Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:12533

Mamstr MEF2 activating motif and SAP domain containing transcriptional regulator ( MGI:1921740)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:12533 EMAGE:12533 EMAGE:12533 EMAGE:12533 EMAGE:12533
"Pseudo-wholemount" of euxassay_011304. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_011304_01 euxassay_011304_02 euxassay_011304_03 euxassay_011304_04
EMAGE:12533 EMAGE:12533 EMAGE:12533 EMAGE:12533 EMAGE:12533
euxassay_011304_05 euxassay_011304_06 euxassay_011304_07 euxassay_011304_08 euxassay_011304_09
EMAGE:12533 EMAGE:12533 EMAGE:12533 EMAGE:12533 EMAGE:12533
euxassay_011304_10 euxassay_011304_11 euxassay_011304_12 euxassay_011304_13 euxassay_011304_14
EMAGE:12533 EMAGE:12533 EMAGE:12533 EMAGE:12533 EMAGE:12533
euxassay_011304_15 euxassay_011304_16 euxassay_011304_17 euxassay_011304_18 euxassay_011304_19
EMAGE:12533 EMAGE:12533 EMAGE:12533 EMAGE:12533
euxassay_011304_20 euxassay_011304_21 euxassay_011304_22 euxassay_011304_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:12533Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
12533_wholemount_strong.wlz
12533_wholemount_moderate.wlz
12533_wholemount_weak.wlz
12533_wholemount_possible.wlz
12533_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:12533_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: three stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T37446
Entity Detected:Mamstr, MEF2 activating motif and SAP domain containing transcriptional regulator ( MGI:1921740)
Sequence:sense strand is shown

>T37446
ATGACATCCTGGAAGACCAAGTGGAGCCAGATGGTAGGCGATTAGCTCCCACATTTAGGGGAGGAACTGA
ACTTGGGAACTCCAGGGTCTCGTCGAAGGAGAAGTGGGTTGGAAACAGCGCAGTGAGCAGAATCCATCCC
CCTAAAGCTCACACTCCTCCTGCAGACCTGCTGCCTCCCGTGCCCTTGGACTTCCCTGGCTCATTTGACC
TGCTGTCTCCCTCCCCGGACTCGGAAGGCTTCTCTTCCGTCTTCTCTTCTTCACTACCGTCCCCCACAAG
CTCCCTGTCCCCTTCTCCAAGGGCCCTCACTGATTCCTTGGACTGGCTGGAGGCCTTGAGTGGGGGTCCA
CCACTGGGCTCTGGGCCTCCCGGCCCCAGCATCTTCTCTGCGGACCTATCTGATCCCAGTGGCAGTCTGC
TCTGGGAACTGCTGCCAGATCCTTGGTGACAGACCCTTGGGTCTAAGGAGAGGTCATCTGCATCCACAGG
GACCCTCCAACTACAGAGTACTGATGCCCTCGTCCCCTCCTGACTCACCGCTGCTGCTGATATCTTATGT
GCCTGGACCTGCCTATGGGGCCTCTGTATGGCCCCTCCCAGGCCCAGTGGTTGGGGTTGGGGGGTTCATT
TGCTACTGGCCAGTGCATACAAGCTTGCTGCCTCCCTCAGAGACCTGCAGGTTGTGTGGCTGCCTGTCAT
CTGGGGATGTGAAACTTTGTGCTGTTTGAGGTCAGGAAAGGACCAGGTAAGAGTAGAAGGACCGCCCC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 67072. Forward Primer - name:067072_F_cDNA_5430432N15Rik, sequence:ATGACATCCTGGAAGACCAAGT; Reverse Primer - name:067072_N_SP6_cDNA_5430432N15Rik, sequence:GGGGCGGTCCTTCTACTCTTA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:12530 same embryo
 EMAGE:12531 same embryo
 EMAGE:12535 same embryo
 EMAGE:12534 same embryo
 EMAGE:12532 same embryo
 EurExpress:euxassay_011304 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS