EMAGE mouse gene expression spatial database

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EMAGE:12240

Paip2b poly(A) binding protein interacting protein 2B ( MGI:2386865)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007045. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007045_01	euxassay_007045_02	euxassay_007045_03	euxassay_007045_04

euxassay_007045_05	euxassay_007045_06	euxassay_007045_07	euxassay_007045_08	euxassay_007045_09

euxassay_007045_10	euxassay_007045_11	euxassay_007045_12	euxassay_007045_13	euxassay_007045_14

euxassay_007045_15	euxassay_007045_16	euxassay_007045_17	euxassay_007045_18	euxassay_007045_19

euxassay_007045_20	euxassay_007045_21	euxassay_007045_22	euxassay_007045_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12240_wholemount_strong.wlz

12240_wholemount_moderate.wlz

12240_wholemount_weak.wlz

12240_wholemount_possible.wlz

12240_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12240_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4326
Entity Detected:	Paip2b, poly(A) binding protein interacting protein 2B ( MGI:2386865)
Sequence:	sense strand is shown >T4326 ACCTCGGGGCGGCGTTGTCCGCGCGGCCAGGCACCTGGAAGGGTGAAGGCGAGAGGCAGATGACGACGCT GAACACAGGCTCCGCAAGAATCTCCATAATGAATGGATCCAGTGTGGCAAGTACATCACCCAGTGTGAAA TGCAAAGAAGACCAAGGATTAAATGGACATGAGGAAAAGGAAAACCCATTTGCAGAATACATGTGGATGG AAAATGAAGAGGATTTCAACAGACAGGTGGAGGAAGAGCTACAGGAGCAAGACTTCTTGGACCGCTGCTT CCAGGAGATGCTGGATGAAGAGGACCAGGACTGGTTCATCCCAGCACGAGACCTGCCTCAGGCTGTGGGG CACTTACAACAGCAGTTAAATGGACTATCAGTTGGTGACAGTCATGAATCTGAAGACATTTTGAGCAAAA GTAACCTGAATCCAGATGCCAAGGAGTTTGTTCCAGGAGTGAAGTACTGAGCTGTTGGAAGCTTCCAGAA GGACTTGTCTTGTCCCCGTACCTGGGGCCAGCAATGCACAAAAAGGGCGGAGCTGAAGGGGGGTGGCAAG GGTGTGCAGCCAGGGCGGGTTGGGTTGGGAAGGTGGTTTAACTGGATATTGTGACTCTGAGTAACTAATG TGCGAGGTACAACTCTCCAAGCCTGAGCATGGCTCTTCTGTTGTGCAGACTTGCTCAGAGCAGTTTCCAA TTTATTTTATTTTTGATGAGCTCTTTGGAGTTAAGGAACTGCGATTGTTTTTCCCTTGTAAGGAATGTTG TCTGCATTTTAGCAAAATAAGCTGACCAAGGAAAACAGTATTTAAGAATAATTTTCCCTGCTGTGTACCT ACCAGCAGCAGGGTTCCTGGAGAACTTAAGGAGTTTGCAGCTGGGTGATAAGGGGGAAAACATGAGATAC AAACCAGATATAACATGCAGCTCCCATCCCCTGATATTTCCATGATGAACTGGGATACCAGTGGCCCATA AAGCTCTTGTCTCCTGTCCTGAACTCAGCTCAAGAGAACACAGATTTGAAAGTTCTTGGATGACTGTAAC TTTGGATGGATGTGAGTTGTATATCAGAAAAGCAGGGCTTGATGGTGATTGTCATTAGTGACTGCTATCC TATCATTAGTATGTAGCTATTGTCACTCATCAGGGTTGCTTTGCCTTCATGATCTTCATGGGGGCTGCTT CTGGCTTCTTTCCCCCCTCAGCTCTAAAAGACACTATTAGTCAAAGTCTGAGATGGGCCTTCAGTTGTGT TAGTTACACCTTGAACCTTTTCTGGACAGTTTTGTTTTCCTAAGTCCCTTCAATATCTGATTCTTAGGAT GTCTTAATTCCCTTATATTTTCTGTGTTTCCAGACACTAACAAGCTGCTTAGTTTGGGTTTTTCTCATGC CTTACAGAGTGATTTCCAGAAACTAAGGTGCTGAGGGGTCCTGCCCGAGAGCACAACAATCAGGCTTGCT ATGTACTTGAAGCTGTTAGTAGGTGTTACTGTTCCCACAGACCAGCTTTAGTGTCCTCATGGTCCTGCCT AGGTGCACTCTTAGTTCTAGCTCTAACTCACTAGGGTTTTTGTTGGTGTTCTGTGGAACTGAACTGCACC AGAAGCTGCCGCTGTGAGTCGAGGTGAAGGTGGTAGTGTCTGAGGGTGAGAGAACAAGTGTCCTGTCCTG ATCGTACTCCGAGGACAAGGGTCTTGGGAGGACTGATAGTGCTGTGGCTTCTGTGTGGCTATCACTGTGG TGAATGACAGGTCTGGAAAGATCATGCTGCTTCCAGCAGTCCATCAGATCAGTGTATAGATATGGGCAGA GACTGGAAGGAGAGGACTGAGGTCTGGAAAGTTGAAAGTTTCCCTTTTTCATATGAACTGGTTTGCTGAG TTTCCATTTTTTCTTGGTGCCATGGATAGAACCCAGAGTCTCACTTGTGCCAGGTTAGTCCTACCCTTGA GCTGTTCTCAACCCTCCCTTGATTTGAACACAGCCCCAGCAGTTAAAGACAGATGTCCCTCTTTTTCCTG TTTGAGTCATCACAAGGATGGCATCTTGTGGAAGTGGCCTCCCTCTCAGGTCACATCCTAGTGAGTAGCT CAGGGAACTTGTATGGCCAGACACACACATCAATTCAAAGCCTCTCCATCAAGAGAGAATGAAACTAGGA ACTCCTTCTGGAATGACTTTATTCCTGTTTAAGCTTTTAGCTTGGCTTGTCACCTCTAATAAGGAGAGGA GGAGGGTATGTGTAGGACATTTAAAAAGATTCTTTCTCTTGTAGTCTCCATGAGGTCAGGCTGTATCAGG ATTGCAGGAAGATCCTTCAGAATCTCTGTTTGCGTAGTGCACCTTCCTCTGAGCACCTATGGAAAGCTTA TGCAAGCCTGAGGCAGCTTTGTTCCATCCTTCAGTTTTGCTCTTTGGAAGCCAAGCTGCTAGGAATGGTC CAAGAGGCCTGCCTCCCTCCCGCTGTGCTGGCTCATTTTATTTGGATGTCATTCTTTATCCTGCTTTCAG GGGTGTATGCTGTTGGGTATACCTGGAATCCAAGAGCTAGTGTGTTGAGTCAACCTCAGAGGCAGGGTGC TGACTGTAGGCTTGGGATGCAGCCAGTGAACTGGCCCTTAGTAATGTATGGAATGACTTATTCCTGTGAC CCAACTCTTTTACTATCCAGTGTTGTGAGGAAGCACAATTGTACCCGAGACTACAGACTTAGCCCCAATT ACAAAAAAGTTTTGGGTTGTTTTTTTTTCCTTGGAATTCTTAGATTGCTGTGTAAATTCTTATACAGCAA TAGAAAGCATTCTAAATAGCTGCTAGTCTGCCTGGTCTCAGTATTCATTTATTAGAGACTGAGTTCTGTG ATTATTTGACCCTATTAACTTTGGGGGGTGGGGAGGAAGGAATTATTCATGAATTTTGGACTACATCAGA AATCTGAGATATCCAAAGCCTTGGTACTGTAGCTCAGGGTCAGGGGCCAGTTTCTATCTGTAGTGTACCT GACTTATGGTTATCATATAAACAAGCCAGGAGATATTCTAAGGAGAAGATTGGGTCCTTTTTATTGAACC CAGGACCTCACATGTGTTATGCAAGCACTACACCAAGCTGTACTCCAGGCCCGTGAAAGGTTGAGACTGG GTTAGCACTATTTCCAAAGTCACAAGACCTATGATTGCTTCATTAAGATTGTAAGGTTAACCATGGCTTA AAAATAAATGGTTTTGGTGAACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAG
Notes:	The probe template was PCR amplified from IMAGE:4511542 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4511542 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12237 same embryo
	EMAGE:12239 same embryo
	EMAGE:12238 same embryo
	EurExpress:euxassay_007045 same experiment
	MGI:4827029 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source