EMAGE mouse gene expression spatial database

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EMAGE:12136

Pan3 PAN3 polyA specific ribonuclease subunit homolog (S. cerevisiae) ( MGI:1919837)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008398. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008398_01	euxassay_008398_02	euxassay_008398_03	euxassay_008398_04

euxassay_008398_05	euxassay_008398_06	euxassay_008398_07	euxassay_008398_08	euxassay_008398_09

euxassay_008398_10	euxassay_008398_11	euxassay_008398_12	euxassay_008398_13	euxassay_008398_14

euxassay_008398_15	euxassay_008398_16	euxassay_008398_17	euxassay_008398_18	euxassay_008398_19

euxassay_008398_20	euxassay_008398_21	euxassay_008398_22	euxassay_008398_23	euxassay_008398_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12136_wholemount_strong.wlz

12136_wholemount_moderate.wlz

12136_wholemount_weak.wlz

12136_wholemount_possible.wlz

12136_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12136_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9275
Entity Detected:	Pan3, PAN3 polyA specific ribonuclease subunit homolog (S. cerevisiae) ( MGI:1919837)
Sequence:	sense strand is shown >T9275 ATGACAGTGCCAAGCCATACACAGGCCACGATCTTCTGACTTCATCTGCTTCATCCTTGTTTAATGACTT CGGTGCCCTCAACATCTCCCAGAGAAGGAAGACACCAAATCCTACTGCAAGCGAATTTATACCTAAAGGA GGATCAACCTCCAGGCTGAGTAACGTGTCCCAGTCAAATATGTCTGCCTTCTCTCAAGTTTTCTCTCACC CATCCATGGGAAGTCCTGCTACTGCTGGATTAGCACCAGGGAACAAGCTACCCTCTGAGTCAGCACTGGA CTTGAAGATAAATCCTACAGTTCATGAAACAGACTGATTCGACTAAAGGATGGATAGTTTGGGCAGCGTT GGACAGCTCTATGAGGGGAATGTCACTGTCTGCTGGATCTTCCCCTCTCCATTCCCCCAAAATTACTCCA CACACCTCTCCTGCTCCTAGAAGAAGAAGCCACACTCCAAACCCAGCAAGTTTCATGGTGCCGCCTAGTG CCTCCACTCCTGCCAATAACCCTGCTCCTCAGCCTCCGTCCTCTGGTCAGGTGATCCAGAAGGAGACTGT CGGGGGGACGACTTACTTCTATACAGATACAACCCCAGCACCTTTGACTGGAATGGTATTTCCAAACTAT CATATTTATCCTCCAACCGCACCTCATGTTGCTTATATGCAACCAAAAGCAAATGCACCTTCCTTCTTCA TGGCCGACGAGCTCCGACAGGAGCTGATCAACAGACATTTAATAACGATGGCTCAAATTGACCAAGCAGA TATGCCAGCAGTCCCCACAGAAGTTGACAGCTACCATAGCCTGTTCCCTCTAGAACCACTGCCACCTCCC AACCGGATACAGAAATCAAGTAATTTTGGATATATCACGTCTTGCTACAAAGCTGTAAATAGCAAAGATG ATCTGCCATATTGCCTTCGGAGGATACATGGTTTTCGTCTTGTTAACACAAAGTGCATGGTGTTGGTTGA TATGTGGAAAAAAATTCAGCACTCAAATATTGTAACTCTGCGTGAAGTGTTTACCACTAAAGCATTTGCC GAGCCTTCTCTGGTGTTCGCCTATGATTTCCATGCTGGAGGAGAAACTATGATGAGCAGACACTTTAACG ACCCGAATTCTGATGCCTACTTCACAAAGAGGAAGTGGGGCCAGCACGATGGACCACTGCCCAGGCAGCA TGCTGGATTGTTACCAGAGTCTCTCATCTGGGCATACATTGTACAGCTGAGTTCTGCTCTGCGGACCATT CATACAGCAGGTTTGGCCTGTAGAGTAATGGACCCAACGAAGATTCTGATAACCAGCAAAACAAGGTTAC GAGTAAATTGTGTAGGAGTCTTTGATGTTTTAACATTTGATAACAGTCAAAATAATAATCCTTTGGCATT AATGGCTCAGTACCAGCAAGCAGATCTGATATCATTAGGAAAAGTTGTGTTGGCTTTGGCTTGCAACTCT TTGGCAGGAATTCAGCGAGAAAATTTACAGAAAGCCATGGAATTGGTGACAATCAACTACTCCTCTGACC TGAAGAACCTCATCTTGTACTTGTTGACTGACCAAAACAGGATGCGGAGTGTGAATGACATCATGCCCAT GATTGGTGCTCGGTTTTACACTCAATTGGATGCTGCTCAAATGAGAAATGATGTTATCGAGGAAGACCTT GCTAAGGAGGTTCAAAATGGAAGACTGTTTAGGCTTCTAGCAAAATTGGGAACAATCAACGAGAGGCCGG AGTTTCAGAAGGATCCAACCTGGTCAGAGACTGGAGACAGATACCTGTTGAAACTCTTTAGAGATCATCT TTTTCATCAGGTGACAGAAGCAGGTGCTCCCTGGATCGACCTCAGTCACATCATTTCTTGTCTTAACAAG CTAGATGCTGGTGTGCCAGAAAAAATCAGCCTGATTTCCAGAGACGAGAAGAGCGTGCTGGTGGTGACAT ACAGTGACCTAAAACGCTGCTTTGAAAATACCTTTCAAGAACTGATTGCGGCTGCAAATGGTAACGATCG GAACAGTAACTGAAGGAGCAGGCGCTGCTCTTCTCCAGGTTCATCTAGAGGCTTCTTCAAAGAGGCGTCT GTCTGCCAGTCACATACACTTATCTTGACGTAATTTTTAATAGCAAAATGAAATTGTGCTGAAGGCCTAA AAGTGGAGGAGTTATATTACCTTAGGGTCTTTCCAGTTTATTTGTTTTTTGTTTTTTTTTTTGTTTTTGT TTTTTGTTTTTTGTCTTTGAACTACAGCTTTATAATGGTACTTTACACTGGATAGACTCAGACACTGTCG AGGAGGTGAGCTGAGCACAGCAGTGGCCTGATGCTTTGCGGCCTGACTCTGGAGGGACACTTTTCTGCTG TGCCTTTGCACTCACTGCTTAACCCAAAGGAACAGGACTTTTGATAAAACTCACCCTGTAGGGTCGGTCA TCTGAGAAGGCCATTTCAAAAGTTGGAGTTTTCCTCCATGGAACTTACTATTTGGACATGCAGTTGTCGC CAAACTCAAGTATTGTGGGATTTGTGGTCACTTCGATACTGAAGACTGCCAAATACATTGGGATTTTCTC TTAAACAGCCGCCATGAAGACTTCATCTCCTTCCCGGTGCTGTGTGTTGAGCGCGCACTGGGTGTGCACC CCATACCTGGGAAGAAATGTGGGGATTTGGAGGTCAGGACAGACTCCAAGCACTTGCAACTGATGTTACT GTCTTCATTTTTAACAAGTATGCGTATTTGTATATTTCAGAGAAGTTTTTAACATTTCTCTGTCCACTTT TTATAAGCTTTAAAATGATTTTTCTCTGCCTTGAAATTTGCATCAAGAAAAAGCACCTCTCTTCACTGAA AGCTTTGAGGACTAGAGACACACTTCCCATGTCTAACAAGCACGTCTGTATCTGAGAGCGCTAGCATCAG TTGTTAATGAGAAAATCAATTACTGCATTTGATCTGGATGGATTTTAGAAGAACATAATGTTCAATATTC CAAGGATAATGAACATTGCCACCATGATGTTCCTTGTGTTTAAAGGGAACGAGAACTTTCGGTGTACCAC GCAGAAGTCTTTATCATTAGCTCATGTATTTGAGGAAGAGCAGCTGTCTTTTTATATGTTTTTTGACAAA TCATATTGTAATTCTTTTGTACAAAAAAGAACTACTTGTATTCTAGAAGAAATATGAAATGCTTAATTTA TAAGCGGGCTGGAGATTTTTTTCCAATATTGTTTTCTTTGAAAATGAAAGGGGATCATCTATTTTAGTTT TGGGGGCCTGGGAACTTTTTGAAAATTTTCATTTGTGGACCAATGTTATGTGAATGCTAAGGAAGGGCAG GGGTAACATAGGGCTTGGACCTCTAGTCCTGCATAGACCAGCAAACTCCCCTCTGCAAGGCAGGTTCACA TCACAACCCCGAGTGTCTGCATCAGAAATGCTAGTCTGCTTCCGCCCCAGCAACCTCAGGACTTGGGTTG GGTATAAAAGGTAGTCAGCTGAAAGTACCTAAGTAAATAGTGTCAGCCAGAAAACAGTCGGTGTCAGGTG ATACGTATTTTTTAGCTTTGTTTTATATTTATTTTTCATTTCATTTTCATTGGGAATGGTTTTCAAAAGA ACTCTTGAGTTCTGCTTTGATGGGTTTCTTTTTTTTTTTTTTCTGTTTTGTTTTGCTTTGCTTTTTTTTT TTTTTTTTTTTTTTCTGGGAGCCCTCTTTTCCACAGTGTAAACCCATATAGAAGGTTGTCTGAAAGTTAC TATTTAGTGCATAGGAAGAGTTTAATATCTGAGAGTATCGAATGAAGGATGTGAACTCATCATAACTTCC TGTTATGACAGACACAACACGGAAAGGACAACCCTTGAAATCATGTCGCGTCGCTCACTTCAGCAGTGTA CCCATCTCCAGTCCTGTTAGTGTATTCACTTCACTGCAGATACCTTTGAGGGTGCCCTTGTGTTCTGCTT TGACCTTGGTTCTGTCATTGGATAGCAACATTGTCTCTAGAAAGCACCAGTACGTTTAAGTTCTAATGTC ACAATCCCAGTACCTGTTGCTCATCTTTAACCCTGTTTTCAGTCTCTACGTGTATAGCAGTATTTTTTAA TAAAGAATTGCAGAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6838444 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6838444 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12139 same embryo
	EMAGE:12137 same embryo
	EMAGE:12135 same embryo
	EMAGE:12140 same embryo
	EMAGE:12138 same embryo
	EurExpress:euxassay_008398 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source