EMAGE mouse gene expression spatial database

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EMAGE:12703

Nek5 NIMA (never in mitosis gene a)-related expressed kinase 5 ( MGI:2142824)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


euxassay_011536_01	euxassay_011536_02	euxassay_011536_03	euxassay_011536_04	euxassay_011536_05

euxassay_011536_06	euxassay_011536_07	euxassay_011536_08	euxassay_011536_09	euxassay_011536_10

euxassay_011536_11	euxassay_011536_12	euxassay_011536_13	euxassay_011536_14	euxassay_011536_15

euxassay_011536_16	euxassay_011536_17	euxassay_011536_18	euxassay_011536_19	euxassay_011536_20

euxassay_011536_21	euxassay_011536_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12703_wholemount_strong.wlz

12703_wholemount_moderate.wlz

12703_wholemount_weak.wlz

12703_wholemount_possible.wlz

12703_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12703_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata part of 4th ventricle choroid plexus

weak

regional

weak expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

diencephalon roof plate

weak

regional

weak expression: see section 11 12 13

choroid invagination

weak

regional

weak expression: see section 05 06 07 08 09 10 14 15 16 17 18 19

metencephalon part of 4th ventricle choroid plexus

weak

regional

weak expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30374
Entity Detected:	Nek5, NIMA (never in mitosis gene a)-related expressed kinase 5 ( MGI:2142824)
Sequence:	sense strand is shown >T30374 GCAGAGGATCCAGAGGCAGCGGGGAGTGATGTTCAGCGAAGACCAGATCCTGTGTTGGTTTGTACAGATT TCTCTAGGACTGAAGCATATTCATGACAGGAAGATTTTACACAGGGACATAAAATCTCAGAATATTTTTC TTAGCAAGAATGGAATGGTTGCCAAGCTCGGGGACTTTGGAACAGCAAGAACACTGAATGACTCCATGGA ACTTGCTCAAACATGTGCTGGGACACCTTACTACCTGTCCCCAGAGATCTGTCAGAACAGGCCATACAAC AATAAAACGGACATCTGGTCTCTTGGCTGTGTCTTATATGAGCTCTGCACACTCAAGCATCCTTTTGAGA GCAACAACTTCCACCATCTGGTTCTGAAGATTTGTCAAGGACGTGTTGCTCCCATATCACCCCACTTCTC TCGTGACCTACAGTCCTTGATACCTCAGCTCTTCAGAGTGTCTCCTCAGGACCGGCCATCCGTTACGTCC CTTTTGAAAAGACCCTTTTTAGAAACTCTCATTGCCCGATCCTTGTATCCTGAGGTCTGTTCAAGAAGAA TCCAGTCCCACGCTCACATGGAGAACATGGCCATTGGCCCCACAGCTTGTTGGAGAGTTAGTCCATGGTC TGCTGCCTACCTTCAGAGGAAATTTGAAGCTCAGCAATATAAGTTAAAAGTGGAAAGACAATTGGGTCTT CGTCCATCTTCTGTGGAACCACATCCAAACGAGGGAGAAAAGCTACAAAGTCATTGGGAGGAGACTAAAT TCCAGGAGCTTCAATACAGGAAGAACAAAATGAAGGACCAGGAATATTGGAAACAGCTGGAAGAAATCCG CCA
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:6311513), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 58213. Forward Primer - name:058213_F_IRAV101_h03_BB049667, sequence:GCAGAGGATCCAGAGGCA; Reverse Primer - name:058213_R_SP6_IRAV101_h03_BB049667, sequence:TTGGCGGATTTCTTCCAG. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12705 same embryo
	EMAGE:12704 same embryo
	EMAGE:12708 same embryo
	EMAGE:12707 same embryo
	EMAGE:12706 same embryo
	EurExpress:euxassay_011536 same experiment
	MGI:4826662 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source