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EMAGE:12387

Kdm5d lysine (K)-specific demethylase 5D ( MGI:99780)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:12387 EMAGE:12387 EMAGE:12387 EMAGE:12387 EMAGE:12387
"Pseudo-wholemount" of euxassay_008354. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_008354_01 euxassay_008354_02 euxassay_008354_03 euxassay_008354_04
EMAGE:12387 EMAGE:12387 EMAGE:12387 EMAGE:12387 EMAGE:12387
euxassay_008354_05 euxassay_008354_06 euxassay_008354_07 euxassay_008354_08 euxassay_008354_09
EMAGE:12387 EMAGE:12387 EMAGE:12387 EMAGE:12387 EMAGE:12387
euxassay_008354_10 euxassay_008354_11 euxassay_008354_12 euxassay_008354_13 euxassay_008354_14
EMAGE:12387 EMAGE:12387 EMAGE:12387 EMAGE:12387 EMAGE:12387
euxassay_008354_15 euxassay_008354_16 euxassay_008354_17 euxassay_008354_18 euxassay_008354_19
EMAGE:12387 EMAGE:12387 EMAGE:12387
euxassay_008354_20 euxassay_008354_21 euxassay_008354_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:12387Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
12387_wholemount_strong.wlz
12387_wholemount_moderate.wlz
12387_wholemount_weak.wlz
12387_wholemount_possible.wlz
12387_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:12387_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T9157
Entity Detected:Kdm5d, lysine (K)-specific demethylase 5D ( MGI:99780)
Sequence:sense strand is shown

>T9157
CTGATTTTTTAAGTTTTGGGGTTTGGTTTTTGTTGTTGTTGTTGTTTGTTTTGTTTTGTTTTCACCTTTT
GCTTTCACTTTACTGAAATTTTTTCTTCTTTTTCCTTTCCAGGAGTGTAAGAGTCCCCCTGAAGCTTTTG
GCTTTGAGCAGGCTACACAGGAGTATACTTTGCAGAGTTTTGGTGAGATGGCTGACTCCTTCAAGGCTGA
CTACTTCAACATGCCTGTGCATATGGTACCTACAGAAGTTGTGGAAAAGGAATTCTGGAGGCTGGTGAGC
AGCATTGAGGAGGATGTGACAGTTGAATATGGAGCAGACATTCACTCCAAAGAATTTGGCAGTGGGTTTC
CTGTCAACAATAGCAAATGGGACTTATCTCCTGAAGAAAAGGAATATGCTGCTTGTGGTTGGAATCTCAA
TGTGATGCCAGTGCTGGATCAGTCTGTTCTCTGCCACATCAATGCAGACATCTCAGGCATGAAAGTGCCC
TGGTTATATGTGGGCATGGTGTTTTCAGCATTTTGTTGGCATATTGAGGATCACTGGAGTTATTCCATTA
ACTACCTGCACTGGGGTGAACCAAAGACCTGGTATGGAGTACCTTCGCTAGCAGCAGAACACTTAGAGGA
TGTAATGAAGAGACTTACACCAGAGTTGTTTGACAGCCAACCTGACCTCCTGCACCAACTTGTCACTCTG
ATGAATCCTAACACTCTCATGTCACATGGAGTGCCAGTTGTTCGTACAAACCAGTGTGCAGGGGAATTTG
TCATTACTTTTCCCCGTGCTTACCACAGTGGCTTTAACCAAGGCTATAACTTTGCTGAGGCAGTGAACTT
TTGCACTGCCGATTGGCTACCTGTTGGACGCCAGTGTATTGAACACTACCGCCGACTCCGGCGCTATTGT
GTCTTCTCCCATGAAGAACTTATCTGCAAGATGGCTGCATTTCCAGAGAAGTTGGATCTAAATCTAGCAG
TGGCAGTTCACAAGGAGATGTTCATTATGGTACAGGAGGAGCGACGCCTACGAAAGACCTTGTTGGAGAA
GGGCATCACGGAAGCTGAGCGAGAGGCTTTTGAGCTACTCCCAGATGATGAGCGTCAGTGCATCAAGTGT
AAGACCACGTGTTTCCTATCAGCCTTGGCCTGTTATGACTGTCCAGATAGTCTTGTCTGCCTTTCCCATA
TCAATGATCTGTGCAAGTGCTCAAGGAATCGACAGTACTTACGGTACAGGTACACATTGGATGAACTGCC
TGCTATGCTACAAAAGCTGAAGATTCGAGCTGAGTCATTTGACAACTGGGCCAACAAAGTACAAGCAGCC
CTAGAGGTGGAGGATGGTCGGAAACGGAGTTTTGAAGAGCTAAGAGCATTGGAATCTGAGGCTCGTGATA
GACGATTTCCTAATAGTGAGCTGCTTCAGCGATTGAAGAAATGCCTAACTGAAGCAGAGGCTTGTATTTC
CCAAGTTCTTGGACTGATCAGTAACTCAGAGGACAGGTTACAAACACCGCAAATCACCCTTACTGAACTA
CAGCTTCTTCTCAAACAGATGGGCACTCTGCCCTGTACCATGCATCAGATTGATGAAGTTAAGGATGTTC
TTCAACAGGTGGAATCATACCAAATTGAGACCCGTGAGGCTCTAACTTCACTGCCTTACAGTTTAGAAAT
ACTTCAGTCCCTGATGGAAAAGGGTCAGCAGCTTCGTGTGGAAGTGCCTGAAGCTCATCAACTTGAAGAG
CTGTTGGAGCAGGCACAATGGCTAGATCAAGTGAAGCAGGCCCTGGCTCCCTCAGGCCAGAGGCACTCTT
TGGTTATCATGAAGAAGCTTTTAGTTATGGGAACCAAGGTAGCCTCCAGCCCTTCTGTGAACAAAGCTCG
AGCAGAGTTACAAGAACTGCTGACTATTGCAGAATGTTGGGAGGAAAAGGCCCATTTTTGCTTAAAAGCT
AGCCAAAAGCATTCTCCAGCCACTTTGGAAGTTATAATACGTGAGGCAGAAAACATCCCTGTCTACCTAC
CTAACATCCAGTCTCTGAAGGAAGCTCTGACTAAGGCACAGGCTTGGATTGCTGATGTGAATGAGATTCA
AAATGGTGATCACTACCCCTGTTTGGATGATTTGGAGGGCCTTGTAGCTGTTGGTCGGGATTTACCTGTG
GAGCTAGAGGAATTGAGACAGCTGGAGAATCAGGTACTAACAGCACATTCCTGGAAAGAGAAAGCTTCTA
AGACTTTTCTTAAGAAGAATTCTTGCTACACATTGTTGGAGGTTCTCTGCCCATGTGCAGATGCTGGTTC
AGTCAGCACCAAGCGCAGTCGGTGGATTGAAAAGGAGATGGGATTATACAAATATGACACAGAATTGCTG
GGGCTGTCTGCACAGGACCTCAGGGACCCAGGGTCTGTGATCATGGCCTTTAAGGAAGGGGAAGAGAAAG
AAAAGGAAGGTATCTTGCACTTACGTCACATCAACTCAGCCAAGCCCAGTCCAATGTCCTCATCGATGAA
TGCCTCTGCAACCTCCATCTGTATATGTGGGCAGGTCTGTGCAGGAGTGGAATCTCTGCAGTGTGATCTT
TGTCATGACTGGTTCCATGGACAATGTGTGACTGTACCCCACCTACTCAGCTCAGTAAGGGCCAGTCATA
CCTCATCTCAACTGCTAGCTTGGTGGGAATGGGACACCAAGTTCCTGTGCCCACTGTGCATGCGATCAAG
GCGTCCACGCCTGGAGACCATCTTGTCATTGCTGGTAGGACTACAGAGACTGTCTGTAAGGCTACCTGAG
GGTGAGGCCCTACAGTGCCTCACAGAGAGAGCCATTGGTTGGCAAGGCCGTGCCAGGCAGGCTCTAGCTT
CTGAGGATGTGACTGCTTTGTTGAAACAGCTGGAAAAATCCCGCCAACAACTACAGGATGAATTGAGACA
TAAGAAGCCCCCTACCTTGCCTTCAGGCTTTGCCTTTGACTGTCTCACAGAGAATAGTGGCAAGGATATC
CTTAAGGAAGAGGAGGAGTTGGTACTAAATGAAGAAAGGATTAAAAGTTCTGAGAAGATTGTTCCAAAGG
AAAGTTCATGTAAAGGAGATAAGGAACTGCTGCCTTCACTTTTATCCCAATTAACTGGCCCAGTACTGGA
ACTGCCTGAAGCCACCCGGGCACCCCTAGAGGAGCTTATGATGGAAGGAGATCTACTTGAAGTTACCCTA
GATGAGAACTACAGCATTTGGCAGCTGCTGCAGGCTGGGCAGAATCCCAATCTAGAGCGCATTCATACAC
TTCTGGAGCTTGAGAAGCCTGAAAACCCAGGAAATTGGTCAGAGGAACAGACACCAGAGAGGCGACGGCA
GAGGAGGCAAAAGGTGGTTCTGAGTAGAAAGGGTGAGGATTTTACTCAAAAAGAGCTAGAATCAAAAAGA
GTCAAGAGTTCAAGGATTAAGCCTAAGGAAGAAAAGTTTCAAAAGCCAATACTTGGGGATAATGTGCTGT
ACACACACCATACAGAACATACAAACATCTTAAAAGAGCACATAAACAGTGTACAAGGAAAGGATCCAAG
CCCTTCATCTTCTTTTCCTTCCTTAACACCTTTACTACACCTATCTTATTTTCATCAGCAAAAATTATAA
CAATAGTATGTGATTATAACAATAGTATGTGATTGTTCTTTTTTCCTACCTTAGTCTTTTCCATTCTCCC
TCCCTTGTTCTTTCTTTCCTTCTTCTTTTTCATTTCTTTCTGTTGGTCCCTTCCTTTCCTCCATTTTACT
CTATTCCTACAGCTTCCTCTGCCCTTAATCCCTCAGATACCCCCAAAGTATTTGTTTCTTATCTAGAGCT
TGACCACAAATTATTTTATCCTTACTCCTAATACATTTTTATGCCATAATTCCTAAGAAGTATTCCCTGT
ATGCGTTTCCCAAAATTTTACCATCAATATTCCTGGGACTAGCATAGATAAAGTGATGTTCTTAGTTATC
TCTATTCTAATCTGGAGAGACAAAAATAAAATTTGAGAATGATAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:6826863 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6826863 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:12390 same embryo
 EMAGE:12388 same embryo
 EMAGE:12389 same embryo
 EurExpress:euxassay_008354 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS