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EMAGE:12092

Unkl unkempt-like (Drosophila) ( MGI:1921404)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:12092 EMAGE:12092 EMAGE:12092 EMAGE:12092 EMAGE:12092
"Pseudo-wholemount" of euxassay_008339. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_008339_01 euxassay_008339_02 euxassay_008339_03 euxassay_008339_04
EMAGE:12092 EMAGE:12092 EMAGE:12092 EMAGE:12092 EMAGE:12092
euxassay_008339_05 euxassay_008339_06 euxassay_008339_07 euxassay_008339_08 euxassay_008339_09
EMAGE:12092 EMAGE:12092 EMAGE:12092 EMAGE:12092 EMAGE:12092
euxassay_008339_10 euxassay_008339_11 euxassay_008339_12 euxassay_008339_13 euxassay_008339_14
EMAGE:12092 EMAGE:12092 EMAGE:12092 EMAGE:12092 EMAGE:12092
euxassay_008339_15 euxassay_008339_16 euxassay_008339_17 euxassay_008339_18 euxassay_008339_19
EMAGE:12092 EMAGE:12092 EMAGE:12092 EMAGE:12092
euxassay_008339_20 euxassay_008339_21 euxassay_008339_22 euxassay_008339_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:12092Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
12092_wholemount_strong.wlz
12092_wholemount_moderate.wlz
12092_wholemount_weak.wlz
12092_wholemount_possible.wlz
12092_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:12092_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
facial vii ganglion
weak weak
regionalweak expression: see section 21 22
glossopharyngeal ix ganglion
weak weak
regionalweak expression: see section 07 08 09 18 19
trigeminal v ganglion
weak weak
regionalweak expression: see section 04 05 06 07 08 09 18 19 20 21 22 23
dorsal root ganglion
weak weak
regionalweak expression: see section 07 08 09 10 11 12 16 17 18
liver left lobe
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12
liver right lobe
moderate moderate
regionalmoderate expression: see section 13 14 15 16 17 18 19 20 21 22 23
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T9436
Entity Detected:Unkl, unkempt-like (Drosophila) ( MGI:1921404)
Sequence:sense strand is shown

>T9436
CCGGCAACACTGTGGGGGCAGTCATAGGTTCTGCCTTAGATCTTCGTCTTAGTGACATAAACATCGCGTC
CTTAGATAAAGACTTAGAAGAACAGGACCTTGGATTGACAGGTCCCAGGTCGCTGGCAGGCTCAGCACCC
GTCACCATTCCGGGCTCCCTGCCCCGCTCGCCATCCTTGCACTCGTCCTCATCCCTATCCACCTCCCCAC
TCAGCTCGCTCTCCCAGTCCCTGTCGGGGCCACTCGTCTCCTCGGCCATGACGCCCCCCCAGCAGCCACC
ACCCCTCCGGTCGGAGCCGGCTACGCTGGGCTCTGCAGCCTCATCTTACAGCTCTTTAGGTCTGAACGGC
GTTCCCGGGAGCATCTGGGACTTTGTTTCTGGCAGCTTCTCTCCTAGCCCATCCCCCATTCTGAACTCCG
GCCCTTCGGCTTCCTCAAGTGCAAGTCCAAACAGTGCTGAGCTGGCACGGGTCAGGCGGCAGCTAGACGA
GGCCAAGAGGAAGATCAGGCAATGGGAAGAATCTTGGCAGCAAGTGAAGCAGGCCTGTGATGCCTGGCAG
AGAGAGGCTCAGGAGGCCAAGGAAAGAGCCCGTGTGGCAGACAGTGACCGGCAGCTGGCCCTGCAGAGGA
AAGAAGAGGTGGAAGCCAAGGTGAAGCAGCTGCAGGAAGAATTAGAGGGCCTGGGTTTGTCCTCACTGCC
AGGGCTTCAGAGCTTGGGTGACATCAGTGACATCCCTCTCCCCAAGCTGCATTCTCTCCAGAGCAAGCTG
CGCTTGGACTTGGAGGCTGTGGATGGGGTGATCTTCCAGCTCCGTGCCAAACAGTGTGTGGCCTGCCAGG
AGCGGGCCCATGGCACTGTCCTGCGGCCCTGCCAGCACCGTGTCTTATGCGAACCATGTGCGGCCAGTAC
CCCTGAGTGCCCCTACTGCAAGGGCCAGCCCCTACCATGGTGACCAGAGCCAGACCCATTCCTTCCCTGG
TGAGCAGTATAAAGGCCTGGGCATGCCACGTGCCACTACAGTGTCGAAACTTGCCTCTGGTACCCCACGC
CAGGATGTGAGCTCACACCCACTGTCCAACAGCCTTTCATATTATTTTCTTCCCTGCCATGTCCCACCAT
CGGTGTCAAGGTATTATCGAAGAACCTCTGAAGCACCACCAAACCCACTGCCTGTCCCCTAGGCAGGTAG
CATGCTTTTGGTGCCCCCAGTCCTGAGGCTGCTGTGGCCAGGCCAATGGGAGGTCGGGCCTCGTCAAAGC
ACTTTGTGAACCGAGGAATTAGATATTATAAATTGTATAAGCTGCTTTCTAGGTCCATGTTTTTTAATAT
GCGATAACATGAAGCTTTATAAGTATACTGTGAACTTGAAGTTTCCTATCAGTTTGTGGTTGACTCATAG
TAATGGTAACTACGTTAGATTTCATACTCTGAAACTATTTAGGAAAGGAACATTTATCTAGTAGTGAAGC
ACTAGAATTTTTACATTTTAAAATTAAGTGCAAACAAATAGTATTTATAAGCAGAGAGTGGTTATTGAAA
GACATATACATTTAGATGTTCTTAATTTTATGGAAGGAACTAATTTTCCTAAACATGTGCAGGTGCACAT
GTGTGTGGAGTTTTATATTCCAGTATCATAGAGTGATATCCAGAGCTCTGTCGCGCACTTATGTGATCCG
TGGCCACTTCCAAGTGCCTGTGCCTTGCAAGAGAAGTGAAGGAATTGGTTCATTTGGTTTTTTTCTTCTC
CCTTGAAAGACAATTTCATGCTAATGAAAAACACAGCAAAAGAGTCAGACATTCAGAGGAGTCCAAGATC
TACTGGAAGGAGCTGTGCTCTGATGTCTTATCGTCTGTAATGGCAACGGTGCTTTGTAACCTCTGAGTCG
CCTGGGGCACCCCAGCTTGTGCTGAACAAGGGGCTGCGAACCTGAGACTGCAGTCTGCTGCACCTGTGAC
CACCCTCACCTCCAGCTGATCCAGAGGGGCCCAGAGGCACGAGTCCAGGACAGTCTTGGCACACATCTGG
TACCTGAGCTGCACAGCCAGATAGTAATAGAATTCATCACAGGACCAGCAAACACAGCTCTGCTACAGTT
CACCCATCAGTTTCCAAGGTGAATGTGGCTGCTGCTCACAGCTTGGCCTCTGGTCTGTGGCTCAAATTCT
GCTGAGACTGTATCGAGGGTGGTGACTTCAGCTCTCTACCATAAAGACTGGGACTGTTCCTGGAAATCTA
GGGAGGAGACATCTGTCCAGGTGACCCCTCCCATGGTGGCAGACCTAGAACAGGGTAGGTATCCTACCTA
CAGGTTCTGCCCAGGCCTCGAAGTGGTTCTCCTTCCCCATGGCTTTGCCAAAGACTTTTAATAGCATTTT
TTAAGTGCAAAGTGACTAGATATAAAACATTTTTATCAGTGACCAAATTAGAATGTATTTAATATAGTAG
GAAATTTAAAAGCATTTTTTAATATAAGACAAACTGATAGAAAAGACCTATATCATTTTAAAGGAAATGG
ATCTTCAACATTCCACAGCTAGCCCACTACTTAATAAGGTAACTATTGACCTGATTTCAGTGACTTTTGT
TTGTAATTATAGTGATAATGATTTATTAGCTCAGTACCCCGATCATGTGTATTTTGTGCTACTGTTATCA
TGGTTTAAGATTGTTTATTTTTATTCATATTTATGAACTGTTTCCCATGTCAGGCTTTTGGTCTCTGTGG
TAACAGGACAGTGTTTGTGATTGATAGCTCATCGGGACAATTTCAAGTTGTATAGTTACATTAAGCTTTA
GATTGTATGTAAGCTACTCTGGTTAACTCCTCCTGGGCTGACATTCCAGGCTGGGGCTGGGCAGTTCTGG
GGCTGTTGTGATGGCTGGGGAGGCAGGCGGTAGTGCACCCTGGGTCCCATCCCATGCGGGGAGCTTTCCT
GAGCATCTGCTCTTCTGGAATCAGGCTGAGCTCTGAGGACAGGTGTGCAGGGGAGGACCAAAGAACGTCC
CAGAAGGGGCCACTTTTCCCATCCATTCCAGACCTCTTAGGAGCTGAGGCTTCTAGGATCTACCCTGGGT
GGGAAGGGCCAAGAAGGGGAGAGAGAGCCTTAAGGCCCGTGTGTGGGTAATGGAGGTGGGGCCATGGGTC
TAGGAACTGGGACCCACCACTGTCCTGATGCCTGTTGTCGTCTAACTCTCTAGTGTTGAATAGTAACAGT
ATATGGTAGTGTCAGAGTCTTGTGTACCTGGTTGGCAGGCCTTCCCCCAGTATGGATGCGCATGTGGATT
AACTTATGGAAGCCTTTGTGTTCATTTGTTCTCAGAAATTAGTTGAGAATGTTATGTGAATGGAATCTTC
TTAACCCAGATGGTCATATTTATAATACATAACCATTTACTTTAGTGAATTGTTAAAGTTGTTAACATTT
GTTTAATTTTTTTTACAATAGCATTTATGCAATGGTTTACAGAAGTCATGGAGTTATTTTTTATCAGTAT
GGAATTAATTAAAACTTTGAATCTTTAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:30619605 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30619605 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:12091 same embryo
 EMAGE:12088 same embryo
 EMAGE:12090 same embryo
 EMAGE:12089 same embryo
 EMAGE:12093 same embryo
 EurExpress:euxassay_008339 same experiment
 MGI:4829107 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS