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EMAGE:12563

Cnot8 CCR4-NOT transcription complex, subunit 8 ( MGI:1916375)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:12563 EMAGE:12563 EMAGE:12563 EMAGE:12563 EMAGE:12563
"Pseudo-wholemount" of euxassay_011364. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_011364_01 euxassay_011364_02 euxassay_011364_03 euxassay_011364_04
EMAGE:12563 EMAGE:12563 EMAGE:12563 EMAGE:12563 EMAGE:12563
euxassay_011364_05 euxassay_011364_06 euxassay_011364_07 euxassay_011364_08 euxassay_011364_09
EMAGE:12563 EMAGE:12563 EMAGE:12563 EMAGE:12563 EMAGE:12563
euxassay_011364_10 euxassay_011364_11 euxassay_011364_12 euxassay_011364_13 euxassay_011364_14
EMAGE:12563 EMAGE:12563 EMAGE:12563 EMAGE:12563 EMAGE:12563
euxassay_011364_15 euxassay_011364_16 euxassay_011364_17 euxassay_011364_18 euxassay_011364_19
EMAGE:12563 EMAGE:12563 EMAGE:12563 EMAGE:12563
euxassay_011364_20 euxassay_011364_21 euxassay_011364_22 euxassay_011364_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:12563Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
12563_wholemount_strong.wlz
12563_wholemount_moderate.wlz
12563_wholemount_weak.wlz
12563_wholemount_possible.wlz
12563_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:12563_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
lower jaw incisor
moderate moderate
regionalmoderate expression: see section 10 11 15 16
upper jaw incisor
moderate moderate
regionalmoderate expression: see section 11 15 16
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T30173
Entity Detected:Cnot8, CCR4-NOT transcription complex, subunit 8 ( MGI:1916375)
Sequence:sense strand is shown

>T30173
CGACTGCCGGAAGAAGAACACGAGTTCTTTCACATCCTGAATCTTTTCTTCCCGTCCATTTACGATGTGA
AATACCTGATGAAGAGCTGCAAAAACCTGAAGGGAGGCCTTCAGGAGGTGGCTGATCAGTTGGATCTGCA
GAGGATCGGAAGGCAGCATCAAGCAGGCTCAGACTCTCTGCTGACGGGGATGGCTTTCTTCAGGATGAAA
GAGCTATTCTTTGAGGACAGTATTGACGATGCCAAGTATTGTGGGCGCCTCTATGGCCTGGGCACAGGGG
TGGCCCAGAAGCAGAATGAGGATGTGGACTGTGCCCAGGAGAAGATGAGCATCTTGGCCATGATCAACAA
CATGCAGCAGTGACGGTCCGTGCACCATGCGGTCCGTGCACCATGCTGCCTGTGACTTGCATCTGCTCCA
AGCAGAGACTTTGGCTTTTTGTTTAAAGATGCCTTTATTTTAGATCCAGGAGGGGTTTGCTCTGAATTTG
TAAACAAGTCTTCCTCGTCCTCATGCCCCCACCCCTCTGCTGCCTCCTGCCACCAGCATCCATGGCCCCA
TGGACACTTTTTTAAATCTCCAGGATGTCTGAAACAAACCAGTAAAATGTCTATGACTCTTACATGTTGT
CATTCTTCTTCTTTTGGCTTTGCTGCTCTGATGAAGTTGCTCAGATGAGCTGTGGCTGCCAGAGAACAAG
GTGTATGTTCTTGGGAAGCAGGTCTATGCCAGTGGGCTGTTTTGCCTTGTTTGTGCTTCTTCAGGACAGA
GCAGTCCTGATGATACAGTAATTGATGGCAGACAGTTCTGTCTCCTTCCCTGTTGTTAGGCTGCCCCCCT
GAGCCTCACCTTGTTCACCTTGGTTTTCCGATGATGAGCTTCCCTAGACAGATCATGCTCCACGTCCACG
GTGTTCTCAGC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:3496188), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 14137. Forward Primer - name:014137_F_IRAV04-07_D17_Cnot8, sequence:CGACTGCCGGAAGAAGAA; Reverse Primer - name:014137_R_SP6_IRAV04-07_D17_Cnot8, sequence:TGCTGAGAACACCGTGGA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:12567 same embryo
 EMAGE:12564 same embryo
 EMAGE:12562 same embryo
 EMAGE:12565 same embryo
 EMAGE:12566 same embryo
 EurExpress:euxassay_011364 same experiment
 MGI:4823958 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS