EMAGE mouse gene expression spatial database

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EMAGE:12789

Mob3b MOB kinase activator 3B ( MGI:2664539)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011555. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011555_01	euxassay_011555_02	euxassay_011555_03	euxassay_011555_04

euxassay_011555_05	euxassay_011555_06	euxassay_011555_07	euxassay_011555_08	euxassay_011555_09

euxassay_011555_10	euxassay_011555_11	euxassay_011555_12	euxassay_011555_13	euxassay_011555_14

euxassay_011555_15	euxassay_011555_16	euxassay_011555_17	euxassay_011555_18	euxassay_011555_19

euxassay_011555_20	euxassay_011555_21	euxassay_011555_22	euxassay_011555_23	euxassay_011555_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12789_wholemount_strong.wlz

12789_wholemount_moderate.wlz

12789_wholemount_weak.wlz

12789_wholemount_possible.wlz

12789_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12789_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

lower jaw incisor

strong

regional

strong expression: see section 15 moderate expression: see section 16 19

lower jaw molar

strong

regional

strong expression: see section 10

upper jaw incisor

strong

regional

strong expression: see section 15 moderate expression: see section 18 19

upper jaw molar

strong

regional

strong expression: see section 10 21

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30996
Entity Detected:	Mob3b, MOB kinase activator 3B ( MGI:2664539)
Sequence:	sense strand is shown >T30996 GGCTGAAAGCGATCCTGACTTCCAGGAGCCGGAAAGCCCCACAAGGACCTCTGAAAGACATCTTTTACCC ACTTCTCGTGCTCTTAACCCTCTAAGTGCTGCTAGCCAGGACCTGTGGGCCATGTAGACCACAGCATGAA GGACCAGCCTTAACCCCTTGGGCCGAGGACAGTGCAAGAGAGGCTGCTGCACTTCCGCCCACGTCACACT TCTGCCTGTGGCCTGCCCGCTAGGCTTTACTGGAATTCAGGTTTTGAGACTGGGGGGTTTGTTGTGGTGG TTCTTTTCCACGTCCGTCTTCTTGACTAACTTCTCTGTCACTTATTTTTCGAAGTTCTAAGCAAACGTGG AGCTACCATATAGGCAGTACGTTCTCCTATCCCGTCTCTCCTCCACTCCCCGCAGGTCTCTGGGCCTATG GCTTAACCTTACATGGGGCTTGGAGCTGGGGAAAGCCTGCAGTTTCTCTGGAAGCATCCAAGCCGTTTCC CTCATGGGCAGTTTCACCAGGGACCACTTTCTTGATGGGCTACATGACCTGTACCCCAGACAGGCCATTC CCAGGCTACCCTTTGGGACGAGTCTAGATTTATTCTACAGGAAATAAAGCTGGTGTGTGATCTGTTTACC CTAGTCAGAATGTATTCTTTGTAGCCGCAGTCATTTCTAGATGCTTCCATTAAAGATATGCTTTCCCATT TCCCAGAAGAGAGGGAATAAAGTTTTTAAATGACCAGTAAGATCCTTGAGCCAGATTAGTGCAGTGTTTG TTCTTTATTAAGAGGTAGTGAGTCCTGGCTGATCCTACATATTATATTATGGGGGCTTCAGG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4007373), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 22181. Forward Primer - name:022181_F_IRAV32-35_G04_Mobkl2b, sequence:GGCTGAAAGCGATCCTGA; Reverse Primer - name:022181_R_SP6_IRAV32-35_G04_Mobkl2b, sequence:CCCTGAAGCCCCCATAAT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12790 same embryo
	EMAGE:12791 same embryo
	EMAGE:12792 same embryo
	EurExpress:euxassay_011555 same experiment
	MGI:4826419 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source