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EMAGE:12723

Aldh1b1 aldehyde dehydrogenase 1 family, member B1 ( MGI:1919785)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:12723 EMAGE:12723 EMAGE:12723 EMAGE:12723 EMAGE:12723
"Pseudo-wholemount" of euxassay_011652. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_011652_01 euxassay_011652_02 euxassay_011652_03 euxassay_011652_04
EMAGE:12723 EMAGE:12723 EMAGE:12723 EMAGE:12723 EMAGE:12723
euxassay_011652_05 euxassay_011652_06 euxassay_011652_07 euxassay_011652_08 euxassay_011652_09
EMAGE:12723 EMAGE:12723 EMAGE:12723 EMAGE:12723 EMAGE:12723
euxassay_011652_10 euxassay_011652_11 euxassay_011652_12 euxassay_011652_13 euxassay_011652_14
EMAGE:12723 EMAGE:12723 EMAGE:12723 EMAGE:12723 EMAGE:12723
euxassay_011652_15 euxassay_011652_16 euxassay_011652_17 euxassay_011652_18 euxassay_011652_19
EMAGE:12723 EMAGE:12723 EMAGE:12723
euxassay_011652_20 euxassay_011652_21 euxassay_011652_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:12723Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
12723_wholemount_strong.wlz
12723_wholemount_moderate.wlz
12723_wholemount_weak.wlz
12723_wholemount_possible.wlz
12723_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:12723_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
pancreas
strong strong
regionalstrong expression: see section 08 09 10 11 12 14 15
midgut
moderate moderate
regionalmoderate expression: see section 06 07 08 09 10 11 12 13 14
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T32007
Entity Detected:Aldh1b1, aldehyde dehydrogenase 1 family, member B1 ( MGI:1919785)
Sequence:sense strand is shown

>T32007
AGGCAGCTGGCGAGTCTAACCTCAAGAGAGTCACCCTGGAGCTGGGTGGGAAGAGCCCCAGCATTGTGCT
GGCAGACGCTGACATGGAGCATGCCGTAGATCAGTGTCACGAAGCCCTTTTCTTCAACATGGGCCAGTGC
TGCTGTGCAGGCTCCCGGACATTCGTGGAAGAGTCCATCTACCGTGAGTTTCTCGAGAGAACTGTGGAGA
AGGCCAAGCAGAGGAAAGTGGGGAACCCCTTTGAGTTGGACACCCAGCAGGGACCTCAGGTGGACAAGGA
GCAGTTTGAACGAATCCTGGGCTACATCCGGCTGGGACAGAAGGAAGGGGCAAAGCTTCTCTGTGGCGGG
GAGCGTTTGGGGGAGCGCGGCTTCTTCATCAAACCCACAGTCTTCGGGGACGTTCAGGATGGCATGAGGA
TCGCCAAGGAGGAGATCTTTGGGCCCGTGCAGCCTCTGTTCAAGTTCAAGAAGATCGAGGAAGTAATCCA
GAGAGCCAACAACACCAGGTATGGCCTGGCTGCGGCTGTGTTCACCCGAGACCTGGACAAGGCCATCTAC
TTCACGCAGGCCCTGCAAGCTGGGACGGTGTGGGTGAACACCTATAACATTGTCACCTGCCACACGCCAT
TCGGAGGCTTTAAGGAATCTGGCAATGGCAGGGAGCTGGGGGAGGACGGGCTCAGAGCCTACACGGAGGT
GAAGACTGTCACCATCAAGGTTCCCGAGAAGAATTCCTGAGATTGGCCATCTTGGAGGCCCAGCCCTGTC
CTGCAGCTCCGTGACATCTAGCCAGTGGAGGGCAAAATCTGATTTCAGCCTGAGTTCCCAGTGAAGTGTT
ACAAGAAGTGTCAACCAATAAAGTAGTCCAGCCAGAGCTTTTCTACTTAAAGCAGAGACGGTGGCTGGGC
CCAGAGTGCAGCCCATTTCATC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4207479), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 22876. Forward Primer - name:022876_F_IRAV32-35_J03_Aldh1b1, sequence:AGGCAGCTGGCGAGTCTA; Reverse Primer - name:022876_R_SP6_IRAV32-35_J03_Aldh1b1, sequence:GGATGAAATGGGCTGCAC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:12724 same embryo
 EMAGE:12726 same embryo
 EMAGE:12722 same embryo
 EMAGE:12725 same embryo
 EurExpress:euxassay_011652 same experiment
 MGI:4823081 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS