EMAGE mouse gene expression spatial database

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EMAGE:12743

C8g complement component 8, gamma polypeptide ( MGI:88237)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011571. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011571_01	euxassay_011571_02	euxassay_011571_03	euxassay_011571_04

euxassay_011571_05	euxassay_011571_06	euxassay_011571_07	euxassay_011571_08	euxassay_011571_09

euxassay_011571_10	euxassay_011571_11	euxassay_011571_12	euxassay_011571_13	euxassay_011571_14

euxassay_011571_15	euxassay_011571_16	euxassay_011571_17	euxassay_011571_18	euxassay_011571_19

euxassay_011571_20	euxassay_011571_21	euxassay_011571_22	euxassay_011571_23	euxassay_011571_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12743_wholemount_strong.wlz

12743_wholemount_moderate.wlz

12743_wholemount_weak.wlz

12743_wholemount_possible.wlz

12743_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12743_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

liver

moderate

regional

moderate expression: see section 01 02 03 05 06 07 09 10 11 13 14 15 16 17 18 19 22 23 24 weak expression: see section 04 08 12 20 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T31016
Entity Detected:	C8g, complement component 8, gamma polypeptide ( MGI:88237)
Sequence:	sense strand is shown >T31016 GACACAATGCCCAGCTCCTATCAAGGCTAGGTTCTAGGATGGACCCTGGCTTGGGTTAGGGGTTCAGTAA TCGGAAGACAGATGTGGACTTCCTGGACTGTGCTTCCTGTGGCACTGGACAGTGGACTCTGACCTAGGAC AGTGCACCCCTCCACCTCGCTGTCCTGTCACCTACTGTCCGTAGCAGGTTGGCCATGCTGTCTCCAGGGG CTGTGTTGTTCTTCACCCTGCTCTTGACAGCCAGTTCGCTGGGCCAAAGGACTCGAAAGCCTATTGGGTC TACCTCCCCCATCAGCACTATCCAGGCCCAGGTCAATTTCAGTGCTCAGAAGTTTGCAGGGACATGGCTC CTGGTGGCTGTGGGCTCTTCGTGCCGCTTTCTTCAGGAGCAGGGACACCGAGCTGAGGCCACCACATTGC ACGCAGCTCCCCAGGGTGCAGCTATGGCTGTCAGCACCTTCCGAAAGCTAGATGGGATATGTTGGCAGGT ACGCCAACTCTTTGAGAACACAGGGGTCCCTGGCCGCTTCTTGTTCCAAGTCTCACGGGCCCGTGGACCA GTGCATATGGTGGTTGCTGAGACTGACTATCAGAGCTTTGCCATCCTGTACCTGGAACAGGGAAGGAAGC TGTCTGTGAAACTATACGTCCGCTCACTGCCAGTGAATGACTCTGTCCTGGATGTGTTTGAGCGGCGAGT CAGGGAAGCCAACCTGACAGAAGATCAGATTCTTTTCTTTCCCAAGTATGGTTTCTGCGAGACTGCGGAC CAATTACACATCCTGAACGAGGTGCCGCGATAAGGCCATCTAGTGGAGAAGCTGTGGACAGTGGG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4023907), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 22511. Forward Primer - name:022511_F_IRAV32-35_O18_1700013L23Rik, sequence:GACACAATGCCCAGCTCC; Reverse Primer - name:022511_R_SP6_IRAV32-35_O18_1700013L23Rik, sequence:GCCCACTGTCCACAGCTT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12744 same embryo
	EMAGE:12740 same embryo
	EMAGE:12742 same embryo
	EMAGE:12739 same embryo
	EMAGE:12741 same embryo
	EurExpress:euxassay_011571 same experiment
	MGI:4823565 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source