EMAGE mouse gene expression spatial database

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EMAGE:12852

Arl6ip6 ADP-ribosylation factor-like 6 interacting protein 6 ( MGI:1929507)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_011620. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_011620_01	euxassay_011620_02	euxassay_011620_03	euxassay_011620_04

euxassay_011620_05	euxassay_011620_06	euxassay_011620_07	euxassay_011620_08	euxassay_011620_09

euxassay_011620_10	euxassay_011620_11	euxassay_011620_12	euxassay_011620_13	euxassay_011620_14

euxassay_011620_15	euxassay_011620_16	euxassay_011620_17	euxassay_011620_18	euxassay_011620_19

euxassay_011620_20	euxassay_011620_21	euxassay_011620_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

12852_wholemount_strong.wlz

12852_wholemount_moderate.wlz

12852_wholemount_weak.wlz

12852_wholemount_possible.wlz

12852_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:12852_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

submandibular gland primordium

weak

regional

weak expression: see section 09 10 11 18 19 20 21

olfactory cortex ventricular layer

moderate

homogeneous

moderate expression: see section 12 13 17 18 19

telencephalon ventricular layer

moderate

homogeneous

moderate expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 16 17 18 19 20 21 22 weak expression: see section 01 02

midbrain ventricular layer

moderate

regional

moderate expression: see section 13 14 15 weak expression: see section 09 10 11 12 16

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 10 11 12 13 14 16 17 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T31007
Entity Detected:	Arl6ip6, ADP-ribosylation factor-like 6 interacting protein 6 ( MGI:1929507)
Sequence:	sense strand is shown >T31007 TCGTCGGAGACCAAGAGGGCCCCGCTACTCCCCCGGGTCGGGGACGGGTCTCCGGTCCTGCCGGATAAGC GCAACGGCATCTTCCCGGCCACTGCGGCCAAGCGAACCCAGGCTCGGCGGTGGCCCATCCAAGCCCTTTC CATTCTCTGTTCGCTGCTGTTCGCCGTCCTCCTCGCTTTCCTTCTCGCCATCGCCTACATGATCGTTAAA GAATTGCATGCAGAAAACTTGAAAAATGAAGATGATATACATACTGGGCTGTTAGGCTTCTGGTCTCTGC TGATAATATCTCTAACTGCTGGATTGTCCTGTTGCAGCTTTTCTTGGACAGTGACTTACTTTGATTCTTT TGAACCAGGAATGTTTCCACCCACTCCTCTTTCACCTGCTAGGTTCAAGAAACTGACTGGACATTCTTTC CACATGGGCTACAGCATGGCAATTTTGAATGGTGTTGTCGCTGCTCTCACTGTGGCATGGTGCCTCATGT AAACCTACACTAGAGCAACGTTGTTGGTGAAAGTCATGATGGTTTTTGAAGTAGCAGAAACGAAATTGCT GACTCAGAAGACTTTGAAATGTGATCAGATACTTACTATCATCATTATGGAAGACCTTATAGAGGGCACT TCTTCAATGTATAAGATGTTTACTTCATCTTTTTACTTTTGTTTATGTAGTTTATCAGTTACAGAAAATA TTTGAATTGAAATCAAACTTGGAAACTTGAATACATGACATTGCCTACCTTTCTATGTATATTCCATTTT TTACTAACAGATACAGATATTTGTGGTTGAGGTAGAAATATTTATATACTATATTAGGAAAACAGTACTG AGGCCAAGTGTGTTAGCAAGACCTTCAAGCCC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from the plasmid IMAGE:4210432), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 22911. Forward Primer - name:022911_F_IRAV32-35_J15_Arl6ip6, sequence:TCGTCGGAGACCAAGAGG; Reverse Primer - name:022911_R_SP6_IRAV32-35_J15_Arl6ip6, sequence:TGGGCTTGAAGGTCTTGC. The reverse primer contains a 5' extension containing an SP6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using SP6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:12853 same embryo
	EMAGE:12851 same embryo
	EMAGE:12849 same embryo
	EMAGE:12850 same embryo
	EurExpress:euxassay_011620 same experiment
	MGI:4823243 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source