EMAGE mouse gene expression spatial database

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EMAGE:12520

Gjd4 gap junction protein, delta 4 ( MGI:2444990)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011337. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011337_01	euxassay_011337_02	euxassay_011337_03	euxassay_011337_04

euxassay_011337_05	euxassay_011337_06	euxassay_011337_07	euxassay_011337_08	euxassay_011337_09

euxassay_011337_10	euxassay_011337_11	euxassay_011337_12	euxassay_011337_13	euxassay_011337_14

euxassay_011337_15	euxassay_011337_16	euxassay_011337_17	euxassay_011337_18	euxassay_011337_19

euxassay_011337_20	euxassay_011337_21	euxassay_011337_22	euxassay_011337_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12520_wholemount_strong.wlz

12520_wholemount_moderate.wlz

12520_wholemount_weak.wlz

12520_wholemount_possible.wlz

12520_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12520_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

upper arm muscle

weak

regional

weak expression: see section 01 02 03 04 05 21 22 23

upper leg muscle

weak

regional

weak expression: see section 01 02 03 04 05 06 07 18 19 20 21 22 23

diaphragm

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

forearm rest of mesenchyme

weak

regional

weak expression: see section 01

hand

weak

regional

weak expression: see section 02

foot

weak

regional

weak expression: see section 01 02 03 20

lower leg rest of mesenchyme

weak

regional

weak expression: see section 01 02 03 22 23

vertebral axis musculature

weak

regional

weak expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

tongue muscle

weak

regional

weak expression: see section 09 10 11 12 13 14

tail paraxial mesenchyme

weak

regional

weak expression: see section 05 06 07 08 09 10 11 12 13 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T37480
Entity Detected:	Gjd4, gap junction protein, delta 4 ( MGI:2444990)
Sequence:	sense strand is shown >T37480 CATGAGCTCAGATTCCACAGAGAGACTTCACTGGACCTGGGGGGCAAAAACACCCAGGCAGATGAACTCT CCTTGGCTACCCAGAGCCACCTGGCCAGACACAGTTCAGCCAGCAAGCCTCAAGCTCCATGCCGGCTGAC CACCTCAGGCAGTGCTCCCCATTTGAGAACCAAAAAATCTGAGTGGGTGTGAAAGCAGCCACGCAGAGCC CCAAAACCCTTTTTTTTCTAGCACCGTACTGCCCTTTGTACTCAAGGCAGGGTAAGAGGTTTCTTCATCT TGTATTCTTGCTGAGATCATTTTATATTTAACTAGAAATTTTATTTCCATGGTATAGAATCCCATGAAAA ACTATCCTACACTGTTCTAAAGAACCTGAGGTCAAGACGGTCCAGCTATCTTACTTTCTGTTAAACTGCT TGCTTGGGCAAACCTCCTCCTCCATCTAATCACTTTTATTGAACTGTTTGATTAAGAAAAACCTCCCTCT ACAGCTTTGGAGCAAGTCACCAAGGTGCCCTGCAGGGCCTAGGATGGCCTTGGTAGGGGTGGAATGACAT GGTTCCTACCCCTGAAACAGGG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 94169. Forward Primer - name:094169_F_cDNA_9430022F06Rik, sequence:CATGAGCTCAGATTCCACAGAG; Reverse Primer - name:094169_N_SP6_cDNA_9430022F06Rik, sequence:CCCTGTTTCAGGGGTAGGAAC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12521 same embryo
	EMAGE:12519 same embryo
	EMAGE:12517 same embryo
	EMAGE:12518 same embryo
	EMAGE:12516 same embryo
	EurExpress:euxassay_011337 same experiment
	MGI:4825065 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source