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EMAGE:12189

Timm22 translocase of inner mitochondrial membrane 22 homolog (yeast) ( MGI:1929742)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:12189 EMAGE:12189 EMAGE:12189 EMAGE:12189 EMAGE:12189
"Pseudo-wholemount" of euxassay_007063. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_007063_01 euxassay_007063_02 euxassay_007063_03 euxassay_007063_04
EMAGE:12189 EMAGE:12189 EMAGE:12189 EMAGE:12189 EMAGE:12189
euxassay_007063_05 euxassay_007063_06 euxassay_007063_07 euxassay_007063_08 euxassay_007063_09
EMAGE:12189 EMAGE:12189 EMAGE:12189 EMAGE:12189 EMAGE:12189
euxassay_007063_10 euxassay_007063_11 euxassay_007063_12 euxassay_007063_13 euxassay_007063_14
EMAGE:12189 EMAGE:12189 EMAGE:12189 EMAGE:12189 EMAGE:12189
euxassay_007063_15 euxassay_007063_16 euxassay_007063_17 euxassay_007063_18 euxassay_007063_19
EMAGE:12189 EMAGE:12189 EMAGE:12189 EMAGE:12189 EMAGE:12189
euxassay_007063_20 euxassay_007063_21 euxassay_007063_22 euxassay_007063_23 euxassay_007063_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:12189Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
12189_wholemount_strong.wlz
12189_wholemount_moderate.wlz
12189_wholemount_weak.wlz
12189_wholemount_possible.wlz
12189_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:12189_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T8419
Entity Detected:Timm22, translocase of inner mitochondrial membrane 22 homolog (yeast) ( MGI:1929742)
Sequence:sense strand is shown

>T8419
TGAAAGGTACTAGAGCCCCTACGGTCATGGCGGCTACCGCCCCCAAGGCCGGGGGTTCAGCTCCTGAGGC
AGCGGGTTCTGCCGAAGCTCCGTTGCAGTACAGCCTTCTTCTTCAGTACCTGGTGGGCGACAAGCGTCAG
CCCCGGCTTCTGGAGCCTGGCAGCCTGGGCGGGATCCCGAGTCCTGCCAAGAGCGAGGAGCAGAAGATGA
TCGAGAGGGCAATGGAAAGTTGCGCCTTCAAGGCTGTGCTGGCCTGTGTAGGAGGGTTTGTCTTGGGAGG
CGCGTTTGGTATCTTTACTGCTGGCATTGATACCAACGTAGGCTTTGACCCAAAGGACCCTTACCGGACA
CCAACTGCAAAAGAAGTCCTGAAAGACATGGGACAGAGAGGAATGTCCTATGCCAAAAACTTTGCCATTG
TGGGCGCCATGTTTTCATGTACTGAGTGTCTGGTAGAGTCTTACCGGGGAAAGTCGGACTGGAAGAACAG
CGTCATCAGTGGCTGCATCACTGGCGGAGCCATCGGCTTCCGAGCTGGAGTAAAGGCCGGGGCCATAGGT
TGTGGAGGGTTTGCTGCTTTCTCTGCTGCAATCGATTATTACCTACGGTGAAAGAAACAGCCGGGAAGGA
AAGAGGACGCCAGCCACCTTCAGAGCTGCTCTGTGGAGCAGTTTCTACACACTTGGACTGCTTCAGGGGT
GAAGCCAAGTGCATCCCTCTACAGTTGATGGTGTGAAGGACACACCTCGCTGCTCGATCGCTCTCTGCAG
CCATTGGAACAACCATACTTTTCCACTTGAATTCCAACCAGGTTTTTCAGGGGAGGTGGTATGGCAGAGT
GTCTCCTTGCAGTAAACAGATCAGGAGGCAGTGTGTGGACTGTTACGCATTAAGCTTTGAACCAGGCTCA
GTTCTGACCTGGCTCAGCTCCCCAGCTTTGAATAGGGAAGTGGCAGTGTTTGCCCATAGCCGGGAAGCTC
TTCTTCCTTCTGTGTGCAAGTATCCATCATGAGTCCCAGGGCAGGGCAAAGTAAACTTATGCCATCGAGG
CAATTATGTGATTTCAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:5688754 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5688754 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:12190 same embryo
 EurExpress:euxassay_007063 same experiment
 EMAGE:29719 same embryo
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS