EMAGE mouse gene expression spatial database

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EMAGE:12845

Tprgl transformation related protein 63 regulated like ( MGI:1915058)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011614. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011614_01	euxassay_011614_02	euxassay_011614_03	euxassay_011614_04

euxassay_011614_05	euxassay_011614_06	euxassay_011614_07	euxassay_011614_08	euxassay_011614_09

euxassay_011614_10	euxassay_011614_11	euxassay_011614_12	euxassay_011614_13	euxassay_011614_14

euxassay_011614_15	euxassay_011614_16	euxassay_011614_17	euxassay_011614_18	euxassay_011614_19

euxassay_011614_20	euxassay_011614_21	euxassay_011614_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12845_wholemount_strong.wlz

12845_wholemount_moderate.wlz

12845_wholemount_weak.wlz

12845_wholemount_possible.wlz

12845_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12845_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 17 weak expression: see section 08 09 10 16

pons mantle layer

weak

regional

weak expression: see section 07 18

facial vii ganglion

moderate

regional

moderate expression: see section 04 05 06 07 21 22

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 07 18 19

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 09 18 19 20 21 22

vagus x ganglion

moderate

regional

moderate expression: see section 08 18

trigeminal v nerve

moderate

regional

moderate expression: see section 10 17

ventral grey horn

moderate

regional

moderate expression: see section 12 weak expression: see section 10 11 13 14 15 16 17

dorsal root ganglion

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 16 17 18

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 12 13 15 16 17 18 weak expression: see section 09 10 11 19 20

vomeronasal organ

moderate

regional

moderate expression: see section 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T31006
Entity Detected:	Tprgl, transformation related protein 63 regulated like ( MGI:1915058)
Sequence:	sense strand is shown >T31006 CCTTCAGTGCCAGCAGGTGGTCAGGGTGGCGCTCAGCGCGGTAGACACCATCTCCTGTGGAGAATTCCAG TTTCCTCCGAAGTCGCTCAACAAGAGAGAAGGCTTTGGGGTTCGCATTCAGTGGGACAAGCAGAGCCGCC CTTCCTTCATCAACAGATGGAACCCCTGGTCTACAAACATGCCCTATGCCACCTTCATAGAACACCCAAT GGCTGGCATGGACGAGAAGACAGCGTCTCTGTGCCATTTGGAAAGCTTCAAGGCTCTCCTGATCCAAGCT GTCAAGAAGGCCCAGAAGGAGAGCCCACTGCCAGGGCAGGCGAATACCGTGCTGGTGCTGGAGCGCCCCC TCCTCATCGAGACCTACGTGGGGCTCATGTCCTTCATCAACAATGAAGCCAAGCTGGGCTACTCCATGAC CAGGGGCAAGATAGGCTTCTAGACAGCTGCGTCCCGGAAACCAAGGTGGTGGGGACTCCCTGTGCCACCT CATGCCACCCACTGGCTGGAGGGAAGGAGGGCATGTATCCGATTAGAATAGTCAAGTGGATTATGTATAT TTAGTTGGATATTGGACATCTTGGCAACACAATCTTACAAAGGCAGATTCAGCTGCCCCCATACCACTGT GTTCCTTCTGTAACGCCACGTTCTCCCTCCAGCATGCCGAGCACAATGTCCACCCACTTGTGCTGTGTAG ACTGTTGCATACAGCTGAGTACAGTGAGCGGCTTCTGCACATATTTAACTCTCCCTATATATTAACTCTC AAGTGCTGTATTTTAAAATTCAGCCCGTTTTTTAAAAAAGCTAAATACAGTAGCAGCCAGCAGCCAGTCA CTAAGAAAGACAGTCTGCCTGGCAGCTCCTGGTGAACCTGCCTTTCTGTGAATGTGTCTGACTTAGAATC ACACTTTATAAATTAATCCAGGTTGTGTTAATACAGCAGTTTTCCTAGGCCCAAAAGGTTACTCTCATAA CATAAGCTAGCCCCTTCCG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4211201), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 22921. Forward Primer - name:022921_F_IRAV32-35_J13_1200015A19Rik, sequence:CCTTCAGTGCCAGCAGGT; Reverse Primer - name:022921_R_SP6_IRAV32-35_J13_1200015A19Rik, sequence:CCGGAAGGGGCTAGCTTA. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12848 same embryo
	EMAGE:12847 same embryo
	EMAGE:12844 same embryo
	EMAGE:12846 same embryo
	EurExpress:euxassay_011614 same experiment
	MGI:4828884 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source