EMAGE mouse gene expression spatial database

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EMAGE:12442

Derl1 Der1-like domain family, member 1 ( MGI:1915069)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011215. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011215_01	euxassay_011215_02	euxassay_011215_03	euxassay_011215_04

euxassay_011215_05	euxassay_011215_06	euxassay_011215_07	euxassay_011215_08	euxassay_011215_09

euxassay_011215_10	euxassay_011215_11	euxassay_011215_12	euxassay_011215_13	euxassay_011215_14

euxassay_011215_15	euxassay_011215_16	euxassay_011215_17	euxassay_011215_18	euxassay_011215_19

euxassay_011215_20	euxassay_011215_21	euxassay_011215_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12442_wholemount_strong.wlz

12442_wholemount_moderate.wlz

12442_wholemount_weak.wlz

12442_wholemount_possible.wlz

12442_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12442_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8587
Entity Detected:	Derl1, Der1-like domain family, member 1 ( MGI:1915069)
Sequence:	sense strand is shown >T8587 AGCTTGTCCGAATTCGGTGGCGCCACGTCCGTCGGTCTCCGCCTCCTGCACAGCGGCTGCGCCTCCACTT TCCCTGGCCAAGCCGGCCGCGGAGCGCGGGGTGGGCACGGGAGGACGCGCGGCGCTGCGCAGCCGGTCAC CTGACGGGCGACCATGTCGGACATCGGGGACTGGTTCAGGAGCATCCCGGCCATCACGCGCTACTGGTTT GCTGCCACCGTTGCTGTCCCCTTGATCGGCAAGCTCGGCATCATCAGCCCGGCCTACTTCTTCCTCTGGC CCGAAGCCTTCCTCTATCGCTTCCAGATATGGAGGCCGTTCACTGCCACCTTTTACTTCCCCGTGGGCCC AGGGACTGGATTTCTGTATTTGGTCAATTTATATTTCTTATATCAGTATTCTACTCGGCTTGAAGCAGGA GCTTTTGACGGGAGGCCAGCAGACTATTTATTCATGCTTCTCTTTAACTGGATCTGCATCGTTATTACTG GCTTAGCAATGGATATGCAGCTGCTGATGATCCCTCTGATCATGTCAGTGCTCTACGTCTGGGCCCAGCT GAACAGAGACCTGATCGTGTCGTTCTGGTTCGGAACGCGATTTAAGGCCTGTTACTTACCTTGGGTTATC CTTGGATTCAACTATATCATTGGAGGCTCGGTGATCAATGAGCTCATTGGAAACCTTGTCGGCCATCTTT ATTTCTTCCTGATGTTCAGATACCCAATGGACTTGGGAGGAAGGAATTTTCTGTCCACACCTCAGTTTTT GTACCGCTGGCTACCCAGTAGGAGAGGAGGGGTGTCAGGATTTGGTGTGCCCCCTGCTAGCATGAGGCGA GCTGCTGATCAGAATGGCGGAGGCGGGAGACACAACTGGGGCCAGGGCTTCCGACTTGGAGACCAGTGAG GGGCAGCCCTGGCCTGCTCACCAGCCATGCCGAGCAGATGACAGTCTCCTCCCAGTGCTGGGCATGCTTA GTGGCCGAGCTCTTGCTGCCGCTCTTGGACCTGACCCACACTGAATGTAGTCTTTCAGTACAAGACACAT TTTTAAGTCCTCAAGGAAAATACAAGTGTTCCTCAAGTTTCATGATTCTCATTCAAGTCCTTACTGCTAT GAAGAACAAATATCAGCTGTGCAGATTTCAAAACTGACTCATTTTCTGGTGTCTTCTCTTCCTCCCCTTC CATCTGAATAATGAGCTGGGCAGGAGGGGTTGGTTAACCAAATCCAGTCAAAGAACCCTTTCCTCTCACT CATGTCCCCAGAGTTGCAGCTAGAGTTGTCCACAGAAGGGTTTTGCCTTTGACAAACTTGCTGACTTTTG CCAAAGCTTGGTCACAAAGAGCTGCTTCACACATGGCTCTCGGCCATCACAAGCAGGACTGTAACAGTAA GGAGAAGATGGAGCAGCTGAGGAAGCTCTTCCTCTGCTGTGGGGCCTGCATCCGCCTGTCCGCAGTGCCG CCATTTGCCGCCTTCTCAGATATTCATACCCAAAGGCACCACTGAGTCAGTGGTGGCCCAGACTGGGAGT GATGAGGGTTAGGACTCGTTGCTAGGCACTTTTCTCCTCTGAGCCATGTGATCAGAACCATAGTGGAGTT GCTCCTGACACAGGATGGGTTTAAATACCGCGATCCCCTTGGGTTTCTTCCGAGGGGTGAACTGACTGTG TGCCCAGAGCATTTGTGCTGCGCTGTGTTGTGGTAGGAGGTTCCAGATCACACTGGCCAGACACACGTCC CTGGAGGCAAGCTTCGGGACACTGATTCCGTTCCATCCTCGTTGCGGATGTGTGTTCACCGAGTCGGTGG AGAAAAGAGTGTCAGGCTCTAAATTTTTTGTTCATTGTTTTTTGTCCCAGGCCTTTTCTGGTCGAGTGTT TGTGTTAATGGCAAGGGAAGTACAACTCCTCAGATGTGTGTCGATCAGTTTTTAAGGCTAATAGAGGCAC ATCTGAATGAGTCACTCATAATTAGAGGTTCCAATAGCTTTAGGATCATTTGCGTTTGGAGGTCATTATT ATCTTGAGTCATGCATGTATACTCTTTGTGAATCAGACCTGCTCCCTTTTCATAGGTGGGCTTTTGCCAG CAGAGCTCAGCTTATCACTAAATAAAGTTTTCTGAAGGCTACAGTGCTTTATATAGTTACTTTATGTTGT CATTCTGAAATCAGGCTGCTGGCCGCAGTGTGGTGTAGCTGGTATGCTCTGAATGATGTCTGAAGGTTTT TGCTACTTGGGTTTTTCCTTTGAGGCAATGTGCTCTAACAGTGTGACTTGTCTTTCTCTGCGCATTAAAA CTGTGATGTATGTTATATTAGGAAGGGGGAGTTTGAAAAAGAGCTCTCCCTTTCTCCATATTTAATTTAT GTGATTAAACTGTCGGGTGGAGAGAGGCTGACCCTGAGCCGCCTGGTTCTCCTGCCGACCCGAGGCTGTG ATAAAATGTGTTTGTCTAAGATCCACAAGCCGATCTCCCTGTGCCGGTGCCTGTGCACTTCTGTGCAAGG AAGGTGGAGACTCACCTTCGGAAAGGACTGCCACTTGAGCGAGCGAGAGATTATTCTAATGTCCTGGTAC CCGGCTGGCTTCTGGGAGGACTGGCTTTTCGAAGGGTCTGCAAGAATGCAGAACTTTTCTGTACAAGGCA GATGTTCATAACAGTTTTTCCCCTGGACCTGATTAGGAAGCTGATAACTTTATTATCCTTCCAGGATAGT TTTACCAAAGTGAGCCGGTTTTGAGTGTTGACTTCACAAGGTAAGAATTCATAGTGGGTTCTCTTCATGA ATGTGAAAAAACAAACAAACAAACCTTATTTTGTACAGACAGCTTTCCTTTTTGTAACTAATCCTTTTTA CTGGTAAATATTGTAAATTAAAATGTGCAACTTGAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:5715726 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:5715726 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12443 same embryo
	EMAGE:12441 same embryo
	EMAGE:12439 same embryo
	EMAGE:12440 same embryo
	EurExpress:euxassay_011215 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source