EMAGE mouse gene expression spatial database

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EMAGE:12542

4930486L24Rik RIKEN cDNA 4930486L24 gene ( MGI:1922258)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011234. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011234_01	euxassay_011234_02	euxassay_011234_03	euxassay_011234_04

euxassay_011234_05	euxassay_011234_06	euxassay_011234_07	euxassay_011234_08	euxassay_011234_09

euxassay_011234_10	euxassay_011234_11	euxassay_011234_12	euxassay_011234_13	euxassay_011234_14

euxassay_011234_15	euxassay_011234_16	euxassay_011234_17	euxassay_011234_18	euxassay_011234_19

euxassay_011234_20	euxassay_011234_21	euxassay_011234_22	euxassay_011234_23	euxassay_011234_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12542_wholemount_strong.wlz

12542_wholemount_moderate.wlz

12542_wholemount_weak.wlz

12542_wholemount_possible.wlz

12542_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12542_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

adrenal gland

moderate

single cell

moderate expression: see section 05 06 07 14 15 16

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9948
Entity Detected:	4930486L24Rik, RIKEN cDNA 4930486L24 gene ( MGI:1922258)
Sequence:	sense strand is shown >T9948 GCAGCTGAAACAGATCTGGAGAACCCAAGATCCTCTGAAACTCAAGCCTGAGGAAATCGTCTGAGGAGCA GCAGACACCCAGGTGGATGAGACATGATCGCTGTTCTCTTCCTAGCCATCCTCTGCTTGGAAATCGACTC AACTGCTCCAACACTAGATCCCAGTTTGGATGTTCAATGGAATGAATGGAGGACAAAACATGGAAAAGCA TACAATGTGAATGAAGAAAGATTGAGGAGAGCTGTGTGGGAAAAGAATTTTAAAATGATTGAGCTGCATA ATTGGGAATACCTTGAGGGGAAGCATGACTTCACCATGACAATGAATGCCTTTGGTGATTTGACCAATAC AGAATTCGTGAAAATGATGACAGGCTTTCGAAGGCAGAAGATCAAGAGGATGCATGTATTCCAGGATCAT CAGTTTCTTTATGTCCCCAAATATGTAGATTGGAGAATGCTTGGCTATGTGACTCCTGTCAAGAATCAGG GTTATTGTGCCTCTAGTTGGGCTTTTAGTGCAACTGGATCCCTAGAAGGACAAATGTTCAAGAAAACAGG GAGATTGGTCCCTCTGAGTGAGCAGAACCTGCTGGACTGCATGGGATCTAATGTTACCCATGACTGCAGT GGTGGCTTCATGCAGAATGCCTTCCAATATGTGAAAGACAATGGCGGCCTCGCAACTGAGGAATCCTATC CATACATAGGACCAGGTAGAAAGTGCAGGTACCATGCTGAGAATTCTGCGGCTAATGTCAGAGATTTTGT GCAAATCCCAGGACGCGAGGAAGCCCTTATGAAGGCAGTGGCTAAAGTGGGGCCCATCTCTGTTGCAGTT GATGCCAGCCATGATTCCTTCCAATTCTATGACAGTGGCATCTATTATGAGCCACAGTGCAAAAGGGTCC ACCTAAACCATGCTGTTCTGGTGGTCGGCTATGGTTTTGAGGGAGAAGAATCAGATGGCAACAGTTATTG GCTGGTCAAGAACAGCTGGGGTGAGGAATGGGGCATGAAGGGATACATAAAGATTGCCAAAGACTGGAAC AACCACTGCGGGATTGCTACACTTGCCACATACCCCATCGTCTGAGCTGCTGCACAAGAAGGAAGGAGAG GACTGGGAACGGAATGTACTGAGCAGGGTTTCTCCTCTTACAGACCAGCCTCTGCTCTGGGGGCAGAGGA GAGAGGCAGCTGAGTCGCAGAGGAGCCACACTTGATGTGAATTAAACTCATAAACATTATGCCACTAACA GCTTTTATTAGTGACTCTCTTATTTAAAAAAACCTGAATTTTCATTTTAAAATGCTGTACAATTTTACAT ATTCAAAATAAAGCTTGATTTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30281034 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30281034 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12541 same embryo
	EMAGE:12540 same embryo
	EMAGE:12543 same embryo
	EMAGE:12538 same embryo
	EMAGE:12539 same embryo
	EurExpress:euxassay_011234 same experiment
	MGI:4822729 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source