EMAGE mouse gene expression spatial database

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EMAGE:12373

Pcdhga8 protocadherin gamma subfamily A, 8 ( MGI:1935221)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_007167. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_007167_01	euxassay_007167_02	euxassay_007167_03	euxassay_007167_04

euxassay_007167_05	euxassay_007167_06	euxassay_007167_07	euxassay_007167_08	euxassay_007167_09

euxassay_007167_10	euxassay_007167_11	euxassay_007167_12	euxassay_007167_13	euxassay_007167_14

euxassay_007167_15	euxassay_007167_16	euxassay_007167_17	euxassay_007167_18	euxassay_007167_19

euxassay_007167_20	euxassay_007167_21	euxassay_007167_22	euxassay_007167_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12373_wholemount_strong.wlz

12373_wholemount_moderate.wlz

12373_wholemount_weak.wlz

12373_wholemount_possible.wlz

12373_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12373_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9138
Entity Detected:	Pcdhga8, protocadherin gamma subfamily A, 8 ( MGI:1935221)
Sequence:	sense strand is shown >T9138 ATCGAATTCTCAAGGCTGATGAAATCAGACCGGCAGATTCCGGAGTCCAACTGCGTAACTGCGGAGACAT TCTGTCCCTAAATGTGTGACTCTCCAGCTTTCCGTAAGATTATTTCCGTCGCCACAAGCGAAGGTAATCC AGCCACCTATGCCCGGTGAAGACTGAGGATTCTTTTGCAAATGAACAATGGCTGCTTCCAAGAATCAACA CAGGCATGGCCGGCTGGTGCTGCTGTGTACGCTGATGGGGACGCTGTGCAAGATCAGCGTAGGACAGATT CGCTACTCCGTGCCAGAAGAGACAGACAAAGGCACTGTCGTGGGCAACATCTCCAAGGACCTGGGGCTGG AGCCCCGCGAGCTGGCGGAGCGCGGGGTCCGTATCGTCTCCAGAGGTAGGTCGCAGCTTTTCTCCCTGAA TCCGCGGGGCGGCAGCTTGGTCACCGCAGGCAGGATAGACCGGGAGGAGCTGTGCGCTCAGAGCACGCCT TGTCTTGTAAATATCAACATCCTAGTTGAGGAGAAAGGAAAACTCTTTGGGGTAGAAATTGAAATAACTG ATATTAACGATAATAATCCGAAGTTCCATGTTGGAGATCTGGAGGTGAAAATTAATGAAATCGCTGCCCC TGGAGCACGGTATCCACTCCCAGAAGCGGTTGACCCAGATGTGGGCATAAACTCCCTCCAGAGTTACCAG CTCAGCCCCAATCGCCACTTCTCTCTCCACCTGCAAACTGGAGACGACGGAACTATAAACCCAGAGCTGG TGCTAGAGCGCACTCTGGACCGAGAGGAAGAACCCACTCACCACCTGGTCCTCACGGCCTCCGATGGCGG CGAGCCACGCCGCTCCAGCACAGCGCTTATCCAGATCACAGTGTTGGATACTAACGACAATGCCCCTGTT TTTGACCAACCGGTTTACCGCGTGAAAGTCCTTGAGAATGTGGCCCCAGGCACCCTGCTGCTCACAGTAA GAGCTAGCGACCCGGATGAGGGCGTCAATGGGAAGGTGACATACAAATTCCGGAAAATTAATGAAAAGCA GTCGCTGTTGTTCCATCTTCATGAAAACACGGGAGAAATGACAGTAGCGAAAAATCTGGACTATGAAGAA TGTTCATTGTATGAAATGGAAATACAGGCTGAAGACGTAGGGGCGCTTCTGGGGAGGAGCAAAGTGATAA TTATGGTAGAAGATGTAAATGACAATCGGCCAGAAGTAACCATTACATCCTTGTTTAATCCGGTATTGGA AAATTCTCTTCCTGGAACAGTAATCGCCTTCTTGAATGTACATGACCAAGACTCTGGAAAGAACGGCCAA GTTGTCTGTTACACGCATGATAACTTACCCTTTAAACTAGAAAAGTCAATAGATAATTATTATAGATTGG TAACATGGAAATACTTGGACCGAGAAAAAGTCTCTACCTACAATATCACGGTGATAGCTTCGGACCTAGG AGCCCCACCTCTGTCTACTGAAACTTATATTGCCCTGACTGTGGCAGACACTAATGACAACCCTCCTCGT TTTTCTCACACTTCCTACACAGCCTATCTCCCAGAGAACAACCTGAGAGGTGCCTCCATCTTCTCATTGA CTGCACATGACCCTGACAGCCAGGAGAATGCCCAGGTCACTTACTCTGTGTCTGAGGACACTATACAGGG AGTGCCTTTGTCCTCTTACATCTCCATCAACTCTGACACTGGCATCCTGTACGCACTGCAATCTTTTGAC TTTGAGAAGATACAAGACTTGCAGCTATTGGTTATTGCCACTGACAGTGGAAGCCCACCTCTCAGCAGCA ATGTGTCCCTGAGCTTGTTTGTGTTGGACCAGAATGACAATGCACCTGAGATCCTATACCCCAGCTTCCC CACAGATGGTTCCACTGGCGTAGAATTAGCGCCCCGCTCTGCAGAGCCTGGATACCTGGTGACCAAAGTG GTGGCAGTGGACAAAGACTCGGGACAGAATGCTTGGCTGTCCTACCGTCTGCTCAAGGCCAGCGAACCTG GGCTCTTCTCTGTAGGGCTTCACACGGGTGAGGTGCGTACAGCGAGGGCCCTGCTGGACAGAGATGCTCT CAAACAGAACCTGGTGATGGCTGTGCAGGACCATGGCCAGCCTCCTCTCTCAGCCACTGTAACTCTCACT GTGGCCGTGGCTAACAGCATCCCTGAAGTGTTGGCTGACTTGAGCAGCATTATGACCCCTGAGGTACCAG AGGATTCTGATCTCACGCTCCACCTGGTGGTGGCAGTGGCTGTGGTCTCCTGTGTCTTCCTTGTCTTTGT CATTGTCCTCCTAGCTCTCAGACTTCAGCGCTGGCAGAAGTCTCGCCAGCTCCAGGGCTCCAGAAGTGGA TTGGCCCCTGCACCTCCATCACATTTTGTGGGCATTGACGGGGTACAAGCTTTTCTACAAACCTATTCTC ATGAAGTCTCACTCACTTCAGGCTCCCAGACAAGCCACATTATCTTTCCGCAGCCCAACTATGCAGACAT GCTCATTAACCAAGAAAGCTGTGAGAAAAATGATTCCTTATTAACATCCATAGATTTCCATGAGAATAAG GATGAAGATGCTTGCGCTCCGCAAGCCCCGCCCAACACTGACTGGCGTTTCTCTCAAGCCCAGAGACCCG GCACGAGCGGATCCCAAAATGGTGATGAAACTGGCACCTGGCCCAACAACCAGTTTGATACAGAGATGCT GCAAGCCATGATCTTGGCCTCTGCCAGCGAAGCTGCTGATGGGAGCTCTACCCTGGGAGGGGGCGCTGGC ACCATGGGTCTGAGCGCTCGATACGGACCCCAGTTTACCCTGCAGCACGTGCCTGACTACCGCCAGAACG TGTACATCCCTGGCAGCAATGCCACGCTGACCAATGCCGCTGGCAAACGAGATGGCAAAGCTCCAGCAGG TGGTAATGGCAACAAGAAGAAATCGGGCAAGAAAGAGAAGAAGTAATATGGAGGCCAGGCCTTGAACCAC AAGGCAGCCTCCCTCCCCAGCCAGTCCAGCTCGTCCTTACTTGTACCCAGGCCTCAGAATTTCAGGGCTC ACCCCAGGATACTGGTAGGAGCCAGGGCCATGCTCCCCGTTGGGAAACAGAAACACGTGCCCAAGCCAAC ACCCCCTCTCTATACCCTAGGGAGTTGAATATGCAAAGAGAGTTCTGCTGGGACCCCCCTATTCAATCAG TGATTGTACCCACATGGGTAGCAGGGTTTGTGTGTGTGGACATACACACACACACACACACACACACACA CAAACCATGCCACACTCCCTTAGTTACAGCTGAACTCCTCCATCTTCCAACCCAGGCAGGCTTATCCATC TCCTGCCGCCCTCTCCCCACCCCACCCACTCCTTCAGTCCCTCTCTTTGAGCAAGGTAGCTGGGCTGTTG GGGTGACCGGTGAGCTAAGCCGGCTCCTAGTTCTGAACGGTAGGGAGAGTATTACAATCTTTTGGCCTCT CCTTCAGTTCAGCTTTCCCCCAAAGCATGGTTTGGTACCAGTCTTCTCTTTTCCTTCCAGAGCCTGAGAC CAATGCTTAAGTTTTGGGGGAACAGGCACCCTCCCTCTGGTACTGATGATGCTTGCTGGATTTTGGGAAG GCATTTTGCTACTAAGCCTCTTTTCAATGCCTGGGGACAACTAGTCTTTTGTTTTTCATTGTTTGATGTT CCCACTGCATGCTGTGACTTCCCTACACCTCAAAGAAGAGACTCCTTTGCATGTCTGGAGACAGTAGGGG GTGGTAAACTAAAGGGAAGTTGAGAGTGTATACTGCTGGGAGTGGGGCAGTGGACAAAACAGCCTGTTAA TAGGGTCTTGCAAGGGGCTCTGTATGTACCCAGGGGACTGGCTCAATCCTCACATTCTAGCCATAGACAA ACACCAGGCCAGACCCCTCCATTCTGGTTCAGCCTGGGCAGCTTGGGCTGAGCCACCAGGACCAATGGAT TTAAGCTGACATTTCAGTCCAAGACGACGACTTCTAAGTGAGTTTAAGACCAGAGAGGAAAGAGGGGCCT CTGTGGGTGCTGGGTACTCCAGAGGTGCCCTTGGTGGGAGGACCAGTGGTCTTAGCAGGAAGGGGGGCCC AGCAAGGTCATTCTTGGACCCTGGGTCTAGTCCAGTAGCTAGAAAAAGGGACCAAGTGGCCATAAAGTCC CAGCCAATGATGGGGCTTTTCCAGTGGGCCCCTGTAGACCTCAAGCCCCTGGCCTCCACCTTACCAGGTG CCATTTCTTCTCAGAAGGCCACTGCCCAGGCCCCCAGGCCGCCCCCTTGTGGCCAGAGAACGGTTAAAGC CCCCCAGTGCCTCCTTGTGCATAGAACTTCCTCTGCCCTAGCCCTTACAGTAGTGTAGAAGATCCCCCTC CCCCGCTGGTGTAGAATAGCCAATAGTATAGTGTGGTGTGCTTTTACGTGATGGCGAGTGGGCAGCGGGC GGTGGGCTGTACACAGCCGTCTGTCCTTTGAATCTCAATCTGCCTGCGCGGCCCGTGCTGTGTTTTGTGC TGTGTCCACACGCTGCGGCGACCCCTCCCTGTACTGACTTCTCTATAAAAAGCTTCTCTTCGCATAGTCA CGTAGCTCCTGCCCATCCCCCTTCCGATATCTCTCGCAAGTTTTATACTCTAATATTTATATGGCTTTTT TCTTCGAAAATTAAAAATAAAAAAATGGTTTCTTCTGAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6825772 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6825772 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12376 same embryo
	EMAGE:12372 same embryo
	EMAGE:12374 same embryo
	EMAGE:12375 same embryo
	EurExpress:euxassay_007167 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source