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EMAGE:12160

Ghsr growth hormone secretagogue receptor ( MGI:2441906)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:12160 EMAGE:12160 EMAGE:12160 EMAGE:12160 EMAGE:12160
"Pseudo-wholemount" of euxassay_008371. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_008371_01 euxassay_008371_02 euxassay_008371_03 euxassay_008371_04
EMAGE:12160 EMAGE:12160 EMAGE:12160 EMAGE:12160 EMAGE:12160
euxassay_008371_05 euxassay_008371_06 euxassay_008371_07 euxassay_008371_08 euxassay_008371_09
EMAGE:12160 EMAGE:12160 EMAGE:12160 EMAGE:12160 EMAGE:12160
euxassay_008371_10 euxassay_008371_11 euxassay_008371_12 euxassay_008371_13 euxassay_008371_14
EMAGE:12160 EMAGE:12160 EMAGE:12160 EMAGE:12160 EMAGE:12160
euxassay_008371_15 euxassay_008371_16 euxassay_008371_17 euxassay_008371_18 euxassay_008371_19
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euxassay_008371_20 euxassay_008371_21 euxassay_008371_22 euxassay_008371_23 euxassay_008371_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:12160Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
12160_wholemount_strong.wlz
12160_wholemount_moderate.wlz
12160_wholemount_weak.wlz
12160_wholemount_possible.wlz
12160_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:12160_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
ventral grey horn
moderate moderate
single cellmoderate expression: see section 10 11 12 13 15 16 weak expression: see section 14
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T35172
Entity Detected:Ghsr, growth hormone secretagogue receptor ( MGI:2441906)
Sequence:sense strand is shown

>T35172
GCTGAAGCGATAGATTGCCTATTTACGTTGGAAACATCAAAAGCCAACCACAACTAGAAGCACTTTTAAA
GAAATATTTTTCAAATCCATTTGAACACAACAGTCACTCTAAATGGCCAACGTTAACCAGCTGACTCTTC
CGCTAGTTCTTGGGAGTGCGTTAGCAGGCACACTGATGCACTTGGTTCTGTGAGATACGTGCTAGGCATC
CAGAGCCTAGCTCCTCATTCGTGCTCAGTAAATTAAAAGGAGTCATTTTGGTTTCCTGGGGCTTCAGAGT
TGTGTCTATAAATACTTTACACTTGAACAGAAACACATTTAATTAGTTTCTTAATCTCTTCACTTAGCCC
CATCTCTTGGGGTCCACACCAGAAGTCCTGAACTTACATCCTGAGACTGAATTACAGAGAAAGGGCCACC
CTATTTCCCACTGGCTAACCTGAAAAGTGAAAGAATGTGTTTAGAGAAAGGAATCTAATTTGTCCTCTTT
ACTTATTATCTTTTAAAAAATTGCTTACAAGTTCTTTATGTTATTACATTTCTTGTTTTGTTTAAGACTC
AGGGCAGAAGAAAATTACACTGACACCAATTGTTTGGGAAAGCTCCCCTTTGGTCAACATTTTGGATGTG
CCTGCTATGTTCTGAGCTACAAGAAATTAACAAGCCAAAGATTTTATTTATTCTTAGTGATCTCACTATC
ACCTCTGGGTCTTGTTGCTGTGGAAAATGGATAAAGATGTTTGAGTTATGGAGTAGACATTTCTAGTGGG
CACTCTATCAGATTTCCCCACCACAGGACTACAATGAGCCATTCAGCCCACTCGTGTGTTGTATACTTGG
CTGCAAATATATTTGAATAGGCTGTCACTACAATCTTTGTAGACAGAGCTAATATGTCTGCTAGTGAGAA
TCACACTGAAGGCATTCAGAAAGGAAAGAAGCCAGCTTC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 69997. Forward Primer - name:069997_F_cDNA_Ghsr, sequence:GCTGAAGCGATAGATTGCCTAT; Reverse Primer - name:069997_N_SP6_cDNA_Ghsr, sequence:GAAGCTGGCTTCTTTCCTTTC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:12158 same embryo
 EMAGE:12161 same embryo
 EMAGE:12157 same embryo
 EMAGE:12156 same embryo
 EMAGE:12159 same embryo
 EurExpress:euxassay_008371 same experiment
 MGI:4825052 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS