EMAGE mouse gene expression spatial database

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EMAGE:12625

Trim45 tripartite motif-containing 45 ( MGI:1918187)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011322. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011322_01	euxassay_011322_02	euxassay_011322_03	euxassay_011322_04

euxassay_011322_05	euxassay_011322_06	euxassay_011322_07	euxassay_011322_08	euxassay_011322_09

euxassay_011322_10	euxassay_011322_11	euxassay_011322_12	euxassay_011322_13	euxassay_011322_14

euxassay_011322_15	euxassay_011322_16	euxassay_011322_17	euxassay_011322_18	euxassay_011322_19

euxassay_011322_20	euxassay_011322_21	euxassay_011322_22	euxassay_011322_23	euxassay_011322_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12625_wholemount_strong.wlz

12625_wholemount_moderate.wlz

12625_wholemount_weak.wlz

12625_wholemount_possible.wlz

12625_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12625_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

nasal cavity olfactory epithelium

strong

regional

strong expression: see section 08 09 10 11 12 13 15 16 17 18 moderate expression: see section 19 weak expression: see section 20

vomeronasal organ

strong

regional

strong expression: see section 12 15 16

axial skeleton

strong

regional

strong expression: see section 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9248
Entity Detected:	Trim45, tripartite motif-containing 45 ( MGI:1918187)
Sequence:	sense strand is shown >T9248 AGAATTAGCTGGGGACTAGCTCTAGAAGCGCTTGCGAGGAGGACGATGTGGGTGATCCAGGAGGGTGTGG CTGTGACTGGTGTGGGATGCAGTGGAAAGGAAGGGCCAAACCACAAATCCGGAGATCTGTGGATGGTTCC TCAGAGGCGTTCTCTTTCTGGTTGTCCTTTTACTTGGCAACACGAGAGTCCTCATGGTCAGTGTTCAAGA TCTTGGAACTCTGTCTTTGAGTAAATCTAACCTGCATTAGAGGACTGCGCAGGGCCTTTTGTGATACTGG GTACATACGTGGGGAAGATGTCAGAAATCAGGAAGCCGCTTCTGGGTTTCGTGCACAAACTCCAAGATGC GAACGCGTCTGGAAGCTCAGGCAAGACTCATTGTCCTACATGTCTGAGGCTTTTCAAAGTCCCCAGGCTC TTGCCTTGCTTGCACACAGTGTGCACCACGTGTCTGGAAAAGCTGGATCCATTCTCAGTAGTGGACATCC GTGGGGGTGACTCAGATACAAGCTCTGAGGGGTCAGTATTCCAGGACCCCGAGTTATGTAGCCTGCAGCC ACAGATCGGCATCCTCTGTCCGGTATGTGATGCTCAAGTGGACCTGCCCTTGGGTGGAGTGAAGGCTTTA ACGGTAGACCACCTGGCCATGAATGATGTGCTGCTGGAGAACCTGCGTGGGGAAGGCCAGGGCCTGGTGT GTGACCTGTGCAGCGACAGAGAAGTAGAGAAGAGGTGTCAGACCTGCAAAGCCAATCTCTGCCACTTCTG CTGCCAGGCTCATAGGCGACAGAAGAAGACGACATATCACACCATGGTGGACCTAAAAGACCTGAAAGGC TACAGCCAGGTTGGAAAGCCCATCCTATGTCCTTCTCACCCTGCAGAGGAGCTGAGGCTGTTCTGTGAAC TCTGTGACCGGCCGGTGTGTCGGGACTGTGTAGTTGGGGAGCACCGAGAGCACCCCTATGACTTCACCAG CAATGTCATCCACAAGCATGGAGATTCTGTGCGGGAGCTGCTTCGAGACACCCAGCCCCATGTGGGAGCC CTGGAAGATGCCCTGGCACAGATTAAAAGTGTGAACAACGCCCTCCAAGAGCGAGTGGAGGCAGTGGCAG CTGACGTCCGGACATTCTCTGAGGGCTACATCAAAGCCATCGAGGAACACCGGGACAAGCTGCTGCAGCA GCTGGATGACATACGGATCCAGAGGGAAACCGCTTTACAGCTGCAGAAAGCGCAGCTGGAACAGCTGCTG GCAGATATGCGGACTGGAGTGGAGTTCACCGAGCATCTGCTGACCAGCGGCTCGGACTTAGAGATTCTCA TCACCAAGGGAGTGGTTGTAGAGCGGCTCAGGAAGCTGAATAAAGTTGAATACAGTGCCCGCCCAGGAGT GAACCATAAGATCTGCTTCTCCCCCCAGGAGAAGGCAGGCCAGTGCCAAGGCTATGAAGTGTATGGGGCC ATTAACACGCAGGAAGTTGATCCAGCTCAGTGTGTCCTTCAGGGAGAAGATCTCCACAGAGCTCGAGAGA AACAGACAGCCTCTTTTACCTTGTTCTGTAAGGATGCCTCGGGACAGAGCATGGGCAGGGGAGGAGATAA TGTCCATGTGGAAGTTGTCCCCAAAGATAAGAAGGACAGTCCGATCAGAACAGTGGTCCAGGACAACAAG GATGGGTCGTACCGCGTTTCCTACACCCCCAAGGAGCCTGGCATCTACACCGTGTGGGTCTGCATCAGAG AGCAGCACGTGCAGGGCTCACCATTCAACGTGACCGTGAGGAGGAAGCATCGGCCACATCCGGGTGTGTT TCACTGCTGCACCTTCTGCTCCAGTGGAGGACAGAAAGCTGCCCGCTGTGCCTGCGGAGGCACCATGCCG GGTGGATACCTTGGCTGTGGCCACGGACACAAAGGCCACCCGGGCCGTCCACACTGGTCTTGCTGTGGGA AGTTCATTGAGAAGTCCGAGTGCTCGTTCACGAGTGGGCAGGGCGCCCCGAGGAGTCTGCTTAGGACCGT GGCGCTCTGATGCTTCCTGGGGACAAGTCGGGCCAGGTAACCCTGAAGCTAGCATAACTTCAGCTATGCA GAGAACCCAGAGCTTGAGTAGTTCCAAAGAGACTGACAGAATTAAAGACGAAGTGCTTTCTCTAACATCA GACTTCAAGTGCTTTCTGTGTGGGCATCTCTCGTGTACCATGGCGGAGTGCAGGCCTCCCCTACACCGCT CCTCAAGGCAGGCTGCTGATCTACTCCACGGAAAGCTCTCCTGTTTTATACGGGCATTGAGGTGTCTGTG CCATGGGACCATGAGCTCCTGGAGCAGGGAGGACTTTATGCCTTCCTTTCCACTGTGTTGCAGCCTCTCC GGTTTACCCCGAGCTAGAGTTTCAGGGCTTCTCCTGGAGGATGAAACCCCTCTGGCCCATCATTTGTTTG TTGTATACTTTCAGTGGTTTCCTAGTACACAGTTTATGATGATTATCACGGTTTTGCCGTCTAGATGAGC GCCCGAGTGATGCTCATGTGTAGGCACACAAGTAGACAGTAAGGAGGTACGGTGTGGCAAGAGCGCAGCC ATCTGCTGCAGTTCTTTACATCAGATGACGGCGGGTCCTCCATCCACACAGCTCTGACTTAATGGAGGGC TTTCGCTGCCCTCTCTACCCTGCAACAGCTGAAAGCACTGACTCTGCAGGGCACCCCCTAGCGGCTACAG GATTCTGGGAGGAAGACTTAGTACTCCCTCAGCCCAGCCAAGGTCTTCCCAGTAGAGATTTTGTGTTTTC TGGGATTCTTGGTTTCTTACACAGCAGTAAACTCACAACATATGAAACTGAATTACTGAAGTTTGCATTA TAGGATCTCATAGGGAGTGGTGACTCATTGGAGGGGCAATTGGGTAAAGGAAAGGAAGCGAGGCACTTAG ATGACCACTGTCAGGTTTCACTTCCTTCTCTTAGCCTTTAAGATAAGATACATTAAGATAATTAAGACAT TAAGATACTTTCTTCATTTTCTTCGAACATTCCCCTTGAGCCCCTTTCTGGAAGCAAAATCTGATGTTCC CAGAAGACAACACTGCCCCTCTCCCCCTCAGCTGGTTGTTGTTTGCTCCTCACTCTGTCTGTTCCCACCC ACTCCCAGCCACACCCTGAGCCAGGTCAGGTGTCTACTGAGCTTGCCATGGCTGCTTTGAGATCACTGGT TCTCAGCCAGGCAACCCCACCTCCCGGGGGCGTGTCTTACAGAATCTTGGGGATATGGGTTCTAGCAGCA TTGAGTAGGGCCACAGGTCAGTTTATTCAACTGCAGGATAGCTTTCTGCAGCAAATGGAGCATGCTTTGC CTCCCTGCAGTGCCATTTTCATGTCTGATGAGGCGTACATCCTGCATTCTGCTCAATGGTTCCCTCCCAG GCTCCTAAGCTCGACCTTGCACTGTGTTGTTGTCAGTATGCTCAGTAGAGTCAAAGAGCAGTCCTTCCAT TTCTCGCCCCTGTTTCCTCCCGTGACCTCCTTCTGACTTAAATACCACTTAGACTCGGTGACTCCCAAGT CCTCTTCAGGTCACTCTCTAGATGATTATGAGGTCCAGTGACTTACGTCATCACATCTCCTCTTATGTGT ACAGTAAGCCCCTCATTCAAATTTAAATGGCTCCCAGTGTCCAGTTCTCACTTGCTCCTGCCTGTCATCA CCAAACAGCTTCTCCTGGAGTCCTCTCCACACTTTAAAAGAGAAGCGTCTGTCCTTAGGTGGGGCAGCCT GGAAACTCCTTGTGTGTCACACCATATCCAAACCACTGGTAACTCCTGTCAACCTCACTTTCACTGACTC CTGCTCTCTGCGTCCATTGCTACCATCCTGGTCTGACAAGCCGAGACTCCCACCAGAAAGCTTTGCCTAT CTTCTCGCCAGCCTGCTTCCACATCAGGTCATTCTGCTCGGTGCCTCCAGTGACCGCTCGCTCATCTCAG AACAGAGGCAGCTCACCTGCTTCACATTACCATGTGCCAGCGTGGTCTGATCCATGGCCTTCTCTTCCTA CCCAGCTCCACCTTCTGTCCAGGCACAGTGGCCCTGCCGGCCGCTGCTCAGGCTCAGCAGCCTCTTCTTG GAGCCTTTATAGTAATGGGCCTTCTATCTGACAGTCCTTCTCAAAGATGCATCATACCCTTTGACTTCAA GTTTCTGCTCTAGTGTCAACTATTGTCCTATTAGTGAGGTTTCTAACCATCCTGAGTAAAACAGAAGAAT CATACCCAGGAGGAGGCATCCCCTGCCCGGCTTCATCACCAGCTGACGGGTGATGTATTGTCTTACATCT GCTGTACTGGACTCTGAGCTCCGCGAGGGCAGGGAGCTGGCATTCAGTAACTGTATTATCCCCATGCAGG TCAGACTTTGGCTCACAGTAGAGACAAATAGTATGTTGAATGAATAAAAATTCAAGTTGGTAAAAAAAAA AAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6835531 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6835531 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12626 same embryo
	EMAGE:12629 same embryo
	EMAGE:12627 same embryo
	EMAGE:12628 same embryo
	EMAGE:12630 same embryo
	EurExpress:euxassay_011322 same experiment
	MGI:4828916 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source