EMAGE mouse gene expression spatial database

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EMAGE:12215

Wap whey acidic protein ( MGI:98943)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008338. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008338_01	euxassay_008338_02	euxassay_008338_03	euxassay_008338_04

euxassay_008338_05	euxassay_008338_06	euxassay_008338_07	euxassay_008338_08	euxassay_008338_09

euxassay_008338_10	euxassay_008338_11	euxassay_008338_12	euxassay_008338_13	euxassay_008338_14

euxassay_008338_15	euxassay_008338_16	euxassay_008338_17	euxassay_008338_18	euxassay_008338_19

euxassay_008338_20	euxassay_008338_21	euxassay_008338_22	euxassay_008338_23	euxassay_008338_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12215_wholemount_strong.wlz

12215_wholemount_moderate.wlz

12215_wholemount_weak.wlz

12215_wholemount_possible.wlz

12215_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12215_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7213
Entity Detected:	Wap, whey acidic protein ( MGI:98943)
Sequence:	sense strand is shown >T7213 GACACGAGAAGCCCAGCGCCCTCCTGAAAGGAAGGACTGCCTGCTGCCACTCTGAGACAGTGGTCTGAAG ATCCCAGAGGAGGAATTTGGCCAGGTGGCCGATGACAGCCCAACCAAGGAAAGGTGTCTCAGGACAACAC CACCAGCAGTGCCCAGGCCCCGGGTACCATGCGTTGCCTCATCAGCCTTGTTCTTGGCCTGCTGGCCCTG GAGGTGGCCCTCGCTCAGAACCTAGAGGAACAAGTCTTCAACTCAGTTCAGTCCATGTTCCCAAAAGCCA GCCCCATTGAGGGCACAGAGTGTATCATCTGCCAAACCAACGAGGAGTGTGCCCAGAATGCCATGTGCTG TCCCGGTTCCTGTGGTAGGACCCGCAAAACTCCTGTCAACATTGGTGTTCCGAAAGCTGGCTTCTGCCCT TGGAATCTACTCCAAATGATCAGTAGTACCGGGCCGTGCCCAATGAAGATAGAATGCTCCAGCGACCGTG AGTGTTCTGGCAACATGAAATGCTGCAACGTCGACTGTGTCCTGACATGTACACCCCCAGTGCCAGTGAT AACCCTTCAGTGAGCAGCCGGCCCTGGCATCCCTGCCTGTCGGGAGTGACCAGCCCAAGTCTATACAGCA AGACCCTTCACTCCTGGATCCAGAGAGAACATAATGCTTTCAATCTGCTGCTAATAAAAATCCATTTGGC TTTAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4039857 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4039857 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12217 same embryo
	EMAGE:12216 same embryo
	EMAGE:12219 same embryo
	EMAGE:12218 same embryo
	EMAGE:12220 same embryo
	EurExpress:euxassay_008338 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source