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EMAGE:12622

Kctd8 potassium channel tetramerisation domain containing 8 ( MGI:2443804)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:12622 EMAGE:12622 EMAGE:12622 EMAGE:12622 EMAGE:12622
"Pseudo-wholemount" of euxassay_011387. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_011387_01 euxassay_011387_02 euxassay_011387_03 euxassay_011387_04
EMAGE:12622 EMAGE:12622 EMAGE:12622 EMAGE:12622 EMAGE:12622
euxassay_011387_05 euxassay_011387_06 euxassay_011387_07 euxassay_011387_08 euxassay_011387_09
EMAGE:12622 EMAGE:12622 EMAGE:12622 EMAGE:12622 EMAGE:12622
euxassay_011387_10 euxassay_011387_11 euxassay_011387_12 euxassay_011387_13 euxassay_011387_14
EMAGE:12622 EMAGE:12622 EMAGE:12622 EMAGE:12622 EMAGE:12622
euxassay_011387_15 euxassay_011387_16 euxassay_011387_17 euxassay_011387_18 euxassay_011387_19
EMAGE:12622 EMAGE:12622 EMAGE:12622
euxassay_011387_20 euxassay_011387_21 euxassay_011387_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:12622Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
12622_wholemount_strong.wlz
12622_wholemount_moderate.wlz
12622_wholemount_weak.wlz
12622_wholemount_possible.wlz
12622_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:12622_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
diencephalon lateral wall mantle layer
weak weak
regionalweak expression: see section 10 11 12 13 14 15 16
telencephalon mantle layer
weak weak
regionalweak expression: see section 02 03 04 05 06 07 08 16 18 19 20 21 22
medulla oblongata alar plate mantle layer
weak weak
regionalweak expression: see section 10 11 12 16
medulla oblongata basal plate mantle layer
moderate moderate
regionalmoderate expression: see section 13 weak expression: see section 08 09 10 11 12 14 15 16
rest of cerebellum mantle layer
weak weak
regionalweak expression: see section 07 08 09 10 11 19
pons mantle layer
weak weak
regionalweak expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20
midbrain mantle layer
weak weak
regionalweak expression: see section 18
tegmentum
weak weak
regionalweak expression: see section 09 10 11 12 13 14 15 16
facial vii ganglion
moderate moderate
regionalmoderate expression: see section 20 21 22 weak expression: see section 04 05
glossopharyngeal ix ganglion
weak weak
regionalweak expression: see section 07 18
trigeminal v ganglion
moderate moderate
regionalmoderate expression: see section 09 17 18 20 21 22 weak expression: see section 03 04 05 06 07 08 19
vagus x ganglion
weak weak
regionalweak expression: see section 09
vestibulocochlear viii ganglion
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 16 17 18 20 21 weak expression: see section 05 19
spinal cord mantle layer
weak weak
regionalweak expression: see section 11 12 13 14 15 16 17
dorsal root ganglion
moderate moderate
regionalmoderate expression: see section 09 10 11 12 16 17 18
neural retina
weak weak
regionalweak expression: see section 01 02 03
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T37766
Entity Detected:Kctd8, potassium channel tetramerisation domain containing 8 ( MGI:2443804)
Sequence:sense strand is shown

>T37766
CAGCCTAACACCTTAACCTTGGATAGACCCTCCAGGAAAGCACCTGTTCAGTGGATGCCACCACCAGACA
AGCGCAGAAACAGTGAACTCTTTCAGTCACTCATCAGCAAGTCCCGAGAAACAAATCTCTCCAAAAAGAA
GGTCTGTGAGAAGCTAAGTGTAGAAGAAGAAATGAAAAAGTGTATTCAGGATTTTAAAAAAATCCATATT
CCAGATTGTTTTCCAGAGCGCAAACGCCAGTGGCAATCTGAACTCCTCCAAAAATATGGGTTGTAATAAT
GATGATGCTCTGGGAGTGTCATGATGACATTCAGTGTTTACTGCAACGTCACCGCCTAACTGATGGCCGG
TGTGAATTCTTGCTGCTCTTTCCTTTTGTGAACAGTGGATGTGGGACAGTATTTTTTTCATTACTTTTAT
TGTTGTTTTTAGAAACATAATTTTAAAAATGAAACCAATAAATGTGTGTCAAAATGGTGTATCTATCTGA
TTCAAGCCATTTTGTAATAATGAACGTATAATGTGCATGATCAAGAAACTTAGAATCTTGATTTTTGAAC
AGTGGTTGACCGGACATGTTTGACATTCTGT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 71907. Forward Primer - name:071907_F_cDNA_Kctd8, sequence:CAGCCTAACACCTTAACCTTGG; Reverse Primer - name:071907_N_SP6_cDNA_Kctd8, sequence:ACAGAATGTCAAACATGTCCGGT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:12624 same embryo
 EMAGE:12623 same embryo
 EMAGE:12621 same embryo
 EMAGE:12620 same embryo
 EMAGE:12619 same embryo
 EurExpress:euxassay_011387 same experiment
 MGI:4825740 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS