EMAGE mouse gene expression spatial database

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EMAGE:12114

Dnase2a deoxyribonuclease II alpha ( MGI:1329019)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008427. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008427_01	euxassay_008427_02	euxassay_008427_03	euxassay_008427_04

euxassay_008427_05	euxassay_008427_06	euxassay_008427_07	euxassay_008427_08	euxassay_008427_09

euxassay_008427_10	euxassay_008427_11	euxassay_008427_12	euxassay_008427_13	euxassay_008427_14

euxassay_008427_15	euxassay_008427_16	euxassay_008427_17	euxassay_008427_18	euxassay_008427_19

euxassay_008427_20	euxassay_008427_21	euxassay_008427_22	euxassay_008427_23	euxassay_008427_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12114_wholemount_strong.wlz

12114_wholemount_moderate.wlz

12114_wholemount_weak.wlz

12114_wholemount_possible.wlz

12114_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12114_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vibrissa

strong

regional

strong expression: see section 05 06 07 08 09 12 20 21 22 23 moderate expression: see section 03 24 weak expression: see section 04

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 08 09 10 11 12 13 15 16 17 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9482
Entity Detected:	Dnase2a, deoxyribonuclease II alpha ( MGI:1329019)
Sequence:	sense strand is shown >T9482 TGAATCTCGTGACCCGGCTGTCCCCGGGTCTTTTAGAGTCCATTGCTGTCGCCTCATGGCAACACTGAGA TCGCTGCTGCTGGCTGCGCTGCTGTGGGTCCCTGCCGAAGCCCTGAGCTGCTATGGGGACTCCGGGCAGC CTGTGGATTGGTTCGTGGTATACAAGCTGCCGGCTCACAGCGGGTCTAGGGATACTCCAAAGGGACTGAC GTATAAATACATGGACCAGAACTCCGACGGTTGGCAAGACGGTGTAGGGTACATCAACAGCTCGGAGGGA GCCGTGGGCCGCAGCTTGCAGCCATTGTACCGAAAGAACTCCAGCCAGCTGGCCTTTCTACTCTACAACG ACCAACCTCCTAAATCCAGCTCAGCTCGGGACTCTACCGGCCATGGGCATACGAAGGGTGTCCTGCTCCT GGACCAAGAAGGGGGCTTCTGGCTGGTCCACAGTGTGCCTCGCTTCCCACCCCCTGCGTCCTCTGGTGCA TACACCTGGCCTCCTAATGCTCAAACCTTCGGCCAGACCCTACTCTGTGTGTCCCTCCCGTTCACTCAGT TTGCAAGGATTGGCAAGCAGCTAACCTACACCTATCCCCTTGTCTATGACCACAAGCTGGAAGGCTTCTT CGCTCAGAAATTACCTGACCTAGAGACGGTGATCAAGAACCAACATGTCCTCCATGAGCCCTGGAATAGC AGTGTAATACTCACTTCCCAAGCTGGGGCCACCTTCCAGAGCTTTGCCAAATTTGGAAAATTTGGAGATG ACCTGTACTCCGGATGGTTGGCAGAAGCCCTTGGCACCAACCTACAGGTCCAGTTCTGGCAAAATTCTCC AGGCATCCTGCCCTCCAACTGCTCTGGAGCCTATCAGGTTCTGGATGTGACACAGACAGGATTCCCTGGC CCATCTAGACTAACTTTCAGTGCCACAGAGGACCACTCCAAATGGTGTGTGGCCCCTCAAGGGCCCTGGG CCTGTGTGGGTGACATGAATAGGAACAAAGCAGAGACACACCGAGGTGGCGGCACAGTATGCACCCAACT GCCTTCCTTTTGGAAGGCCTTCCAGTCCCTGGTGAAAGACTGGAAACCCTGTATAGAGGGGAGCTGACTG AAGCCCATCGGAGCAAAGGACTAAGACTCCGCAGTCTAACCAGGTGGGGGCCGGACTAGCCTTTACCCCA GCACTTGGGAGGCAGAGGCAGGTGGATCGATTCTCTCTCTCTCTCTGGTTTTTCGAGACAGGGTTTCTCT GTGTAGCCTTGGCTGTCCTGGTCTACAGAGAGTTCCAGGAGAGCCAGGGCAACACAGAGAACCCTTGTCT CAAAGAAAAGAAGAAGAAGAAAAAAAAAGAAAAACAAGAAAAAGAGAAAAGAAAGAAAGAAAGAAAAGAC TCAGAGACTAGTTTTGACTTGAATTTGTTTGTGTGTCTGTTGGTGGGTTAAACCCAGGGCTTACCACATG GGACACAAGTACAGTGCTGCTGAGCCACACTCCCTGTCCCAGCTATGAATCTGAATACCAGTTCAGTCCC TTCCTAACTGGAGACCTTAACAGTGCCTTAACCTCTAGCTGTCTGTTGCAAAATGGGCATCACAGCATGT CTCTAAGAGGTTGTTAATACAACCAACTCTGTGCATGGCACTGGTAACCTTTTTCTTAAGATGGAGCCCA AGACACAGACCAGGGGTGGGACACCTTTTCCTTACAAAAGCTGTTACCACGTGTGGAATTGGCAGTGGAT GGGACATAGGAAGGCCCTAATGAAAGCCACCTTCTTACTTTCTGAGAAGCCAAGGGCCATTCCTGTCCAG AGAGGAGGTGTGAACCCCAAGAATCTCAGGCAGCATGGGCACTTTATTTATATGTGTACATATGTATACA GGCATCTGTACAGCACAGCACCGAAAGCCTGCTCCGCTATGGACCTAGAGATGCAGGGCTAGGTGCCCCA GTTTTGGTGAGGCCTGCAGGAGTCACAGCTTCAGCAGGACACCGGGAGTTCAACCCTGCTTCAGGCTTGG TGCCTGGGACCATGAGGGAAGCAGGTGGGACTGAAGGAGCTGCACTGCGGGGTACATTCTTTGTTGGGAC TAGGGTTGGAGTCTGGGGGTCAGGAGAGAGTTTGGGGGAGGCGGGCTTAGAGCGAAGTCCGCTTAGCGTG GTCCGCTCTTCCACCTCCTCTAGCACCCAGCGCTCCCTGCCCCTTGGGGCATCAGGCATCAGAGGCATCT GGGCTACAGGTTCCTTCAATCCCTTGGAGTCTGTGCCCAGAGGCTGGGGTGCCTGGGTCTTGACCCCTGG CCGAGCAGGCTCTACAACAATGGGCACCGGGGGTGTGCCTGCCTCACCAGAGCTACTGCGCCGGCCTGTC TCATGTTCCTGCACTGGACTTAGCGCTGCCTTGGCCAGGGCCCTGGCCACCCCACCTGGGCCATCCTCAG TCTGCACGCTCTGGTGCCTTGTGCAACCCGAGTCACGCTCCAGGGTCCGGCTCCCAGGGGTTGTCGCGCG AGGTGGGGTGCAAGCCTCTGTACCTCCCGCGGAATCCTCCTTGCTTGACGCCATAGGTCTCTGGACACCA TCGGGTAGCAACGGGTCCGCGCCCAAGCTTAGGGGTGATTCCTGGCGACCCAGGCGTCGCACGGCGACCT GCCGGGTCGCCCCAGGACCCTCAATAGGAGGCGTCTCACCATCAGACTCAGCGAGGCTATGCAGGGCCAG CTCACCATGGAAGTCCTTTCGGAAATCAGGGTGTCCCACTTCCAGGCTGTGTTTGCGCAGTGCGCCCTTC TTGTCTGCTCCCAGCGAAGCCCCTAGTTTCTCCGCTGACTGCACGCGCTTGAGCAACGGCGAGCGTGGTG GCTCTGCACTTTTGGGGCGCGGGCGCACGACAGGTGGCGATGAGTGTAGTTTGGCAGGGAAGCTCTGCGT GGTATGTGAGCTGCCCACCGTGTGGCCTGGCAGCGGCGGCGGCGACGCCTGCGTCGGGGATGGTGTGTGC GCTAGAGGTGACAGCGGGATGTTGCCAGCCGATTTACAGCGCGCTGAGCGGTACTGGCGGTGTAGTTTCG GTGACAGCCCGTGCAAGGTGCTGGGCCTGATGTGGTGCGAGGCGGATGAGGCGGGGGAATTAGGCGTGCT GGAAGCTGGCGAGCTACTCTGTGACGAGGCGCCTGCAGGCCAGAGAAGGTGCAAACATTCTGAGCACCTC AGTGTCCTAGTCCCCTACCCCGCGCAGGCGCAGAACATACCTAGGTAGGCGGAGTCAGGTGTAGAGCGAT AGCTATGTGTGGGCGAGCGTGCGGGTAGTCCGTGCGTGGGCGAGCCCGGAAGACTGTCGCTGGACGACAA GGACCGGTTCAAAGAGGACAGCGATCGACTGGTGTGCAGCAGGTTCGACTGCTTTGTGATCTTCCGAAAG AGAGAGCTCCGCTTCTTGCTGGAGGACAAATACCAAAAGGATTAAAGGCATGCGGCCCTTTGTCTTTGGG GCTAGAGAGATGACTCAGCAGTTAAGAACCGTGGCTGCTCTTCCAGAGGACCAGACTGGCTTTTCAGCAG TATGTGGCATCTAACAACCACCTGTAACTCTAGTTCCAAAGAATCTGGCGCCCTCTTCTGGCCTCTGCAG GCATCAAGCACACATGGAGTACACATAAACAGAAGCAATCAAAATGCTAATACAAATTTAAAACAATAAA AACTAAAACAAAACTGGTCTGTTGGGGCTGGAGCCATGGACAGTTGGTTAAGAGGGTATATTGCTCTTGA TCGCCTCTGTTAGACAGCTAACTCACAATGCCTGTAACTCTAGCATCTAGGCAATCCAACCAATACCCTC TTCTGGCCTTCAGAGGCACATCCACAATCAGACACAAACCTATACATAATTAAAATGTTTTAAAAATTAA AAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30356047 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30356047 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12112 same embryo
	EMAGE:12113 same embryo
	EurExpress:euxassay_008427 same experiment
	MGI:4824360 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source