTTAATTACTATTCTTTATAAGCAGAGAAAAGTAATTTAAACCAAACC AAAATCCATCCTAATTTGTCTGAGCCCTTTCTTTCCTTTAACTCACATACAAAGCAAAGATTTTAAAAGA AAACAAAGTATCTGTGAGCTTACACCAAGGTCGGGAAAACTGGAACAGACAAACTCTGCTACTGAAAATA ACAAAATGGCTTATTTCATTAAAAATGGTATGTCCATCCACTGAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30538386 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30538386 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12076 same embryo
	EMAGE:12075 same embryo
	EMAGE:12074 same embryo
	EMAGE:12077 same embryo
	EMAGE:12078 same embryo
	EurExpress:euxassay_006990 same experiment
	MGI:4828144 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source