Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:12175

Gpr132 G protein-coupled receptor 132 ( MGI:1890220)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:12175 EMAGE:12175 EMAGE:12175 EMAGE:12175 EMAGE:12175
"Pseudo-wholemount" of euxassay_008312. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_008312_01 euxassay_008312_02 euxassay_008312_03 euxassay_008312_04
EMAGE:12175 EMAGE:12175 EMAGE:12175 EMAGE:12175 EMAGE:12175
euxassay_008312_05 euxassay_008312_06 euxassay_008312_07 euxassay_008312_08 euxassay_008312_09
EMAGE:12175 EMAGE:12175 EMAGE:12175 EMAGE:12175 EMAGE:12175
euxassay_008312_10 euxassay_008312_11 euxassay_008312_12 euxassay_008312_13 euxassay_008312_14
EMAGE:12175 EMAGE:12175 EMAGE:12175 EMAGE:12175 EMAGE:12175
euxassay_008312_15 euxassay_008312_16 euxassay_008312_17 euxassay_008312_18 euxassay_008312_19
EMAGE:12175 EMAGE:12175
euxassay_008312_20 euxassay_008312_21

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:12175Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
12175_wholemount_strong.wlz
12175_wholemount_moderate.wlz
12175_wholemount_weak.wlz
12175_wholemount_possible.wlz
12175_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:12175_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
thymus primordium
moderate moderate
regionalmoderate expression: see section 10 11 12 13
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T35190
Entity Detected:Gpr132, G protein-coupled receptor 132 ( MGI:1890220)
Sequence:sense strand is shown

>T35190
GTGTTTGACATGAAGGTGGAGAAGAGTTTCTGCTTTGAGCCCCTGAGGATGAACAGCAAGATAGCCGGCT
ACCACTACCTGCGTTTCACCTTTGGCTTTGCCATCCCTCTCGGCATCCTGGCGTTCACCAATCACCAGAT
CTTCCGGAGCATCAAACTCAGTGACAGCCTGAGCGCTGCGCAGAAGAACAAGGTGAAGCGCTCCGCCATC
GCGGTCGTCACCATCTTCCTGGTCTGCTTTGCTCCCTACCACGTGGTACTCCTCGTCAAAGCTGCCAGCT
TTTCCTTCTACCAAGGAGACATGGATGCCGTGTGTGCCTTTGAAAGCAGACTGTACACAGTCTCTATGGT
GTTTCTGTGCCTGTCTACAGTCAACAGTGTGGCTGACCCCATCATCTACGTGCTGGGTACAGACCACTCT
CGGCAAGAAGTGTCCAGAATCCACACAGGGTGGAAAAAGTGGTCCACAAAGACATATGTTACATGCTCAA
AGGACTCTGAGGAGACACACTTGCCCACAGAGCTTTCAAACACATACACCTTCCCCAATCCCGCGCACCC
TCCAGGATCACAGCCAGCGAAGCTAGGTTTACTGTGCTCGCCAGAGAGACTGCCTGAGGAGCTCTGCTAA
GTCCACTCTTCCTCAAAACTAGCACCAGTCACACATACCTGGTCCTCTGAGTCACCGTCTGGGGTGTCCA
CAGCACTATAGATGCCTTT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 95484. Forward Primer - name:095484_F_cDNA_Gpr132, sequence:GTGTTTGACATGAAGGTGGAGA; Reverse Primer - name:095484_N_SP6_cDNA_Gpr132, sequence:AAAGGCATCTATAGTGCTGTGG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:12173 same embryo
 EMAGE:12176 same embryo
 EMAGE:12177 same embryo
 EMAGE:12174 same embryo
 EurExpress:euxassay_008312 same experiment
 MGI:4825172 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS