EMAGE mouse gene expression spatial database

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EMAGE:12368

Ppcdc phosphopantothenoylcysteine decarboxylase ( MGI:1914062)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007003. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007003_01	euxassay_007003_02	euxassay_007003_03	euxassay_007003_04

euxassay_007003_05	euxassay_007003_06	euxassay_007003_07	euxassay_007003_08	euxassay_007003_09

euxassay_007003_10	euxassay_007003_11	euxassay_007003_12	euxassay_007003_13	euxassay_007003_14

euxassay_007003_15	euxassay_007003_16	euxassay_007003_17	euxassay_007003_18	euxassay_007003_19

euxassay_007003_20	euxassay_007003_21	euxassay_007003_22	euxassay_007003_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12368_wholemount_strong.wlz

12368_wholemount_moderate.wlz

12368_wholemount_weak.wlz

12368_wholemount_possible.wlz

12368_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12368_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9439
Entity Detected:	Ppcdc, phosphopantothenoylcysteine decarboxylase ( MGI:1914062)
Sequence:	sense strand is shown >T9439 CTGGAGCTGGACTCTCCTGTTCTTGCCACATTTGACTGAAGAAGGACAACGGGGTCAAGGTTTGAGGACT GACAGATTCTAGACCCAGGCTTCCTCAGCCCCCAGAAAGCCACCAGACCTCACATGGAACCAAAGGCCCC ATGCCCAGCCGCCGTCCCCTCAGAGGAGAGGAAGTTCCGTGTTCTTGTGGGCGTCACTGGCAGCGTGGCC GCTCTGAAGCTGCCTCTCCTGGTATCTAAGCTGTTGGACGTTCCTGGGCTGGAAGTCACAGTGGTAACAA CAGAGAGAGCCAAACATTTCTACAGCCCTCAGGATGTCCCCGTCACCCTCTACAGTGACGCTGATGAATG GGAGATGTGGAAGCGCCGTTCTGACCCGGTTCTCCACATTGACCTGCGGAGGTGGGCTGACCTCATGCTA GTGGCTCCCCTCGATGCCAACACTCTGGGGAAGGTGGCCAGTGGCATCTGTGACAATTTGCTTACCTGTG TCATCCGGGCCTGGGACCTAAACAAGCCCCTACTCTTCTGCCCCGCCATGAACACTGCCATGTGGGAGCA TCCTCTCACCGCACAGCAGGTAGCCCAGCTCAAGGCCTTTGGCTACGTGGAGATTCCCTGTGTGAGCAAG AAGCTGGTGTGTGGAGACCAAGGTCTAGGAGCCATGGCTGAAGTGGAGACCATTGTGGCTAAGGTGCAGG CCGTGCTCTCCCAGCATGGTAGCATCCAGCAGAGTTGACCTGGGATTTCTGCCGTGATGCCCCTGCACCC AGTGAGTTCAGCCTGAGCTGTTGAAGAAGGATATCAGCACAGTATGAAGCGCTTGTGCTGGTTGACGAGG GGCTAGCCTGAACCTTCTTGGGATTTCCCTTCAGTCTAACCCTCTTCAAGCTACACGGAACCACAGACCC AGGTCCTACCTTCTTTCAACCAAGAGATGCCTGCTTCCTGGAGGCCCTCCCATTTCTTCTGGGATAGCCC AAATAGACTAGGCAAGCACGCTGCTACTGGAAGGGTAATTGAATGACTGAGGGACCTCCTGCCTGGGAAT TGCTTTTAGACACACCATGGATGAAGCTGGGCATGGAGGCTCACATCTGTAATCCTAGCACTCAGAAGGC TGAGGCAGGATTGCTACAACTTTGAAGTTGGCTTGGGTTACATAGTAAGCCAGGCCTTTGAGTGTGATAG CCTATATCAAAACAAAAGCAACAAGAAGAAACACCATAGCTCAAGATCCCTGACATAGCAGGACACAGCA GGCTCCTGGTCAGGCTGGAAGCATCCAGTCTTTGGTGCCCAGGTGTCGCACACAGTCAAGAAGTCTGTGA GGTGGCCAAACTCTTCAGATTTCCCACAGTCTGTACTGAGAGTAGGGGCACGAACAGGTCTGGGAAATAA AGAAAAGTGACCTTTTAGGAGACTGTGTCCTTCTTTACATCCTGTCTGTGGCCTCAGAGGTCAGGGGCAT GGCCATGTGAGGCCAGAGAAGGTTCCAGGCTGACGACTGACTAGCACGCCCACATTCCTGTGCAGTCTCA AAGCAGACAGGTCAGATTCAGATCTCAATCCCACCTCCAGACCCAGACTGACCCTCGTTTTTCCATCTGC TGGGTCATGCGTCCATAACAGAGCTGCCGAGAGGATTAAGTGAGGCAGATGTGAAAAGTGCATTTGTCTC TTACACCCACAGGGGATACACGCTTGCTCCCAGCAGCTCCTGAAGCCAGAACGCCATACATTATGACGTC TTTCTTTACATAAACTGTGACAAATTTTAATTTATAAATTAGGCCCAGTAAGAGATCAGCAACAATAATA AAATAGAACAGTTATTCACTTTGTTCTGTAATAACTTATTTTCCATGCTGAAAGAAAAATAGAACAATTA AAAATATAATAGAGCCTGGCAAGATGGTTCAGTGTGTAAAGGTGTTTGCCACCTTGTGTCACCAAGCTTC ACAGTGTGAGTTTAATCCCTGGGAGTCACGTGGCGGAAGGAGAGAACCAGCCTCTGGAAGGTGACCTCTG ACCGCCACCTCCACTCTCACACTGTCACAGGTACTATCTGCATGTGTACACACATACAAAACAGATACAG TAAATATTTAAAAACTTGTGTGAACTCAACATACAACTTTAAAATTGTATCAAGTCAAGAACTTAGACTT TCTGTTGTTTTGTTTTGTTGTCTCGAGACAAGGTTTCTCTTTGTATCCCTGGCTGTCCTGAACTTCCTCT TTATACCAGAGTAGCTTGGAACTCGGAGAGCCTCCCGTCTCTGCTTCTCGAGTGCTGGGATTAATAGTGT GTACCATCACACTTGGCCCAGAACATCAAAGAAGGTTGATGCTTCTCTGGCTTCCCCGAGTTGTCTGCGT CCCGAGTCGTGGGCTGTTCCTGAGGTGAGAGGTTGCTTGAGCGCAAGGGCTATGCACCAGTGGTACTTGA TTTGTAACGCTGCTGCAGTAGCGGACTGTCAGTCATATTCAGCACGGACATGCTAGACTAAGTGATGATT CCTGTCTGGGTTGAGATGGAACAGGAAGTGTGGTATTTCGTGCTCCTCAGCGCAGCGTGTGACTTAAACT ATGGATCGTTTTAATGAACTTTGGTATTTCAGGCTACGGTTGACCAGGTAACAGAGAGCAACATTGCACG TAAGTGGAGACTTTTCTAACTGCTATGGAATACCCAGAATAATAAATGTCAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30603115 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30603115 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12370 same embryo
	EMAGE:12371 same embryo
	EMAGE:12367 same embryo
	EMAGE:12366 same embryo
	EMAGE:12369 same embryo
	EurExpress:euxassay_007003 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source