EMAGE mouse gene expression spatial database

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EMAGE:12804

Slc34a1 solute carrier family 34 (sodium phosphate), member 1 ( MGI:1345284)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011507. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011507_01	euxassay_011507_02	euxassay_011507_03	euxassay_011507_04

euxassay_011507_05	euxassay_011507_06	euxassay_011507_07	euxassay_011507_08	euxassay_011507_09

euxassay_011507_10	euxassay_011507_11	euxassay_011507_12	euxassay_011507_13	euxassay_011507_14

euxassay_011507_15	euxassay_011507_16	euxassay_011507_17	euxassay_011507_18	euxassay_011507_19

euxassay_011507_20	euxassay_011507_21	euxassay_011507_22	euxassay_011507_23	euxassay_011507_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12804_wholemount_strong.wlz

12804_wholemount_moderate.wlz

12804_wholemount_weak.wlz

12804_wholemount_possible.wlz

12804_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12804_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

rib

strong

regional

strong expression: see section 03 04 moderate expression: see section 05 06 07 08 09 10 11 12 21 22 23 24

humerus

moderate

regional

moderate expression: see section 05

femur

strong

regional

strong expression: see section 07 moderate expression: see section 08 09 22 23

mandible

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 18 19 20 21 22 moderate expression: see section 04 23 24

maxilla

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 15 17 18 19 20 21 22 moderate expression: see section 04 23 24

metanephros

strong

spotted

strong expression: see section 09 10 11 12 20 21 22 23 24

axial skeleton

strong

regional

strong expression: see section 17 18

basioccipital bone

strong

regional

strong expression: see section 07 21

orbito-sphenoid

strong

regional

strong expression: see section 01 02 03 04 05 06 22 23 moderate expression: see section 24

clavicle

strong

regional

strong expression: see section 05 06 07 12 19 20 moderate expression: see section 08 09 10 11 21 22 23 24

scapula

moderate

regional

moderate expression: see section 05

pelvic girdle skeleton

moderate

regional

moderate expression: see section 11 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T30127
Entity Detected:	Slc34a1, solute carrier family 34 (sodium phosphate), member 1 ( MGI:1345284)
Sequence:	sense strand is shown >T30127 AGCCCCAGGGAGAAGCTATCCAGCTCATTTCAGATTGCCCTCTGCCACTTCTTCTTCAACATCTCGGGCA TCCTACTGTGGTACCCGCTGCCCTGCACACGCCTGCCCATACGCATGGCCAAGGCCCTGGGCAAACGCAC CGCCAAGTACCGCTGGTTTGCTGTCCTCTACCTGCTCGTGTGCTTCCTGCTCTTGCCCTCGCTGGTGTTT GGCATTTCCATGGCAGGCTGGCAGGCTATGGTCGGAGTGGGCACGCCCTTTGGGGCTCTACTGGCCTTTG TGGTGCTTGTTAATGTCCTGCAGAGCCGAAGTCCCGGGCACCTCCCCAAGTGGCTGCAGACATGGGACTT CCTACCTCGCTGGATGCACTCTTTGCAGCCCCTGGATGGCCTTATCACAAGAGCCACCTTGTGTTATGCC AGGCCCGAGCCCCGTTCACCCCAACTTCCCCCTAGAGTCTTCCTGGAGGAGCTTCCCCCAGCCACGCCCT CCCCCCGCCTGGCACTGCCTGCTCACCACAATGCCACCCGTCTCTAGGCTACAGGCCCCGACTACTGCCT GGAACGAGGAGTGGTATTTTTACAATGCCCACAGTAGAAAGACAGAGCATGTGTGTGTGTGTGCACACAT GTGTAGATGCGTGTCACTTGAGGTACATGCTTTTGTGCCACCCTTGGGTACCTGTGCATATTAAGGGCCA CAGTGGCATGGGCTTTTG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4223484), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 17660. Forward Primer - name:017660_F_IRAV28-31_E04_Slc34a1, sequence:AGCCCCAGGGAGAAGCTA; Reverse Primer - name:017660_R_SP6_IRAV28-31_E04_Slc34a1, sequence:ACAAAAGCCCATGCCACT. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12806 same embryo
	EMAGE:12805 same embryo
	EurExpress:euxassay_011507 same experiment
	MGI:4828195 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source