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EMAGE:12548

Fsd2 fibronectin type III and SPRY domain containing 2 ( MGI:2444310)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:12548 EMAGE:12548 EMAGE:12548 EMAGE:12548 EMAGE:12548
"Pseudo-wholemount" of euxassay_011369. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_011369_01 euxassay_011369_02 euxassay_011369_03 euxassay_011369_04
EMAGE:12548 EMAGE:12548 EMAGE:12548 EMAGE:12548 EMAGE:12548
euxassay_011369_05 euxassay_011369_06 euxassay_011369_07 euxassay_011369_08 euxassay_011369_09
EMAGE:12548 EMAGE:12548 EMAGE:12548 EMAGE:12548 EMAGE:12548
euxassay_011369_10 euxassay_011369_11 euxassay_011369_12 euxassay_011369_13 euxassay_011369_14
EMAGE:12548 EMAGE:12548 EMAGE:12548 EMAGE:12548 EMAGE:12548
euxassay_011369_15 euxassay_011369_16 euxassay_011369_17 euxassay_011369_18 euxassay_011369_19
EMAGE:12548 EMAGE:12548 EMAGE:12548 EMAGE:12548 EMAGE:12548
euxassay_011369_20 euxassay_011369_21 euxassay_011369_22 euxassay_011369_23 euxassay_011369_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:12548Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
12548_wholemount_strong.wlz
12548_wholemount_moderate.wlz
12548_wholemount_weak.wlz
12548_wholemount_possible.wlz
12548_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:12548_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
hindlimb digit 2 phalanx
moderate moderate
regionalmoderate expression: see section 05
hindlimb digit 3 phalanx
moderate moderate
regionalmoderate expression: see section 05
hindlimb digit 4 phalanx
moderate moderate
regionalmoderate expression: see section 05
tarsus
moderate moderate
regionalmoderate expression: see section 05
fibula
moderate moderate
regionalmoderate expression: see section 05
tibia
moderate moderate
regionalmoderate expression: see section 05
femur
moderate moderate
regionalmoderate expression: see section 09 21 22
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T37492
Entity Detected:Fsd2, fibronectin type III and SPRY domain containing 2 ( MGI:2444310)
Sequence:sense strand is shown

>T37492
ATACAGCTGCAGCCTAGACACAGCTATACCATCTATGTGCGAGCCCTCAACGTGGGAGGCACCAGTGCCA
GAAGTGAGCCTGCTACAGTGCACACCACAGGAAGCTACTTCCAATTGAACAAAGACACCTGCCATCCCTG
GCTGACCATTTCTGAAGATGGGTTTACGGTAGTTCGAAGTGAGAAGAAAAGTTTCAGAAAGGAGCTGCCG
CCGAGCAAGACGCAATTTACCAGGTGTGTTGCTGTCATGGGGAATCTGATTCCGGTCCGAGGGCGCCATT
ACTGGGAAGTGGAGGTGGCTGAGCATTTGGACTACACTGTGGGTGTGGCCTGTGAAGATGTCCCTAAGCA
GGAAGACCTGGGAGCAAACTCCTTGTCCTGGTGCATGAGGCATACATTTGTTTCCAAAAGGCACCGGTAT
GAGTTTCTGCACAACAAGATGACTCCAGACATCAGGATCACAGTTGCTCCGAGGAAGATTGGCGTGCTGC
TGGACTATGAGAACGCCAAGCTGTCCTTTTTCAATGTGGACATTGCTCAGCACCTGTACACATTTAGCTG
TCAGCTCCACCAGTTTGTGCATCCCTGCTTTTCCCTGGAAAAGTCTGGATGCCTAAAGATCTGCAATGGC
ATTTCAATGCCAAAGCATGTCACTTTCTTTTAGGCCCTTCTGGTATTTCCACTCTTTCTTCCTTCCTGTC
CCTCAGCCACCCTCCCCTCCCTGGCTGAACTTGGTATTCAAGGTTCCTACTGCTAGCCGAAGCTAAAGCT
CACGGTAGTGTCAGTAGATGGTGTACTGGAGCCCTTTTCACCTGTGTGCTGTGC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 67732. Forward Primer - name:067732_F_cDNA_9830160G03Rik, sequence:ATACAGCTGCAGCCTAGACACA; Reverse Primer - name:067732_N_SP6_cDNA_9830160G03Rik, sequence:GCACAGCACACAGGTGAAAAG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:12545 same embryo
 EMAGE:12546 same embryo
 EMAGE:12547 same embryo
 EMAGE:12549 same embryo
 EMAGE:12544 same embryo
 EurExpress:euxassay_011369 same experiment
 MGI:4824937 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS