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EMAGE:12265

Ppp2r5b protein phosphatase 2, regulatory subunit B (B56), beta isoform ( MGI:2388480)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:12265 EMAGE:12265 EMAGE:12265 EMAGE:12265 EMAGE:12265
"Pseudo-wholemount" of euxassay_007020. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_007020_01 euxassay_007020_02 euxassay_007020_03 euxassay_007020_04
EMAGE:12265 EMAGE:12265 EMAGE:12265 EMAGE:12265 EMAGE:12265
euxassay_007020_05 euxassay_007020_06 euxassay_007020_07 euxassay_007020_08 euxassay_007020_09
EMAGE:12265 EMAGE:12265 EMAGE:12265 EMAGE:12265 EMAGE:12265
euxassay_007020_10 euxassay_007020_11 euxassay_007020_12 euxassay_007020_13 euxassay_007020_14
EMAGE:12265 EMAGE:12265 EMAGE:12265 EMAGE:12265 EMAGE:12265
euxassay_007020_15 euxassay_007020_16 euxassay_007020_17 euxassay_007020_18 euxassay_007020_19
EMAGE:12265 EMAGE:12265
euxassay_007020_20 euxassay_007020_21

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:12265Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
12265_wholemount_strong.wlz
12265_wholemount_moderate.wlz
12265_wholemount_weak.wlz
12265_wholemount_possible.wlz
12265_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:12265_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
brain
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21
facial vii ganglion
strong strong
regionalstrong expression: see section 05 06 07 08 18 19 20 21
glossopharyngeal ix ganglion
strong strong
regionalstrong expression: see section 07 18
trigeminal v ganglion
strong strong
regionalstrong expression: see section 04 05 06 07 08 09 18 19 20 21
vagus x ganglion
strong strong
regionalstrong expression: see section 08 09 18
vestibulocochlear viii ganglion
strong strong
regionalstrong expression: see section 07 08 18 19
trigeminal v nerve
strong strong
regionalstrong expression: see section 10 17
spinal cord
strong strong
regionalstrong expression: see section 09 10 11 12 13 14 15 16 17
cervico-thoracic ganglion
strong strong
regionalstrong expression: see section 10 11 12 13 17
cervical ganglion
strong strong
regionalstrong expression: see section 09 17
thoracic ganglion
strong strong
regionalstrong expression: see section 13 14 15 16 17
dorsal root ganglion
strong strong
regionalstrong expression: see section 08 09 10 11 12 13 14 17 18
neural retina
strong strong
regionalstrong expression: see section 01 02 03 04
nasal cavity olfactory epithelium
strong strong
regionalstrong expression: see section 08 09 10 11 12 13 14 15 16 17 18
tongue
strong strong
spottedstrong expression: see section 15
stomach
strong strong
spottedstrong expression: see section 03 04 05 06 07 08 09 10 11 12
midgut
strong strong
spottedstrong expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T8411
Entity Detected:Ppp2r5b, protein phosphatase 2, regulatory subunit B (B56), beta isoform ( MGI:2388480)
Sequence:sense strand is shown

>T8411
GCGCCGCCGCCCAGCCCCAGCGCCCCGAGCGGCAGAACCACCGCTAAGGACCCTTGAACCGTCGTCTCTT
CCACCGAGGCAACCTCTAGGGTCGGCGACCAAAGTCTGGGTTTCTGAGAAAAGAGCCAGCGTGGGAACCC
TGACTGGAACTCTTCTGGATCCCCAGGAAAGACCTGAGCCATTGCCTTTCTACCTTACCTGCCCCCCCAG
GACTGGGCAGTTGCCAGAGGCCCTGGGGGGGTCAGGACTGTGGCCGTGCCTCCCCCACCCCCTGAAACAT
GCTGGACAGGATGGGTCCCAGTTAGTGTGTGTGGCCTCATTCAACTTACTCCAGACCCACAAGGAGCCCC
CTGGGCTCTGAAAGATTGACCCGTGTTGTCCACTGGCCATGGAGACGAAGCTGCCCCCTGCGAGCACCCC
CACAAGCCCCTCCTCCCCAGGGCTGTCTCCAGTGCCACCACCAGACAAGGTGGATGGCTTCTCCCGCAGG
TCCCTCCGCAGGGCCCGGCCCCGTCGCTCACACAGCTCTTCTCAGTTCCGCTATCAGAGCAACCAGCAAG
AGCTCACTCCACTGCCCCTGCTCAAAGATGTGCCAGCCTCTGAGTTGCATGAGTTGCTGAGCCGGAAACT
GGCCCAATGTGGGGTGATGTTTGACTTCTTGGACTGCGTGGCTGACCTCAAGGGGAAGGAGGTGAAGCGT
GCAGCCCTCAATGAACTGGTGGAATGTGTGGGTTGCACCCGGGGTGTGCTCATCGAGCCCGTCTACCCAG
ACATCATCCGCATGATATCAGTAAATATCTTCCGGACCCTGCCACCCAGCGAGAACCCTGAGTTTGACCC
TGAAGAAGATGAGCCCAACCTTGAGCCCTCGTGGCCACATCTGCAGCTGGTATATGAGTTTTTCCTGCGT
TTCTTGGAGAGTCCAGATTTCCAGCCCTCTGTGGCCAAGAGATACGTGGATCAAAAGTTTGTCCTAATGC
TCCTGGAGCTATTTGACAGCGAGGACCCCCGGGAACGTGAGTACCTCAAGACCATCTTGCATCGGGTGTA
CGGCAAGTTCCTGGGTCTCCGGGCCTACATCCGCAAACAGTGCAACCACATCTTCCTCCGGTTCATCTAC
GAGCTGGAACACTTCAATGGTGTGGCTGAGCTCTTAGAGATCTTAGGAAGCATCATCAATGGCTTTGCGC
TGCCCCTGAAGACTGAGCACAAGCAGTTCCTGGTTCGAGTCCTGATCCCCTTGCACTCTGTCAAGTCACT
ATCTGTTTTTCATGCTCAGCTGGCATACTGTGTGGTGCAGTTCCTGGAGAAGGATGCAACCTTGACAGAG
CATGTTATCCGGGGGCTTCTCAAATACTGGCCTAAAACCTGCACCCAGAAGGAGGTGATGTTCCTGGGGG
AGATGGAAGAGATTCTTGATGTCATCGAGCCCTCCCAGTTTGTGAAGATCCAGGAGCCCCTCTTCAAGCA
GGTGGCTCGCTGTGTCTCCAGCCCCCATTTCCAGGTTGCAGAGCGGGCTCTGTATTTCTGGAACAATGAG
TACATCCTGAGCCTCATTGAGGACAACTGCCACACTGTGCTGCCTGCGGTATTTGGGACCCTCTACCAAG
TGTCCAAGGAGCACTGGAATCAAACCATCGTGTCCCTGATCTACAACGTGCTCAAGACTTTCATGGAGAT
GAACGGAAAGCTGTTTGACGAGCTCACAGCCTCCTACAAGCTGGAAAAACAACAGGAGCAGCAGAAGGCC
CAGGAGCGGCAGGAGCTATGGCGAGGCTTGGAGGAACTGCGGCTACGCCGGCTACAGGGGACCCAAGGGG
CCAAGGAAGCCCCCGTCCCACGGCCTACGCCCCAGGTGGCTGCCAGTGGGGGTCAGAGCTAGATACACCT
AGAACAGGAGATGCTAAACCCAGAGCTATCAGCCCCTCCATCCCTCTGCCCAGGGGCCCAGTGAGGCCCA
CGCCTCCCCGTGGCCTTGCCGGAGTGGCTCTAGGACTCCCTACCAGCCCCGTGGGAACAGCTTTCACGGA
GGGGGAAACAAGAAGGCAAGATGGTAGTCTTGGCAGCAGAACTCTCAGGCCTTTGTGGCAAGATTCTGGC
AAGACTAGACCAGGGCAAGTATGCAACTGGGAAGCTGCCATCAGGGATCCTCCCCTGCCCTACACAGCCA
GGCTCCAGGCGGCAGCTAGGCCTCTACCCCTGCTCCCAGCCTGGGGCATGGACACTCAGCGCCTGGCCAG
GGCCTTCCTCATCCCCACCATGGGGGCACAGTCTATTTATTCTGCCCAGCTCACCCCCAACAAGACCTTG
TCCAGGGACATTCTCCTATCCTCTCCCTGGCCCTGACTCCCTTATCCCCTTTTTATTTATTGGGCAGGGG
GAGGGGTGAGGGCACAGGCAAGAAGATTCACATTGTCCTGGGGTGAGGGGGGAGGTCACAGTAATCATGG
TCTGCCCCCTTCACCTGGCTGGGGGCAGATTTAATAAAGAGCGAAACTCAAAAAAAAAAAAAAAAAAAAA
AAAA
Notes:The probe template was PCR amplified from IMAGE:5687054 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5687054 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:12260 same embryo
 EMAGE:12261 same embryo
 EMAGE:12263 same embryo
 EMAGE:12264 same embryo
 EMAGE:12262 same embryo
 EurExpress:euxassay_007020 same experiment
 MGI:4827387 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS